Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MN084_RS12475 Genome accession   NZ_CP099567
Coordinates   2259174..2260886 (+) Length   570 a.a.
NCBI ID   WP_241086176.1    Uniprot ID   -
Organism   Candidatus Vondammii sp. HM_W22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2254174..2265886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN084_RS12445 sucD 2254898..2255767 (-) 870 WP_241086181.1 succinate--CoA ligase subunit alpha -
  MN084_RS12450 sucC 2255792..2256955 (-) 1164 WP_241086180.1 ADP-forming succinate--CoA ligase subunit beta -
  MN084_RS12455 - 2257324..2257515 (-) 192 WP_241086179.1 hypothetical protein -
  MN084_RS12460 - 2257815..2257967 (-) 153 WP_241086178.1 hypothetical protein -
  MN084_RS12465 - 2258066..2258545 (-) 480 WP_241086177.1 pilin -
  MN084_RS12470 - 2258765..2258895 (-) 131 Protein_2455 transposase -
  MN084_RS12475 pilB 2259174..2260886 (+) 1713 WP_241086176.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MN084_RS12480 pilC 2260892..2262127 (+) 1236 WP_241086175.1 type II secretion system F family protein Machinery gene
  MN084_RS12485 pilD 2262218..2263090 (+) 873 WP_241086174.1 A24 family peptidase Machinery gene
  MN084_RS12490 coaE 2263096..2263695 (+) 600 WP_241086173.1 dephospho-CoA kinase -
  MN084_RS12495 zapD 2264179..2264475 (+) 297 WP_330178081.1 cell division protein ZapD -
  MN084_RS12500 - 2264634..2264834 (+) 201 WP_241086172.1 DNA gyrase inhibitor YacG -
  MN084_RS12505 - 2264902..2265843 (-) 942 WP_241086171.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63037.47 Da        Isoelectric Point: 6.0147

>NTDB_id=700708 MN084_RS12475 WP_241086176.1 2259174..2260886(+) (pilB) [Candidatus Vondammii sp. HM_W22]
MATTKPEVSVNGLARWLVHDSLLSEESAQQAFQEALKQRKPFVTHLVEKKMLKSREIAEYASQKYGIPLFDLDALDMETT
PSGLVEEKLVRTHHLLPLIKRGNRLFLGVSDPTNLQSLDEIKFHTGLSTEVILVEEDKLTKIIEIALQASETSISDMLAG
DDLDYLNITVEENEGVQDGDLDVDYAPVVRFVNKILLDGISTGASDIHFEHYEKTFRVRYRRDGILHEVASPPVSIASRL
TARIKVMSKLNVSERRVPQDGRIKMVLSRNRSIDFRVNTCPTLYGEKIVLRILDSTSTQLGIEALGLEPQQMNAFNAAIN
KPYGMILVTGPTGSGKTVSLYTALNILNKPEINISTVEDPVEIQVQGINQVNTNTKTGLTFASALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGIPAFSIASSVLLIMAQRLTRRLCQHCKEAEALPKEALLKE
GFKESELKDLTTYRAVGCDLCAQGYKGRLGIFQVMPISSNMEKLIMEGGNSMQLEALGKSEGTLDLRTSGLNKVRAGLTS
LEEINRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=700708 MN084_RS12475 WP_241086176.1 2259174..2260886(+) (pilB) [Candidatus Vondammii sp. HM_W22]
ATGGCAACAACCAAACCAGAAGTCAGTGTGAACGGATTGGCCCGCTGGCTGGTTCACGATAGCTTATTATCCGAAGAGAG
TGCCCAGCAGGCTTTTCAGGAGGCGCTAAAGCAGCGTAAACCGTTCGTTACCCATCTGGTTGAGAAAAAAATGCTTAAAA
GCAGGGAGATTGCCGAGTATGCCTCCCAGAAGTATGGTATTCCTCTGTTTGACCTGGATGCCCTTGATATGGAAACCACT
CCGTCAGGCCTGGTCGAAGAAAAACTGGTACGCACTCACCATCTCCTGCCTCTTATCAAACGTGGAAACCGACTGTTTCT
GGGAGTTTCTGATCCAACAAATCTACAAAGCCTTGATGAAATAAAATTCCATACCGGCCTCAGCACTGAAGTGATTCTAG
TCGAGGAAGATAAACTGACCAAAATCATTGAGATCGCACTTCAGGCTAGCGAAACCAGCATATCTGACATGCTGGCAGGT
GATGACCTGGATTATCTTAATATTACGGTTGAGGAAAACGAAGGGGTGCAGGATGGAGACCTGGATGTCGATTATGCACC
GGTAGTCCGGTTCGTCAATAAAATTCTGTTGGATGGCATCAGTACCGGTGCTTCCGATATTCATTTCGAGCACTACGAGA
AGACCTTTCGGGTCCGATATCGCCGGGATGGCATTTTACATGAGGTCGCCTCCCCACCTGTCAGCATCGCATCCCGATTG
ACAGCGCGCATCAAGGTGATGTCCAAGCTCAACGTTTCTGAGCGCCGAGTTCCCCAGGACGGACGCATCAAGATGGTATT
GTCCCGAAACCGCTCTATTGACTTCCGTGTGAATACCTGCCCTACACTCTATGGAGAAAAAATAGTGCTGCGCATCCTCG
ATTCCACCAGCACTCAACTGGGCATTGAGGCTTTGGGCTTGGAGCCTCAGCAAATGAATGCCTTTAATGCAGCAATTAAT
AAACCCTATGGAATGATTTTGGTCACGGGCCCAACCGGCAGCGGAAAAACAGTCTCGCTTTATACAGCGCTAAATATTCT
GAATAAACCTGAGATCAACATATCCACTGTCGAGGACCCGGTTGAAATACAGGTCCAGGGCATCAATCAGGTCAATACCA
ATACAAAAACAGGCTTGACCTTTGCGTCAGCCCTCCGGGCATTCCTTCGCCAGGACCCTGACATTATAATGGTGGGTGAA
ATACGCGACCTGGAAACAGCCGAGATTGCAGTCAAGGCCGCGCAAACCGGTCACTTGGTGCTATCCACCCTGCATACCAA
TGACGCGCCACAAACCCTCACGCGCCTGGCCAACATGGGTATACCTGCCTTCAGTATCGCATCATCCGTCCTATTGATAA
TGGCGCAACGTCTTACACGGCGCCTGTGCCAACACTGCAAAGAAGCCGAAGCCTTGCCCAAAGAAGCACTTCTTAAGGAG
GGCTTCAAAGAGAGTGAACTGAAGGATCTCACCACCTATAGAGCTGTAGGATGTGATCTCTGCGCTCAAGGATACAAGGG
TCGACTTGGTATATTTCAGGTTATGCCCATCTCAAGTAATATGGAAAAGCTGATCATGGAGGGCGGTAACTCCATGCAAC
TTGAGGCATTGGGAAAATCTGAAGGGACTCTAGATCTGCGCACATCTGGCCTCAATAAAGTAAGAGCAGGCCTTACCAGC
CTAGAAGAGATCAACCGGGTAACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.19

100

0.56
  pilB Acinetobacter baumannii D1279779

52.007

100

0.523
  pilB Acinetobacter baylyi ADP1

51.045

100

0.514
  pilB Vibrio cholerae strain A1552

50.476

92.105

0.465
  pilF Neisseria gonorrhoeae MS11

46.892

98.772

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

47.348

92.632

0.439
  pilB Vibrio campbellii strain DS40M4

47.148

92.281

0.435
  pilF Thermus thermophilus HB27

37.255

98.421

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

37.705

96.316

0.363