Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NHF51_RS18115 Genome accession   NZ_CP099717
Coordinates   3957920..3959626 (+) Length   568 a.a.
NCBI ID   WP_252995130.1    Uniprot ID   A0AAE9MGE3
Organism   Aeromonas encheleia strain SOD01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952920..3964626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHF51_RS18090 (NHF51_18090) pdhR 3954124..3954888 (-) 765 WP_218291524.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NHF51_RS18095 (NHF51_18095) ampD 3955165..3955737 (-) 573 WP_252995127.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NHF51_RS18100 (NHF51_18100) - 3955872..3956342 (+) 471 WP_042653204.1 TIGR02281 family clan AA aspartic protease -
  NHF51_RS18105 (NHF51_18105) nadC 3956346..3957203 (+) 858 WP_252995128.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NHF51_RS18110 (NHF51_18110) - 3957524..3957916 (+) 393 WP_252995129.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NHF51_RS18115 (NHF51_18115) pilB 3957920..3959626 (+) 1707 WP_252995130.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NHF51_RS18120 (NHF51_18120) pilC 3959757..3960998 (+) 1242 WP_252995131.1 type II secretion system F family protein Machinery gene
  NHF51_RS18125 (NHF51_18125) pilD 3961103..3961975 (+) 873 WP_252995132.1 A24 family peptidase Machinery gene
  NHF51_RS18130 (NHF51_18130) coaE 3961994..3962608 (+) 615 WP_252995133.1 dephospho-CoA kinase -
  NHF51_RS18135 (NHF51_18135) zapD 3962646..3963368 (+) 723 WP_218329451.1 cell division protein ZapD -
  NHF51_RS18140 (NHF51_18140) yacG 3963385..3963579 (+) 195 WP_042653212.1 DNA gyrase inhibitor YacG -
  NHF51_RS18145 (NHF51_18145) mutT 3963636..3964043 (-) 408 WP_252995134.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62544.88 Da        Isoelectric Point: 5.8786

>NTDB_id=701302 NHF51_RS18115 WP_252995130.1 3957920..3959626(+) (pilB) [Aeromonas encheleia strain SOD01]
MSSSPNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLTEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTTAIGKLMENEQDALGMDHIDESEI
SELEVSNEGSRLDEGVNTADDDAPIVKYINKIMLDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLKALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTNEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKTSEVVPDTELLELGFT
PQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRNLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=701302 NHF51_RS18115 WP_252995130.1 3957920..3959626(+) (pilB) [Aeromonas encheleia strain SOD01]
ATGTCCTCTAGCCCCAATAGCGGCCTGGCTACCAGCCTGGCCGCCTCTTCCCTTCTCAGTGAGCCAGACTCACAGCGTTA
TCTGAGCCAAGCAAAGGCGCAGCGCAAACCCTTTGTCACCTTCCTGATCGAGAACGATATCCTCGACAGCAAGGCGCTGG
CTGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGACCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGCCTGCACACGGAGGCCTTGTTGGTTGAAGAGAGCA
AACTGACCACTGCCATCGGCAAGCTGATGGAGAACGAGCAGGATGCGCTCGGCATGGACCATATCGACGAGTCCGAGATC
TCGGAACTCGAGGTCTCCAATGAAGGCTCACGACTCGATGAGGGAGTCAATACCGCGGATGACGATGCCCCGATCGTCAA
ATACATCAACAAAATCATGCTGGACGCCATCAAGCGCGGCGCCTCGGATCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGATCGACGGCATACTGCATGAGATAGCGACACCGCCCGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGATATTGCCGAACGACGCCTGCCGCAGGATGGCCGGATAAAGCTAAAAATATCGCGCAG
CAGGTCTATGGACATGAGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAAATCGTGATCCGCCTGCTGGACTCCTCGG
CGGCGCGACTCAATATAGAGCAACTCGGTTTCGACGACCGGCAGAAGGCCCAATACCTGAAAGCCCTCTCCAAACCCCAG
GGCATGATCCTGGTCACCGGCCCCACTGGATCCGGTAAAACGGTCTCACTCTATACCGGCCTCAACATTCTCAACACCAA
CGAGGTCAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGTGTCAATCAGGTGCAGATCAACCCCAAAG
CCGGTCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAAGATCCGGATATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCTCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACTCGGCCGC
CGAGACCCTGACGCGGATGATGAACATGGGGGTCCCCGCCTTCAACATCGCCTCCTCGGTGACTCTCATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGACGTCAGAGGTGGTGCCAGACACAGAGCTGCTGGAGCTGGGCTTTACC
CCCCAGCAACTGGCCGCCGGATTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGCTCGGGAGGATACAGAGGTCGCGT
CGGCATCTATGAAATCATGCTCATGTCCGACAATATCGCGAAGTTGATCATGCAGGGGGCCAACTCCCTGCAAATAGCCG
CCATTGCCCAGAAGGAGGGCATGCGTAACCTGCGTACCTCAGGTCTCGAGAAGGCTCGGCTGGGGGTCACCAGCCTGGCC
GAGATCAATCGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

100

0.551
  pilB Acinetobacter baylyi ADP1

55.24

99.12

0.548
  pilB Legionella pneumophila strain ERS1305867

54.77

99.648

0.546
  pilB Vibrio cholerae strain A1552

52.566

99.472

0.523
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.296

0.496
  pilF Neisseria gonorrhoeae MS11

45.745

99.296

0.454
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.613

97.359

0.366