Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NLY38_RS03895 Genome accession   NZ_CP100553
Coordinates   843593..845296 (-) Length   567 a.a.
NCBI ID   WP_195881052.1    Uniprot ID   -
Organism   Pseudomonas hydrolytica strain KHPS2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 838593..850296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLY38_RS03860 (NLY38_03860) - 838715..839344 (-) 630 WP_003242718.1 DUF1780 domain-containing protein -
  NLY38_RS03865 (NLY38_03865) - 839471..840160 (+) 690 WP_195881056.1 energy-coupling factor ABC transporter permease -
  NLY38_RS03870 (NLY38_03870) - 840269..840484 (+) 216 WP_096827395.1 hypothetical protein -
  NLY38_RS03875 (NLY38_03875) yacG 840536..840730 (-) 195 WP_003242724.1 DNA gyrase inhibitor YacG -
  NLY38_RS03880 (NLY38_03880) coaE 840727..841335 (-) 609 WP_195881055.1 dephospho-CoA kinase -
  NLY38_RS03885 (NLY38_03885) pilD 841503..842372 (-) 870 WP_195881054.1 A24 family peptidase Machinery gene
  NLY38_RS03890 (NLY38_03890) pilC 842373..843590 (-) 1218 WP_195881053.1 type II secretion system F family protein Machinery gene
  NLY38_RS03895 (NLY38_03895) pilB 843593..845296 (-) 1704 WP_195881052.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NLY38_RS03900 (NLY38_03900) - 845520..845993 (+) 474 WP_195881051.1 pilin -
  NLY38_RS03910 (NLY38_03910) - 846093..846503 (+) 411 WP_302055162.1 pilin -
  NLY38_RS03915 (NLY38_03915) nadC 846722..847570 (-) 849 WP_195881049.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62375.40 Da        Isoelectric Point: 5.3482

>NTDB_id=705821 NLY38_RS03895 WP_195881052.1 843593..845296(-) (pilB) [Pseudomonas hydrolytica strain KHPS2]
MNESVSLSGLAKQMVLAELLDEKTAQQAQLQAQRNKLSLVTYLVQNKLVKSRALAELAAEQFGVAFFDLNAIDKEGQPRE
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAVTDVQFSTGLNTEAILVEDDKLGDAIDKFFDSANSGMEDLADVDLD
GLDVEAVDDDKPAGEGGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGLLHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEVNVPRETLLAEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=705821 NLY38_RS03895 WP_195881052.1 843593..845296(-) (pilB) [Pseudomonas hydrolytica strain KHPS2]
ATGAACGAAAGCGTATCCCTCTCCGGCCTGGCCAAGCAGATGGTATTGGCCGAGCTGCTGGACGAAAAAACCGCTCAACA
AGCGCAACTGCAGGCCCAGCGCAACAAGCTGTCGCTGGTTACCTACCTGGTACAGAACAAGCTGGTAAAGAGCCGCGCCC
TGGCCGAATTGGCCGCTGAGCAGTTCGGCGTGGCTTTTTTTGACCTTAACGCCATCGATAAAGAAGGGCAGCCCAGGGAG
CTGGTCAGCGAGAAGTTGGTGCGCCAGCACCGCGTGCTGCCGCTCTGGCGACGTGGTAACAAGCTGTTCGTCGGGCTCTC
CGATCCGACCAACCACCAGGCGGTAACCGATGTGCAGTTCAGCACCGGCCTCAATACCGAGGCGATTCTGGTCGAGGACG
ACAAACTCGGCGATGCCATCGACAAGTTCTTCGACTCGGCCAATAGCGGCATGGAGGATCTGGCCGACGTCGACCTGGAT
GGTTTGGACGTCGAGGCAGTGGATGACGACAAACCCGCCGGAGAAGGCGGTGCGGACGCCGATGACGCGCCAGTCGTACG
CTTCGTCAACAAGATGCTACTGGACGCCATCAAAGGCGGCTCATCCGACCTGCACTTTGAACCTTACGAAAAGGCCTACC
GGGTACGCTTTCGTACCGACGGCCTGCTGCATGAGGTTGCCCGGCCACCTATCCAGCTTGCGCCACGTATTTCCGCCCGC
CTCAAGGTCATGGCTGCACTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAAATTTCCAAGAC
CAAGGCCATCGACTTTCGCGTCAACACCTTGCCTACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCACCA
GCGCACAGATGGGCATTGATGCCCTGGGTTACGAGGAAGAACAGAAAGAGCTCTACCTAAGCGCTCTGAAACAACCGCAG
GGAATGATTCTGGTCACCGGCCCTACGGGTTCGGGCAAAACCGTATCGCTCTATACCGGCCTGAATATTCTCAATACCCC
GGATGTTAATATCTCTACCGCCGAAGACCCTGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCCAAAC
AGGGCATGGACTTTACCCAGGCGCTGCGTGCTTTTCTGCGTCAGGACCCGGATATCATCATGGTCGGTGAGATCCGTGAC
CTGGATACGGCGTCCATTGCCGTCAAGGCCGCACAGACCGGCCATATGGTCATGTCCACCCTGCACACCAATAGCGCCGC
GGAAACCCTGACCCGCCTGCGCAATATGGGTGTGCCGTCCTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTTTGCAACAGCTGCAAGAAAGAAGTGAACGTACCGCGTGAAACCCTGCTGGCCGAGGGTTTCCCT
GAGGACAAGATCGGCACCTTCAAGATCTATGGCCCGGTAGGCTGCGAAAATTGCAAGGGCGGTTACAAGGGCCGAGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AGATGCGTAAAGACGGCTTTAATGACCTACGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTTACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.766

100

0.584
  pilB Acinetobacter baylyi ADP1

57.726

99.295

0.573
  pilB Legionella pneumophila strain ERS1305867

55.185

100

0.554
  pilB Vibrio cholerae strain A1552

48.759

99.471

0.485
  pilF Neisseria gonorrhoeae MS11

48.49

99.295

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.358

96.12

0.474
  pilB Vibrio campbellii strain DS40M4

48.535

96.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

35.541

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.651

0.376
  pilF Thermus thermophilus HB27

37.168

99.647

0.37