Detailed information NTG0705080

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF364_RS04450
• Genome accession: NZ_CP115621
• Coordinates: 931188..932306 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain 2022CK-00480
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 926188..937306

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF364_RS04420 (PF364_04420)	-	927017..927865 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
PF364_RS04425 (PF364_04425)	-	928118..928573 (+)	456	WP_000782976.1	bacteriohemerythrin	-
PF364_RS04430 (PF364_04430)	-	928726..929793 (+)	1068	WP_031959810.1	membrane protein	-
PF364_RS04435 (PF364_04435)	-	929793..930116 (+)	324	WP_000442585.1	RnfH family protein	-
PF364_RS04440 (PF364_04440)	bamE	930171..930569 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF364_RS04445 (PF364_04445)	fur	930681..931118 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF364_RS04450 (PF364_04450)	pilU	931188..932306 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF364_RS04455 (PF364_04455)	pilT	932334..933371 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF364_RS04460 (PF364_04460)	-	933498..934190 (+)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=705080 PF364_RS04450 WP_000347036.1 931188..932306(-) (pilU) [Acinetobacter baumannii strain 2022CK-00480]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=705080 PF364_RS04450 WP_000347036.1 931188..932306(-) (pilU) [Acinetobacter baumannii strain 2022CK-00480]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392