Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NI380_RS03215 Genome accession   NZ_CP099934
Coordinates   657980..658384 (-) Length   134 a.a.
NCBI ID   WP_015297250.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain BM26A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652980..663384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI380_RS03195 (NI380_03190) coaE 653500..654114 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  NI380_RS03200 (NI380_03195) pilD 654115..654984 (-) 870 WP_308386333.1 A24 family peptidase Machinery gene
  NI380_RS03205 (NI380_03200) - 655049..656271 (-) 1223 Protein_551 type II secretion system F family protein -
  NI380_RS03210 (NI380_03205) pilB 656295..657980 (-) 1686 WP_020835398.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI380_RS03215 (NI380_03210) pilA 657980..658384 (-) 405 WP_015297250.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NI380_RS03220 (NI380_03215) nadC 658647..659534 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI380_RS03225 (NI380_03220) ampD 659627..660178 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI380_RS03230 (NI380_03225) pdhR 660584..661351 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13659.55 Da        Isoelectric Point: 6.4433

>NTDB_id=702725 NI380_RS03215 WP_015297250.1 657980..658384(-) (pilA) [Vibrio parahaemolyticus strain BM26A]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYQNYVAKSEAATALGSLRALVTPAELKLQQDGELSGVVADLGGSAS
HALGAITTSGANISAATLTFTFNTGSLSGDAITLTKTSSGWTCTDGTTVLDNCN

Nucleotide


Download         Length: 405 bp        

>NTDB_id=702725 NI380_RS03215 WP_015297250.1 657980..658384(-) (pilA) [Vibrio parahaemolyticus strain BM26A]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTGGCGATTATTGGGGTGTT
GTCAGCAATTGCAGTACCTGCTTATCAAAATTATGTCGCTAAAAGTGAAGCGGCAACGGCATTAGGAAGTTTGAGAGCAT
TAGTCACTCCTGCTGAACTTAAACTTCAACAAGATGGAGAGCTATCTGGTGTAGTAGCTGACTTAGGTGGATCTGCATCA
CATGCATTAGGTGCTATTACAACATCTGGTGCTAACATAAGCGCTGCTACTCTAACCTTCACGTTTAATACAGGCTCATT
AAGTGGTGATGCTATCACACTAACCAAAACAAGTTCTGGTTGGACATGTACTGATGGCACTACGGTTTTAGACAACTGTA
ACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-130)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.143

100

0.545
  pilA Pseudomonas aeruginosa PAK

42.446

100

0.44
  pilA Vibrio cholerae strain A1552

42.029

100

0.433
  pilA Vibrio cholerae C6706

42.029

100

0.433
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.029

100

0.433
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.188

95.522

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.535

96.269

0.381