Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NKT35_RS04885 Genome accession   NZ_CP100128
Coordinates   1050327..1050782 (-) Length   151 a.a.
NCBI ID   WP_305883467.1    Uniprot ID   -
Organism   Chromobacterium sp. IIBBL 290-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045327..1055782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKT35_RS04870 (NKT35_04870) rpoH 1045538..1046401 (+) 864 WP_254299367.1 RNA polymerase sigma factor RpoH -
  NKT35_RS04875 (NKT35_04875) - 1046488..1048272 (-) 1785 WP_254299369.1 PglL family O-oligosaccharyltransferase -
  NKT35_RS04880 (NKT35_04880) - 1048365..1050176 (-) 1812 WP_254299371.1 PglL family O-oligosaccharyltransferase -
  NKT35_RS04885 (NKT35_04885) pilA 1050327..1050782 (-) 456 WP_305883467.1 pilin Machinery gene
  NKT35_RS04895 (NKT35_04895) - 1051036..1052385 (+) 1350 WP_254299373.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  NKT35_RS04900 (NKT35_04900) - 1052476..1053978 (+) 1503 WP_254299375.1 hypothetical protein -
  NKT35_RS04905 (NKT35_04905) proB 1054054..1055172 (+) 1119 WP_254299377.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15422.54 Da        Isoelectric Point: 8.4401

>NTDB_id=703704 NKT35_RS04885 WP_305883467.1 1050327..1050782(-) (pilA) [Chromobacterium sp. IIBBL 290-4]
MKTRKQQGFTLIELMIVVAIVGILAAIAIPTYQDYTKRAKVTEGLSLADAAKTAVTEYYSTNNGFAAAGNSNYGLAASIT
GSSVSGVNVLASGVIQITYTSAVSSGALLDIVPTAASGSLTWTCTYLGTGATAIASGNQLSASWVPTTCRQ

Nucleotide


Download         Length: 456 bp        

>NTDB_id=703704 NKT35_RS04885 WP_305883467.1 1050327..1050782(-) (pilA) [Chromobacterium sp. IIBBL 290-4]
ATGAAGACGAGAAAACAGCAGGGTTTTACCTTGATCGAGCTGATGATCGTGGTGGCGATTGTCGGCATTCTGGCGGCCAT
CGCGATTCCGACTTATCAGGACTACACCAAGCGCGCGAAGGTAACGGAAGGTTTGTCCCTGGCGGATGCGGCCAAGACGG
CGGTAACCGAGTATTACTCCACCAATAACGGCTTTGCCGCCGCAGGCAACTCCAATTACGGGCTTGCCGCCTCCATTACG
GGCTCTTCCGTATCCGGGGTGAATGTATTGGCCAGCGGCGTGATTCAAATCACCTATACTTCCGCTGTGTCAAGCGGCGC
CTTGCTGGATATCGTGCCAACCGCCGCTTCCGGTTCATTGACCTGGACCTGCACTTATCTCGGCACCGGCGCCACCGCGA
TCGCATCCGGGAATCAGCTTAGCGCAAGCTGGGTGCCGACCACCTGCCGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(38-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

44.848

100

0.49
  pilA2 Legionella pneumophila strain ERS1305867

49.315

96.689

0.477
  pilA2 Legionella pneumophila str. Paris

49.315

96.689

0.477
  pilE Neisseria gonorrhoeae strain FA1090

42.038

100

0.437
  pilE Neisseria gonorrhoeae MS11

41.401

100

0.43
  comP Acinetobacter baylyi ADP1

40.123

100

0.43
  pilA/pilA1 Eikenella corrodens VA1

38.509

100

0.411
  pilA Haemophilus influenzae 86-028NP

43.939

87.417

0.384