Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NGM67_RS18980 Genome accession   NZ_CP099545
Coordinates   3006278..3008005 (-) Length   575 a.a.
NCBI ID   WP_420875510.1    Uniprot ID   -
Organism   Photobacterium damselae strain XP-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3001278..3013005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM67_RS18955 (NGM67_18955) - 3001667..3001864 (+) 198 WP_036764707.1 DUF2970 domain-containing protein -
  NGM67_RS18960 (NGM67_18960) dam 3001848..3002690 (-) 843 WP_065172417.1 adenine-specific DNA-methyltransferase -
  NGM67_RS18965 (NGM67_18965) - 3002798..3004306 (-) 1509 WP_106141978.1 SPOR domain-containing protein -
  NGM67_RS18970 (NGM67_18970) aroB 3004321..3005400 (-) 1080 WP_252690006.1 3-dehydroquinate synthase -
  NGM67_RS18975 (NGM67_18975) aroK 3005430..3005948 (-) 519 WP_036765246.1 shikimate kinase AroK -
  NGM67_RS18980 (NGM67_18980) pilQ 3006278..3008005 (-) 1728 WP_420875510.1 type IV pilus secretin PilQ Machinery gene
  NGM67_RS18985 (NGM67_18985) - 3008029..3008544 (-) 516 WP_036764704.1 pilus assembly protein PilP -
  NGM67_RS18990 (NGM67_18990) - 3008553..3009143 (-) 591 WP_068945317.1 type 4a pilus biogenesis protein PilO -
  NGM67_RS18995 (NGM67_18995) - 3009140..3009781 (-) 642 WP_068945318.1 PilN domain-containing protein -
  NGM67_RS19000 (NGM67_19000) pilM 3009778..3010776 (-) 999 WP_068945319.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62768.22 Da        Isoelectric Point: 4.8490

>NTDB_id=700581 NGM67_RS18980 WP_420875510.1 3006278..3008005(-) (pilQ) [Photobacterium damselae strain XP-11]
MKRMLSVSLGWQRCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTIQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKASSTDKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEDIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=700581 NGM67_RS18980 WP_420875510.1 3006278..3008005(-) (pilQ) [Photobacterium damselae strain XP-11]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGTTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAACCGTGGACGAG
GCGTGCTCACTATTCAGTTAGCCAAACCTCAATCTTTGGTTGATATGCGCCGTTCAGGGAAGCAATTACACATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTACTCGATGTGGTCGACTTTGCAACGGTTGTCCGTTCGATTGAAAC
ATTTCGCACCGAGAAAGGTAGCAAGCTTATTCTCAATATGGCTGGCGTATTTGATTATGATTACCAATTAAAAGGCTCTC
GTCTTGAAGTTGTTTTGAACGCCCAGAAAAAGAAAGCCAGCAGTACAGACAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCCATCTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTATTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACTGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGGTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCCAAACGTAAAGAAGATGAGTTAGCTTCGCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATCGCAGACCTTTTACTCGGGACTAAAGATGGAGTCAGTATGCTATCTCAACGCGGGTCTTTGCATG
TTGATGAGCGTACTAATGCCATAATCATTAAAGATATTCCTGAGAGTATTGATAGCATTGAAGATATTATCACGGCACTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAAGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGGGGCTCAATTGAAGGTAACTTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGTGCAATTACTCCTGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTGGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCGAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTAACCACCAATAAAAAAGCGGCTTACATCGAACAAGGTACAGAGATCCCTTATTTAGAAGCGGCATCTA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGTTTGATGGTCACACCACAAATTACCCCTGATGATAAATTG
GTGCTCGATCTGCTGGTAACGCAAGATAAAGTAGGGGAAACAGTTGTTACCGGAACCGGAGAAGCGGTCGCTATCGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAATGGCGAGACTGTAGTCTTAGGGGGTATCTATCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGCGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCCAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II and III secretion system protein (Secretin)

(415-573)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

60.946

99.304

0.605
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.946

99.304

0.605
  pilQ Vibrio campbellii strain DS40M4

60.394

97.043

0.586
  pilQ Legionella pneumophila strain Lp02

42.831

94.609

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.831

94.609

0.405
  pilQ Pseudomonas aeruginosa PAK

41.281

97.739

0.403
  comQ Acinetobacter baylyi ADP1

40.564

98.609

0.4
  pilQ Acinetobacter baumannii D1279779

38.966

100

0.393
  pilQ Acinetobacter baumannii strain A118

38.966

100

0.393