Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NI392_RS20650 Genome accession   NZ_CP099950
Coordinates   2711349..2712572 (+) Length   407 a.a.
NCBI ID   WP_047102659.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc1B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2706349..2717572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI392_RS20630 (NI392_20785) ampD 2707409..2707960 (-) 552 WP_053304127.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI392_RS20635 (NI392_20790) nadC 2708053..2708940 (+) 888 WP_104975579.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI392_RS20640 (NI392_20795) - 2709203..2709643 (+) 441 WP_017447682.1 pilin -
  NI392_RS20645 (NI392_20800) pilB 2709640..2711325 (+) 1686 WP_053304129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI392_RS20650 (NI392_20805) pilC 2711349..2712572 (+) 1224 WP_047102659.1 type II secretion system F family protein Machinery gene
  NI392_RS20655 (NI392_20810) pilD 2712637..2713506 (+) 870 WP_017635919.1 A24 family peptidase Machinery gene
  NI392_RS20660 (NI392_20815) coaE 2713507..2714121 (+) 615 WP_065274841.1 dephospho-CoA kinase -
  NI392_RS20665 (NI392_20820) zapD 2714149..2714889 (+) 741 WP_005373086.1 cell division protein ZapD -
  NI392_RS20670 (NI392_20825) yacG 2715048..2715242 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  NI392_RS20675 (NI392_20830) rplS 2716069..2716422 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  NI392_RS20680 (NI392_20835) trmD 2716464..2717225 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45278.41 Da        Isoelectric Point: 10.3992

>NTDB_id=702804 NI392_RS20650 WP_047102659.1 2711349..2712572(+) (pilC) [Vibrio alginolyticus strain Isc1B]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENNKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFTSA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=702804 NI392_RS20650 WP_047102659.1 2711349..2712572(+) (pilC) [Vibrio alginolyticus strain Isc1B]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTCACCCGTCAGATTTCGACGATGTTG
ATAACTGGTGTGCCGTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAACAATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCCGGTACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGACCTTATCGCAACAGGTGAGCAATCCGGTAACTTAGCGGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAGCAGCTTCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCCATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCACCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCCATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGACATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGATAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.732

100

0.867
  pilC Vibrio cholerae strain A1552

73.529

100

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Legionella pneumophila strain ERS1305867

40.732

100

0.41
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

39.655

99.754

0.396