Detailed information NTG0700710

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MN084_RS12485
• Genome accession: NZ_CP099567
• Coordinates: 2262218..2263090 (+)
• Length: 290 a.a.
• NCBI ID: WP_241086174.1
• Uniprot ID: -
• Organism: Candidatus Vondammii sp. HM_W22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2257218..2268090

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MN084_RS12455	-	2257324..2257515 (-)	192	WP_241086179.1	hypothetical protein	-
MN084_RS12460	-	2257815..2257967 (-)	153	WP_241086178.1	hypothetical protein	-
MN084_RS12465	-	2258066..2258545 (-)	480	WP_241086177.1	pilin	-
MN084_RS12470	-	2258765..2258895 (-)	131	Protein_2455	transposase	-
MN084_RS12475	pilB	2259174..2260886 (+)	1713	WP_241086176.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MN084_RS12480	pilC	2260892..2262127 (+)	1236	WP_241086175.1	type II secretion system F family protein	Machinery gene
MN084_RS12485	pilD	2262218..2263090 (+)	873	WP_241086174.1	A24 family peptidase	Machinery gene
MN084_RS12490	coaE	2263096..2263695 (+)	600	WP_241086173.1	dephospho-CoA kinase	-
MN084_RS12495	zapD	2264179..2264475 (+)	297	WP_330178081.1	cell division protein ZapD	-
MN084_RS12500	-	2264634..2264834 (+)	201	WP_241086172.1	DNA gyrase inhibitor YacG	-
MN084_RS12505	-	2264902..2265843 (-)	942	WP_241086171.1	Nudix family hydrolase	-
MN084_RS12510	argJ	2265853..2267061 (-)	1209	WP_241086170.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31941.69 Da
• Isoelectric Point: 6.8236

>NTDB_id=700710 MN084_RS12485 WP_241086174.1 2262218..2263090(+) (pilD) [Candidatus Vondammii sp. HM_W22]
MSILQLLHSNPALFLISITLLSLTIGSFLNVVAYRLPLIIEREWKGQCRELLELSKEDGGDSISLSHPRSACPKCGHQIT
AWENIPVISYLILQGRCSECNRPISIRYPLVELTTALLSLVVAWHFGFSWQTAVAVPLTWALIALTLIDFDHQLLPDSIV
LPALWGGLLISLFGVFADTHSAIIGAITGYLSLWTIFHLFKLLTGKEGMGYGDFKLLALFGVWLGWQSLIQIMLLSSVVG
AVVGISLILFRGRDRNIPIPFGPYLAAAGWISMLWGDQINAAYLNWAGLG

Nucleotide

• Length: 873 bp

>NTDB_id=700710 MN084_RS12485 WP_241086174.1 2262218..2263090(+) (pilD) [Candidatus Vondammii sp. HM_W22]
ATGAGCATCCTGCAACTACTGCACTCAAACCCAGCCCTCTTCCTGATTTCCATCACCCTCCTCAGCCTCACCATTGGCAG
TTTCCTGAACGTCGTCGCCTACCGTCTGCCCCTGATCATCGAGCGCGAATGGAAAGGTCAATGCAGGGAGCTACTGGAAC
TTTCTAAAGAAGATGGTGGCGACAGCATCAGTCTGAGCCATCCTCGCTCAGCCTGTCCAAAATGTGGCCATCAGATAACA
GCTTGGGAGAATATCCCTGTCATCAGCTATTTGATACTTCAAGGACGCTGCTCCGAGTGCAATAGGCCGATTTCAATTCG
CTATCCTCTGGTTGAATTGACAACCGCTCTGCTCTCATTGGTAGTGGCCTGGCACTTTGGGTTTAGCTGGCAAACCGCAG
TAGCGGTTCCTCTGACATGGGCGCTGATCGCTCTGACCCTGATCGATTTTGATCATCAGTTACTGCCTGACTCTATTGTC
CTCCCAGCTCTTTGGGGTGGGCTGCTGATCAGTCTATTCGGTGTTTTTGCCGATACTCACTCCGCTATTATCGGAGCTAT
TACAGGCTATCTCTCACTCTGGACCATCTTTCATCTATTCAAACTGCTGACAGGTAAAGAGGGCATGGGCTATGGTGACT
TCAAGCTGCTCGCTCTCTTCGGCGTCTGGCTTGGCTGGCAGTCGCTTATCCAGATTATGCTGCTCTCATCGGTGGTTGGT
GCAGTGGTTGGTATCTCGCTTATCCTGTTCAGGGGCAGAGACCGCAACATCCCTATACCGTTTGGCCCCTACCTTGCCGC
CGCAGGCTGGATCAGCATGCTCTGGGGTGACCAGATCAATGCCGCCTATCTGAATTGGGCCGGCCTGGGATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (138-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.389	99.31	0.51
pilD	Vibrio campbellii strain DS40M4	51.228	98.276	0.503
pilD	Acinetobacter baumannii D1279779	49.648	97.931	0.486
pilD	Acinetobacter nosocomialis M2	49.648	97.931	0.486
pilD	Neisseria gonorrhoeae MS11	47.015	92.414	0.434