Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NGC85_RS15135 Genome accession   NZ_CP101115
Coordinates   3199263..3200975 (+) Length   570 a.a.
NCBI ID   WP_254732215.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3194263..3205975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS15100 (NGC85_15100) nusA 3195091..3196575 (-) 1485 WP_254732212.1 transcription termination factor NusA -
  NGC85_RS15105 (NGC85_15105) rimP 3196613..3197137 (-) 525 WP_254732213.1 ribosome maturation factor RimP -
  NGC85_RS15125 (NGC85_15125) secG 3197760..3198089 (-) 330 WP_005061069.1 preprotein translocase subunit SecG -
  NGC85_RS15130 (NGC85_15130) tpiA 3198102..3198893 (-) 792 WP_254732214.1 triose-phosphate isomerase -
  NGC85_RS15135 (NGC85_15135) pilB 3199263..3200975 (+) 1713 WP_254732215.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGC85_RS15140 (NGC85_15140) pilC 3201000..3202226 (+) 1227 WP_254732216.1 type II secretion system F family protein Machinery gene
  NGC85_RS17450 pilD 3202226..3203086 (+) 861 WP_302885508.1 A24 family peptidase Machinery gene
  NGC85_RS15155 (NGC85_15155) coaE 3203090..3203695 (+) 606 WP_169108869.1 dephospho-CoA kinase -
  NGC85_RS15160 (NGC85_15160) - 3203692..3204603 (-) 912 WP_254732217.1 DMT family transporter -
  NGC85_RS15165 (NGC85_15165) rlmB 3204634..3205383 (-) 750 WP_254732218.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NGC85_RS15170 (NGC85_15170) - 3205573..3205899 (-) 327 WP_005050993.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63152.29 Da        Isoelectric Point: 5.0067

>NTDB_id=708748 NGC85_RS15135 WP_254732215.1 3199263..3200975(+) (pilB) [Acinetobacter sp. Z1]
MSGLHTSPKFSGFIRRLVEDGHMTASNMQNAIDAAKKAHQDIVAHLIEHHRLAPLTIAESISVEFGEPLFDLGVYDTALL
PRELTDNKLIQKHRVVPLLQRGQILYVATSNPTNIDALDAIRFNTKLNIEAVIVEFNKLDKLIEQQFGDSSSFDFGDSED
FDLDVDVGTEAPSEDDDNTQSDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMANRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCAQCKIPTDVPKQSLLELGF
TEQDLADPELTIYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNAIEIASASERAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=708748 NGC85_RS15135 WP_254732215.1 3199263..3200975(+) (pilB) [Acinetobacter sp. Z1]
ATGTCAGGATTACATACGTCTCCAAAATTTTCTGGGTTTATTCGACGTTTAGTTGAAGATGGTCACATGACTGCATCAAA
TATGCAAAATGCAATTGATGCAGCAAAAAAGGCTCACCAAGATATTGTTGCTCATTTAATAGAACATCATCGTCTTGCTC
CGCTTACCATTGCGGAAAGTATCTCTGTCGAGTTTGGAGAACCACTATTTGATTTAGGGGTTTACGATACTGCTTTACTA
CCACGTGAGTTAACCGATAACAAACTCATTCAGAAACATCGTGTGGTGCCTTTGCTTCAGCGTGGACAGATTTTGTATGT
CGCTACCAGCAATCCCACCAATATTGATGCATTAGATGCGATTCGCTTTAATACAAAACTCAACATAGAAGCTGTCATTG
TCGAATTCAATAAATTAGACAAACTGATTGAACAACAGTTTGGTGATAGTTCAAGTTTTGATTTTGGTGATAGTGAAGAT
TTTGATCTAGATGTTGATGTCGGTACAGAAGCTCCATCAGAAGATGATGATAATACACAAAGTGATGAGTCACCGATTGT
AAAATATATCAACAAACTCTTGATTGATGCGATTCGTATGGGCGCATCAGACTTACATTTTGAACCTTATGAAAAAATGT
ATCGTGTCCGCTATCGCGTCGATGGCGTTTTAAGACAAATTGCAAATCCACCCTTGCAGATGGCAAATCGATTAGCATCG
CGTTTAAAAGTAATGTCGCAAATGGATATTTCTGAAAAGCGAATGCCTCAAGATGGTCGTATTAAGTTAAAGCTATCTAA
AACCAAAGCCATTGATTTCCGTGTCAATTCGCTACCCACCCTGTTTGGTGAGAAACTCGTTTTACGTATTCTAGATCCAT
CCAGTGCCATGCTCGGAATTGAGGCTTTAGGCTATGAGGATGAGCAAAAAGCACTATTTTTAGAAGCACTTGATAAGCCA
CAAGGCATGCTACTAATTACAGGCCCAACTGGTTCAGGTAAAACTGTATCTTTATATACTGGTTTGAATATTCTCAATAC
TGAAGATACCAATATTTCAACTGCCGAAGACCCAGTTGAAATCAACTTGGAAGGGATTAACCAAGTCAACGTGAATCCCA
AAACAGGACTTACCTTCTCTGCTGCATTGAAAGCATTCTTACGCCAAGATCCCGACATTATCATGGTTGGTGAGATTCGT
GACCTTGAAACGGCTGAAATTGCCATCAAGGCTGCGCAAACAGGACATATGGTGATGTCAACACTCCATACCAACAGTGC
TCCTGAAACATTAACACGTTTACGTAATATGGGGGTTCCTTCATTTAATATTGCAACGTCTGTCAACTTAGTGATTGCTC
AACGTTTAGCACGTCGTTTATGCGCACAATGTAAAATACCAACAGATGTTCCTAAACAAAGTCTTTTAGAACTTGGTTTC
ACAGAACAAGATTTAGCAGATCCAGAACTCACAATTTATCAACCTGTTGGCTGTGCTGAATGTCGAGAAGGTTATAAAGG
ACGTGTTGGTATTTATGAAGTAATGAAAGTGACACCAGAAATCTCAAAGATCATCATGGAAGATGGCAATGCCATAGAGA
TTGCTTCTGCATCGGAAAGAGCAGGCTTTAATAACCTACGTCGCTCGGGTCTGAAAAAAGTGATGCAAGGAATAACTTCT
TTGCAAGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.137

100

0.823
  pilB Acinetobacter baylyi ADP1

76.182

100

0.763
  pilB Legionella pneumophila strain ERS1305867

54.019

93.86

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.358

97.895

0.493
  pilF Neisseria gonorrhoeae MS11

49.12

99.649

0.489
  pilB Vibrio campbellii strain DS40M4

49.464

98.246

0.486
  pilB Vibrio cholerae strain A1552

50.87

90.702

0.461
  pilF Thermus thermophilus HB27

38.475

98.947

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

34.314

100

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

92.982

0.365