Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NLL86_RS01680 Genome accession   NZ_CP100660
Coordinates   379196..380443 (+) Length   415 a.a.
NCBI ID   WP_254485514.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain ZL22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374196..385443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLL86_RS01660 (NLL86_01660) - 374218..376671 (-) 2454 WP_254482663.1 penicillin-binding protein 1A -
  NLL86_RS01665 (NLL86_01665) pilM 376848..377699 (+) 852 WP_254482664.1 type IV pilus biogenesis protein PilM -
  NLL86_RS01670 (NLL86_01670) - 377699..378235 (+) 537 WP_254482665.1 PilN domain-containing protein -
  NLL86_RS01675 (NLL86_01675) - 378232..379251 (+) 1020 WP_254482666.1 pilus assembly protein PilP -
  NLL86_RS01680 (NLL86_01680) pilQ 379196..380443 (+) 1248 WP_254485514.1 type IV pilus secretin PilQ Machinery gene
  NLL86_RS01685 (NLL86_01685) aroK 380448..380966 (+) 519 WP_054884228.1 shikimate kinase AroK -
  NLL86_RS01690 (NLL86_01690) aroB 381060..382157 (+) 1098 WP_254482667.1 3-dehydroquinate synthase -
  NLL86_RS01695 (NLL86_01695) - 382173..383792 (+) 1620 WP_254482668.1 AAA family ATPase -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 44952.44 Da        Isoelectric Point: 4.7227

>NTDB_id=706106 NLL86_RS01680 WP_254485514.1 379196..380443(+) (pilQ) [Pseudomonas fluorescens strain ZL22]
MTVALLTSQLCMAAPYRGEPISLNFQDVEVRSVLQVLADYAGINLVASDTVQGNVTLRLHEVPWDQALALVLRSKGLARR
EEGNVLLVAPAAELAEQSRGARIGQELDAQLQPLQRELLPIHHAQAADLAELLLATLADDGILTGRGSLSVDARTNTLVA
YQPADRLADLRQLVAQLDVPVRQVEIEARIVEANVDYEKGLGVRWGRQLYGEAPQLGKDLFVDLGMERGGAGIGVGLLRG
GVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVAFREASLSLEVTPQITPDGKVIMTVK
VTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVF
LTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=706106 NLL86_RS01680 WP_254485514.1 379196..380443(+) (pilQ) [Pseudomonas fluorescens strain ZL22]
ATGACGGTGGCGCTTCTCACCAGTCAGTTGTGCATGGCAGCGCCCTATCGGGGTGAGCCCATCTCGCTGAATTTTCAGGA
TGTCGAGGTGCGCTCGGTGCTTCAAGTGCTGGCGGACTACGCCGGGATCAACCTGGTGGCGAGCGATACCGTGCAGGGCA
ATGTTACCCTGCGGTTGCACGAAGTCCCGTGGGACCAGGCGCTGGCGCTGGTGCTACGTAGCAAAGGGCTGGCCCGGCGC
GAGGAAGGGAATGTGCTGCTGGTGGCGCCAGCCGCCGAGTTGGCCGAACAATCCCGCGGTGCCCGTATCGGGCAAGAGCT
GGATGCGCAGCTGCAACCCTTGCAGCGTGAGCTGCTGCCGATCCATCACGCCCAGGCGGCCGACCTCGCCGAGTTGTTGC
TGGCAACCCTGGCGGACGATGGCATCCTGACCGGTCGTGGCAGCCTGAGCGTCGACGCTCGTACCAACACCTTGGTCGCG
TACCAGCCTGCGGATCGCCTTGCCGATCTGCGGCAGTTGGTCGCTCAGCTGGACGTGCCGGTGCGGCAGGTGGAGATCGA
GGCGCGTATCGTCGAGGCCAATGTCGATTACGAGAAAGGCCTGGGCGTGCGCTGGGGGCGGCAGTTGTACGGGGAGGCGC
CGCAACTGGGCAAGGACCTGTTTGTCGATCTGGGCATGGAGCGGGGCGGTGCTGGCATCGGCGTAGGCCTGCTGCGTGGC
GGCGTGCTGCTGGACCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAAATCATCTCCCAGCCCAAGGTGGTCAC
GGCGGACAAGGAAACCGCCAGGATTCTCAAGGGCACCGAGGTGCCTTATCAGGAAACCAGCCAGAGTGGCGCCACCTCGG
TGGCCTTTCGCGAGGCGTCGTTGTCGCTGGAGGTCACGCCGCAGATCACCCCGGACGGCAAGGTCATCATGACGGTGAAG
GTGACCAAGGACGAGCCTGACTTCGTCAATGCCCTGAACGACGTGCCGCCGATTCGCAAGAATGAGGTCAATGCCAAGGT
TCGCGTGGCGGACGGTGAAACCATCGTCATCGGGGGTGTCTACTCGACCACGCAAAACAATGTGGTCGACAAGGTGCCAT
TTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTC
CTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(250-406)

Secretin and TonB N terminus short domain (STN)

(44-90)

Bacterial type II/III secretion system short domain (Secretin_N)

(118-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.945

100

0.619
  comQ Acinetobacter baylyi ADP1

43.267

100

0.472
  pilQ Acinetobacter baumannii strain A118

40.558

100

0.455
  pilQ Acinetobacter baumannii D1279779

40.558

100

0.455
  pilQ Legionella pneumophila strain ERS1305867

42.823

100

0.431
  pilQ Legionella pneumophila strain Lp02

42.823

100

0.431
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.687

100

0.417
  pilQ Vibrio cholerae strain A1552

41.687

100

0.417
  pilQ Vibrio campbellii strain DS40M4

40.426

100

0.412