Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PCP45_RS19210 Genome accession   NZ_CP115270
Coordinates   4027299..4027763 (+) Length   154 a.a.
NCBI ID   WP_124089607.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4022299..4032763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP45_RS19185 (PCP45_19190) yacG 4022469..4022669 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP45_RS19190 (PCP45_19195) coaE 4022666..4023277 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP45_RS19195 (PCP45_19200) pilD 4023274..4024146 (-) 873 WP_134279316.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP45_RS19200 (PCP45_19205) pilC 4024147..4025364 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PCP45_RS19205 (PCP45_19210) pilB 4025368..4027068 (-) 1701 WP_128672463.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP45_RS19210 (PCP45_19215) pilA 4027299..4027763 (+) 465 WP_124089607.1 pilin Machinery gene
  PCP45_RS19215 (PCP45_19220) - 4027829..4029214 (+) 1386 WP_074222679.1 O-antigen ligase family protein -
  PCP45_RS19225 (PCP45_19230) nadC 4029395..4030243 (-) 849 WP_023098057.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16253.62 Da        Isoelectric Point: 9.0066

>NTDB_id=700688 PCP45_RS19210 WP_124089607.1 4027299..4027763(+) (pilA) [Pseudomonas aeruginosa strain F019]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSTTPNNTQYDIGFTE
STLLDGSGKSQIKVTDNKDGTVEMVATLGKSSGSAIKGAVITVSRDAQGVWNCKISKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=700688 PCP45_RS19210 WP_124089607.1 4027299..4027763(+) (pilA) [Pseudomonas aeruginosa strain F019]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCACGACTCCTAACAATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCAAGGTAACGGACAATAAAGATGGCACCGTTGAGATGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAACTGCA
AAATCTCCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  pilA Pseudomonas aeruginosa PAK

42.308

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.83

99.351

0.416
  pilA Vibrio parahaemolyticus RIMD 2210633

38.961

100

0.39
  pilA Vibrio cholerae strain A1552

36.364

100

0.364
  pilA Vibrio cholerae C6706

36.364

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.364