Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGM19_RS16005 Genome accession   NZ_CP099548
Coordinates   3357302..3358477 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain AJ6079     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3352302..3363477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM19_RS15980 (NGM19_15980) - 3352965..3353438 (-) 474 WP_129261207.1 hypothetical protein -
  NGM19_RS15985 (NGM19_15985) guaD 3353624..3354949 (+) 1326 WP_252665731.1 guanine deaminase -
  NGM19_RS15990 (NGM19_15990) hpt 3355166..3355693 (+) 528 WP_252665733.1 hypoxanthine phosphoribosyltransferase -
  NGM19_RS15995 (NGM19_15995) - 3355923..3356174 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  NGM19_RS16000 (NGM19_16000) - 3356224..3356922 (-) 699 WP_039047176.1 DsbC family protein -
  NGM19_RS16005 (NGM19_16005) pilU 3357302..3358477 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGM19_RS16010 (NGM19_16010) mnmE 3358533..3359888 (-) 1356 WP_212639625.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  NGM19_RS16015 (NGM19_16015) yidC 3359979..3361736 (-) 1758 WP_151825921.1 membrane protein insertase YidC -
  NGM19_RS16020 (NGM19_16020) yidD 3361742..3362062 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  NGM19_RS16025 (NGM19_16025) rnpA 3362072..3362467 (-) 396 WP_004950005.1 ribonuclease P protein component -
  NGM19_RS16030 (NGM19_16030) rpmH 3362496..3362630 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=700626 NGM19_RS16005 WP_004914541.1 3357302..3358477(+) (pilU) [Acinetobacter junii strain AJ6079]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=700626 NGM19_RS16005 WP_004914541.1 3357302..3358477(+) (pilU) [Acinetobacter junii strain AJ6079]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CGGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCAAGGTTTCGGGTCAATGTTTTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTTTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTACGAACTCAAGATGGTCAAGGACGTCGTGCCGCGATTGAAA
TCCTCCTCAATACGCCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGTGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTGTATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAATTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGAATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366