Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NI391_RS12230 Genome accession   NZ_CP099951
Coordinates   2699197..2700420 (+) Length   407 a.a.
NCBI ID   WP_017635918.1    Uniprot ID   A0AAW9BMM9
Organism   Vibrio alginolyticus strain Isc25B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2694197..2705420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI391_RS12210 (NI391_12220) ampD 2695245..2695796 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI391_RS12215 (NI391_12225) nadC 2695889..2696776 (+) 888 WP_064354288.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI391_RS12220 (NI391_12230) - 2697038..2697481 (+) 444 WP_064354289.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NI391_RS12225 (NI391_12235) pilB 2697488..2699173 (+) 1686 WP_064354290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI391_RS12230 (NI391_12240) pilC 2699197..2700420 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  NI391_RS12235 (NI391_12245) pilD 2700485..2701354 (+) 870 WP_064354291.1 A24 family peptidase Machinery gene
  NI391_RS12240 (NI391_12250) coaE 2701355..2701969 (+) 615 WP_064354292.1 dephospho-CoA kinase -
  NI391_RS12245 (NI391_12255) zapD 2701997..2702737 (+) 741 WP_005373086.1 cell division protein ZapD -
  NI391_RS12250 (NI391_12260) yacG 2702896..2703090 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  NI391_RS12255 (NI391_12265) rplS 2703574..2703927 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  NI391_RS12260 (NI391_12270) trmD 2703969..2704730 (-) 762 WP_064354293.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  NI391_RS12265 (NI391_12275) rimM 2704758..2705306 (-) 549 WP_005379964.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45313.50 Da        Isoelectric Point: 10.3992

>NTDB_id=702844 NI391_RS12230 WP_017635918.1 2699197..2700420(+) (pilC) [Vibrio alginolyticus strain Isc25B]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=702844 NI391_RS12230 WP_017635918.1 2699197..2700420(+) (pilC) [Vibrio alginolyticus strain Isc25B]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATTACGGTGTTCACCCGTCAGATTTCGACGATGTTG
ATAACTGGTGTGCCCTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAACCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGTACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGACCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTATTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCCCTGATTTACCCAGCCATGGTAGTGCTAGTAGCCTTAGGCGTCTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCTGCTATCGCCAAATTTAGTCGAACACTCGCAACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGATAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCGCTAATTATCGTTTTTTTAGGCATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398