Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI390_RS13200 Genome accession   NZ_CP099915
Coordinates   2808389..2808979 (-) Length   196 a.a.
NCBI ID   WP_020429291.1    Uniprot ID   A0AAX2LK04
Organism   Vibrio fluvialis strain Isc7A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2803389..2813979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI390_RS13180 (NI390_13175) aroB 2804307..2805392 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  NI390_RS13185 (NI390_13180) aroK 2805420..2805938 (-) 519 WP_004728569.1 shikimate kinase AroK -
  NI390_RS13190 (NI390_13185) pilQ 2806126..2807862 (-) 1737 WP_024374092.1 type IV pilus secretin PilQ Machinery gene
  NI390_RS13195 (NI390_13190) pilP 2807887..2808399 (-) 513 WP_308413238.1 pilus assembly protein PilP Machinery gene
  NI390_RS13200 (NI390_13195) pilO 2808389..2808979 (-) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI390_RS13205 (NI390_13200) pilN 2808972..2809562 (-) 591 WP_024374094.1 PilN domain-containing protein Machinery gene
  NI390_RS13210 (NI390_13205) pilM 2809552..2810556 (-) 1005 WP_308413239.1 type IV pilus assembly protein PilM Machinery gene
  NI390_RS13215 (NI390_13210) - 2810699..2813179 (+) 2481 WP_308413240.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22722.10 Da        Isoelectric Point: 4.7023

>NTDB_id=702498 NI390_RS13200 WP_020429291.1 2808389..2808979(-) (pilO) [Vibrio fluvialis strain Isc7A]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=702498 NI390_RS13200 WP_020429291.1 2808389..2808979(-) (pilO) [Vibrio fluvialis strain Isc7A]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCGCTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GCTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.592

100

0.796
  pilO Vibrio campbellii strain DS40M4

68.947

96.939

0.668