Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NI390_RS12150 Genome accession   NZ_CP099915
Coordinates   2596747..2597166 (+) Length   139 a.a.
NCBI ID   WP_222763982.1    Uniprot ID   -
Organism   Vibrio fluvialis strain Isc7A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2591747..2602166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI390_RS12125 (NI390_12120) dsbC 2592568..2593332 (-) 765 WP_024374397.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  NI390_RS12130 (NI390_12125) xerD 2593349..2594203 (-) 855 WP_308413471.1 site-specific tyrosine recombinase XerD -
  NI390_RS12135 (NI390_12130) fldB 2594398..2594919 (+) 522 WP_020430452.1 flavodoxin FldB -
  NI390_RS12140 (NI390_12135) ampD 2594924..2595475 (-) 552 WP_055452472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI390_RS12145 (NI390_12140) nadC 2595628..2596515 (+) 888 WP_308413212.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI390_RS12150 (NI390_12145) pilA 2596747..2597166 (+) 420 WP_222763982.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NI390_RS12155 (NI390_12150) pilB 2597245..2598862 (+) 1618 Protein_2312 type IV-A pilus assembly ATPase PilB -
  NI390_RS12160 (NI390_12155) pilC 2598898..2600124 (+) 1227 WP_222763978.1 type II secretion system F family protein Machinery gene
  NI390_RS12165 (NI390_12160) pilD 2600176..2601045 (+) 870 WP_222763976.1 A24 family peptidase Machinery gene
  NI390_RS12170 (NI390_12165) coaE 2601048..2601656 (+) 609 WP_222763974.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14284.25 Da        Isoelectric Point: 8.4642

>NTDB_id=702492 NI390_RS12150 WP_222763982.1 2596747..2597166(+) (pilA) [Vibrio fluvialis strain Isc7A]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVLTPAELYVQENGKISSGVSTITSLGV
SSAANSLGTLSISADNEITFSHTDGSAKGAKFVYTRTTGGWSCAYTAPAAVSAAAPKGC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=702492 NI390_RS12150 WP_222763982.1 2596747..2597166(+) (pilA) [Vibrio fluvialis strain Isc7A]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTCCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAGTTAGCTTCTGGCTTTGCCACAATTAAATCCG
TGCTAACACCAGCAGAGTTATATGTCCAAGAAAATGGAAAGATTAGTAGTGGAGTAAGTACCATCACATCGCTGGGTGTT
TCCTCTGCCGCTAATAGCTTAGGCACACTAAGTATCTCAGCAGACAATGAAATCACCTTTTCTCACACTGATGGTTCAGC
AAAGGGCGCGAAGTTCGTATACACAAGAACAACTGGTGGCTGGTCATGTGCGTACACCGCCCCAGCAGCCGTGAGCGCAG
CAGCACCGAAAGGTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.985

96.403

0.511
  pilA Acinetobacter baumannii strain A118

41.83

100

0.46
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.439
  pilA Vibrio cholerae C6706

37.662

100

0.417
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.662

100

0.417
  pilA Vibrio cholerae strain A1552

37.662

100

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.152

94.964

0.381