Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NI390_RS13190 Genome accession   NZ_CP099915
Coordinates   2806126..2807862 (-) Length   578 a.a.
NCBI ID   WP_024374092.1    Uniprot ID   -
Organism   Vibrio fluvialis strain Isc7A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2801126..2812862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI390_RS13170 (NI390_13165) - 2801829..2802658 (-) 830 Protein_2506 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NI390_RS13175 (NI390_13170) - 2802753..2804261 (-) 1509 WP_172570452.1 AAA family ATPase -
  NI390_RS13180 (NI390_13175) aroB 2804307..2805392 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  NI390_RS13185 (NI390_13180) aroK 2805420..2805938 (-) 519 WP_004728569.1 shikimate kinase AroK -
  NI390_RS13190 (NI390_13185) pilQ 2806126..2807862 (-) 1737 WP_024374092.1 type IV pilus secretin PilQ Machinery gene
  NI390_RS13195 (NI390_13190) pilP 2807887..2808399 (-) 513 WP_308413238.1 pilus assembly protein PilP Machinery gene
  NI390_RS13200 (NI390_13195) pilO 2808389..2808979 (-) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI390_RS13205 (NI390_13200) pilN 2808972..2809562 (-) 591 WP_024374094.1 PilN domain-containing protein Machinery gene
  NI390_RS13210 (NI390_13205) pilM 2809552..2810556 (-) 1005 WP_308413239.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62438.40 Da        Isoelectric Point: 4.5373

>NTDB_id=702496 NI390_RS13190 WP_024374092.1 2806126..2807862(-) (pilQ) [Vibrio fluvialis strain Isc7A]
MIKGLNASTAWGIRCLFVGVCLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=702496 NI390_RS13190 WP_024374092.1 2806126..2807862(-) (pilQ) [Vibrio fluvialis strain Isc7A]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACACTGGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGTATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGTCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTTAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAATGTTAACCTCGCAGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCCGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACCACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACTTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAGGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTACTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGCACTGGCGAGGCGGTGGC
GATCAACACGCAACGTATTGGTACACAGGTGCTGGTCAACAACGGTGAGACCGTGGTTCTGGGCGGCATTTTTCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCTGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.693

100

0.818
  pilQ Vibrio cholerae strain A1552

81.693

100

0.818
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382