Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NI383_RS20450 Genome accession   NZ_CP099955
Coordinates   2673870..2675093 (+) Length   407 a.a.
NCBI ID   WP_315868305.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain Isc4A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2668870..2680093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI383_RS20430 (NI383_20585) ampD 2669913..2670464 (-) 552 WP_140284224.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI383_RS20435 (NI383_20590) nadC 2670557..2671444 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI383_RS20440 (NI383_20595) - 2671707..2672159 (+) 453 WP_029846089.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NI383_RS20445 (NI383_20600) pilB 2672161..2673846 (+) 1686 WP_315868304.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI383_RS20450 (NI383_20605) pilC 2673870..2675093 (+) 1224 WP_315868305.1 type II secretion system F family protein Machinery gene
  NI383_RS20455 (NI383_20610) pilD 2675158..2676027 (+) 870 WP_140154970.1 A24 family peptidase Machinery gene
  NI383_RS20460 (NI383_20615) coaE 2676028..2676642 (+) 615 WP_005480887.1 dephospho-CoA kinase -
  NI383_RS20465 (NI383_20620) zapD 2676670..2677410 (+) 741 WP_015297254.1 cell division protein ZapD -
  NI383_RS20470 (NI383_20625) yacG 2677479..2677673 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  NI383_RS20475 (NI383_20630) rplS 2678081..2678434 (-) 354 WP_005462554.1 50S ribosomal protein L19 -
  NI383_RS20480 (NI383_20635) trmD 2678476..2679219 (-) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  NI383_RS20485 (NI383_20640) rimM 2679247..2679795 (-) 549 WP_005462552.1 ribosome maturation factor RimM -
  NI383_RS20490 (NI383_20645) rpsP 2679824..2680072 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45242.39 Da        Isoelectric Point: 10.2879

>NTDB_id=702893 NI383_RS20450 WP_315868305.1 2673870..2675093(+) (pilC) [Vibrio parahaemolyticus strain Isc4A]
MKITTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMHTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLELSAWTQNWSPFIALGSISLFISA
RVLSERSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=702893 NI383_RS20450 WP_315868305.1 2673870..2675093(+) (pilC) [Vibrio parahaemolyticus strain Isc4A]
ATGAAAATTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAAGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCATACCGCCAGCAACCATTTTGACCCGCTAT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCTACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTGGGCTTTGGTGCTGAATTGCCGTGGTTTACAAGGC
AAGTCTTAGAGCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGTTCCATCAGTTTATTTATCTCCGCG
AGAGTACTCTCTGAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAGTTTCCAGTCCTTGGGGCTGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACCCTCGCTACGAGCTTTACGGCGGGCATTCCGATTTTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTCGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTGGAACCATTAATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTAGTCACCGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

42.5

98.28

0.418
  pilC Legionella pneumophila strain ERS1305867

40.244

100

0.405
  pilC Acinetobacter baumannii D1279779

40.943

99.017

0.405
  pilG Neisseria gonorrhoeae MS11

40.247

99.509

0.4
  pilG Neisseria meningitidis 44/76-A

40

99.509

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.657

97.297

0.396