Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NIN90_RS12870 Genome accession   NZ_CP100422
Coordinates   2784743..2786431 (+) Length   562 a.a.
NCBI ID   WP_232658179.1    Uniprot ID   -
Organism   Vibrio furnissii strain 104486766     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779743..2791431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIN90_RS12840 dsbC 2780062..2780826 (-) 765 WP_158107763.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  NIN90_RS12845 xerD 2780843..2781751 (-) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  NIN90_RS12850 fldB 2781893..2782414 (+) 522 WP_055466520.1 flavodoxin FldB -
  NIN90_RS12855 ampD 2782450..2783004 (-) 555 WP_232658178.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NIN90_RS12860 nadC 2783153..2784040 (+) 888 WP_055466518.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NIN90_RS12865 - 2784287..2784736 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NIN90_RS12870 pilB 2784743..2786431 (+) 1689 WP_232658179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NIN90_RS12875 pilC 2786510..2787736 (+) 1227 WP_055466515.1 type II secretion system F family protein Machinery gene
  NIN90_RS12880 pilD 2787788..2788657 (+) 870 WP_004729087.1 A24 family peptidase Machinery gene
  NIN90_RS12885 coaE 2788660..2789268 (+) 609 WP_014204457.1 dephospho-CoA kinase -
  NIN90_RS12890 zapD 2789301..2790041 (+) 741 WP_004729090.1 cell division protein ZapD -
  NIN90_RS12895 yacG 2790099..2790296 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62092.21 Da        Isoelectric Point: 6.1994

>NTDB_id=705079 NIN90_RS12870 WP_232658179.1 2784743..2786431(+) (pilB) [Vibrio furnissii strain 104486766]
MLTNLIAILRQAKLISPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQFYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=705079 NIN90_RS12870 WP_232658179.1 2784743..2786431(+) (pilB) [Vibrio furnissii strain 104486766]
ATGCTTACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATCGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTAA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCATAAAGCGCTGCACGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATTGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTAGATATTGCCGAGCGCCGTCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATCGTTCTGCGCCTACTCGACAGTAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAACAGAAACAGTTCTACCTCGCAGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGAGAAATCCGCGATCTAGAAACC
GCTGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCGGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACAGAATGCACCAATGGGTATAGCGGCCGCGTGGGAATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTGCAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.206

100

0.822
  pilB Vibrio campbellii strain DS40M4

71.708

100

0.717
  pilB Vibrio parahaemolyticus RIMD 2210633

71.708

100

0.717
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

50.867

92.349

0.47
  pilB Acinetobacter baylyi ADP1

49.903

91.993

0.459
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367