Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NG829_RS05680 Genome accession   NZ_CP099532
Coordinates   1327827..1329560 (+) Length   577 a.a.
NCBI ID   WP_267122126.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain LT6-16-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1322827..1334560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG829_RS05655 (NG829_05650) coaE 1323889..1324518 (-) 630 WP_267122124.1 dephospho-CoA kinase -
  NG829_RS05660 (NG829_05655) - 1324532..1325395 (-) 864 WP_017910397.1 A24 family peptidase -
  NG829_RS05665 (NG829_05660) pilC 1325402..1326661 (-) 1260 WP_267122125.1 type II secretion system F family protein Machinery gene
  NG829_RS05675 (NG829_05670) - 1327030..1327473 (+) 444 WP_318526824.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NG829_RS05680 (NG829_05675) pilB 1327827..1329560 (+) 1734 WP_267122126.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NG829_RS05685 (NG829_05680) pilR 1329791..1331179 (-) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator
  NG829_RS05690 (NG829_05685) - 1331262..1332875 (-) 1614 WP_267122127.1 ATP-binding protein -
  NG829_RS05695 (NG829_05690) sucC 1333267..1334436 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62584.87 Da        Isoelectric Point: 5.6425

>NTDB_id=700434 NG829_RS05680 WP_267122126.1 1327827..1329560(+) (pilB) [Xanthomonas sacchari strain LT6-16-1]
MSAVITANLVGITGIARRLVQDGALDEAAARAAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGSGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=700434 NG829_RS05680 WP_267122126.1 1327827..1329560(+) (pilB) [Xanthomonas sacchari strain LT6-16-1]
ATGAGCGCTGTGATCACCGCCAACCTCGTCGGCATCACCGGGATCGCACGCCGCCTTGTGCAGGACGGCGCGCTGGACGA
AGCCGCGGCGCGGGCCGCGATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCCGCGCAGCTGGCCGCCGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTCTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCAGCGGTGGCGACACCGGCATCGATGC
CAAGGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGTCGCGGCGCGTCCGACATCC
ACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGGTCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGAACAAGCAGATCGATTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGTTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTCGGCTACGAGCCCGAGCAGCAGAAGCTG
TTCCTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACTGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTGACCTTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGTCCGGTCCAATTGCCGGAG
AAGGCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAAGCGGTCGGCTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGC
AGGGCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCGGCCTTGCTCAAG
GCCCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.844

100

0.549
  pilB Acinetobacter baumannii D1279779

54.389

100

0.548
  pilB Legionella pneumophila strain ERS1305867

51.142

98.614

0.504
  pilB Vibrio cholerae strain A1552

50

99.48

0.497
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.721

100

0.388
  pilF Thermus thermophilus HB27

40.27

89.948

0.362