Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NF677_RS12050 Genome accession   NZ_CP099599
Coordinates   2719160..2720269 (+) Length   369 a.a.
NCBI ID   WP_056785248.1    Uniprot ID   A0A5E7JTG7
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2714160..2725269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS12035 (NF677_12035) - 2715134..2716069 (+) 936 WP_252883712.1 sugar kinase -
  NF677_RS12040 (NF677_12040) - 2716185..2716460 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  NF677_RS12045 (NF677_12045) - 2716513..2719050 (-) 2538 WP_252885911.1 PAS domain-containing sensor histidine kinase -
  NF677_RS12050 (NF677_12050) pilU 2719160..2720269 (+) 1110 WP_056785248.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NF677_RS12055 (NF677_12055) - 2720412..2724101 (+) 3690 WP_252885912.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40872.81 Da        Isoelectric Point: 6.9929

>NTDB_id=700924 NF677_RS12050 WP_056785248.1 2719160..2720269(+) (pilU) [Pseudomonas siliginis strain OTU6ESPEB1]
MEIDALLSTLSQKNGSDLFLSTGAPPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKAFVSQRLVRTVDGQRRAAVEVLLGTPTVADALRRNELAELKSIMEKSEDSGMQTFDAAL
LQLVLARVITVEEALKNADSTNNLRLGIKLKLDSPINEKASTGDWGLVE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=700924 NF677_RS12050 WP_056785248.1 2719160..2720269(+) (pilU) [Pseudomonas siliginis strain OTU6ESPEB1]
ATGGAAATCGATGCACTGTTGTCAACATTGTCGCAAAAAAACGGTTCGGATCTCTTCCTTTCCACCGGCGCACCACCGAG
CGCTCGCATTGATGGTGTGCTGACGGCGCTCAGCGATCAGCCATTCAAACCTGGCGAAACCGCGGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGTGAGTTCGACCGGGATCTTGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGCCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAATGACGTTTCGATCGTTATCCGCAACGTCAAGCTCGACATCCCACGGTTCGA
AGACTTGAAACTGCCGCCGGTGTTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCTACCAGTT
CGGGCAAGTCGACCTCCCTGGCGGCGCTCATCGATCATCGCAACCGTCACAGTGCCGGACACATTGTTACCATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAGGTTGGCGTCGATACGCGCAGCTTTCATGC
TGCACTGAAGAACACTTTACGCCAGGCACCGGACGTCGTCCTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCACG
CGCTCTCGTTTGCCGATACAGGGCATCTGGTGTTGTCGACATTACATGCGACCAACGCCAATCAGGCGCTGGATCGCATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAGCAGCTGTTGCAGACATTGGGCAACAACCTGAAAGCATTTGTCTCGCAGCG
CTTGGTACGCACTGTCGACGGCCAGCGCAGGGCGGCCGTGGAAGTGTTATTAGGTACGCCGACTGTCGCTGATGCGCTCC
GTCGCAATGAGTTGGCTGAGTTGAAAAGTATCATGGAGAAATCCGAAGACTCAGGCATGCAGACATTCGATGCTGCACTG
TTACAGTTAGTCCTTGCGCGTGTTATTACAGTAGAAGAAGCTTTGAAAAACGCGGATTCGACAAACAACTTACGGTTAGG
CATCAAGCTTAAACTTGATTCTCCAATCAACGAAAAAGCATCGACAGGGGATTGGGGGTTGGTGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E7JTG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.125

95.122

0.534
  pilU Acinetobacter baylyi ADP1

52.841

95.393

0.504
  pilU Vibrio cholerae strain A1552

51.136

95.393

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

91.87

0.379
  pilT Vibrio cholerae strain A1552

40.24

90.244

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90.244

0.363
  pilT Neisseria gonorrhoeae MS11

38.329

94.038

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36