Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5E07_RS14615 Genome accession   NZ_CP098732
Coordinates   3038832..3040538 (+) Length   568 a.a.
NCBI ID   WP_116758773.1    Uniprot ID   -
Organism   Acinetobacter tibetensis strain Y-23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033832..3045538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E07_RS14575 (M5E07_14575) nusA 3034585..3036069 (-) 1485 WP_116758769.1 transcription termination factor NusA -
  M5E07_RS14580 (M5E07_14580) rimP 3036105..3036629 (-) 525 WP_252220325.1 ribosome maturation factor RimP -
  M5E07_RS14605 (M5E07_14605) secG 3037417..3037746 (-) 330 WP_116758771.1 preprotein translocase subunit SecG -
  M5E07_RS14610 (M5E07_14610) tpiA 3037760..3038551 (-) 792 WP_252220328.1 triose-phosphate isomerase -
  M5E07_RS14615 (M5E07_14615) pilB 3038832..3040538 (+) 1707 WP_116758773.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M5E07_RS14620 (M5E07_14620) pilC 3040567..3041793 (+) 1227 WP_252220331.1 type II secretion system F family protein Machinery gene
  M5E07_RS14625 (M5E07_14625) pilD 3041793..3042653 (+) 861 WP_252220334.1 A24 family peptidase Machinery gene
  M5E07_RS14630 (M5E07_14630) coaE 3042656..3043273 (+) 618 WP_252220337.1 dephospho-CoA kinase -
  M5E07_RS14635 (M5E07_14635) - 3043270..3044187 (-) 918 WP_116758777.1 DMT family transporter -
  M5E07_RS14640 (M5E07_14640) rlmB 3044222..3044974 (-) 753 WP_116758778.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  M5E07_RS14645 (M5E07_14645) - 3045111..3045434 (-) 324 WP_016168405.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63560.02 Da        Isoelectric Point: 5.2500

>NTDB_id=697483 M5E07_RS14615 WP_116758773.1 3038832..3040538(+) (pilB) [Acinetobacter tibetensis strain Y-23]
MTALQGSPRFTGFIRRLVEDGIVSTENMQTAVSSAKKANQDIVPYLIENLKLKPIVIAEKISEEFGEPLFDLSTYDSAHI
LRDVVEDKLITKFRVLPIFRRGHQLFVATSNPTHIDAMDAMRFNSKLSIEAIIVEHDKLEKIIEQNYAAESTFEFDDDFE
LHVHEEHTDQEDNDDQPQEDEAPIVKYINKLLIDAIRMGASDLHFEPYEKVYRVRYRVDGVLRQIATPPLQLANRLASRL
KVMSQMDIAEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFMEALEKPQG
MLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPTEVPKNSLLEMGFSE
QEIQQPDFKIFQPVGCSECRDGYKGRVGIYEVMKITSEISKIIMEDGNAIQIAEASAKLGFNNLRLSGLKKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=697483 M5E07_RS14615 WP_116758773.1 3038832..3040538(+) (pilB) [Acinetobacter tibetensis strain Y-23]
ATGACAGCACTACAAGGTTCTCCACGATTCACAGGTTTTATTCGCCGTTTGGTCGAAGATGGAATCGTTTCTACTGAAAA
CATGCAGACTGCTGTTAGCAGTGCAAAGAAAGCAAACCAAGATATTGTCCCTTATTTAATTGAGAATTTAAAACTCAAAC
CCATTGTCATTGCCGAAAAAATTTCTGAAGAGTTTGGTGAGCCGTTATTTGATCTTTCGACTTATGACTCTGCTCATATT
TTAAGAGATGTCGTTGAAGACAAACTGATTACCAAATTCCGCGTTTTGCCCATTTTTCGACGTGGGCATCAACTCTTTGT
AGCCACCAGCAATCCGACGCATATAGATGCTATGGATGCCATGCGTTTCAACAGTAAGCTCAGCATTGAAGCAATTATTG
TTGAACATGACAAATTAGAAAAAATTATTGAACAAAATTATGCCGCAGAAAGTACCTTTGAGTTTGATGATGACTTTGAA
TTGCATGTCCATGAAGAGCATACAGATCAAGAAGACAACGATGATCAACCTCAAGAAGATGAGGCCCCGATTGTTAAATA
TATCAATAAATTATTGATTGATGCGATACGCATGGGTGCATCGGACTTACATTTTGAACCGTATGAAAAAGTGTATCGTG
TACGCTACCGTGTAGATGGTGTTTTACGCCAGATTGCTACGCCTCCATTACAGTTAGCCAACCGTCTTGCTTCACGTTTA
AAGGTTATGTCTCAAATGGACATTGCTGAAAAACGTGTGCCTCAAGATGGACGGATTAAACTTAAAATATCAAAAACGAA
AGCCATCGATTTTCGTGTCAACTCACTGCCGACACTTTTTGGTGAAAAAATTGTGTTGCGTATTTTAGATCCTTCGAGTG
CAATGCTGGGTATTGATGCCTTAGGTTATGAACCTGAACAAAAAGCTTTATTTATGGAAGCTCTGGAAAAACCACAAGGG
ATGCTGCTCATCACAGGTCCCACAGGTTCAGGTAAAACGGTTTCTCTCTATACTGGTCTGAATATTCTCAACAAAGAAGA
TACCAATATTTCAACCGCTGAAGATCCTGTCGAAATTAACTTGGAAGGTATTAATCAGGTTAACGTGAACAATAAAGTTG
GTCTAACCTTCTCAGCCGCATTAAAGTCTTTCTTACGACAAGATCCAGACATTGTCATGGTCGGTGAGATCCGAGACTTA
GAAACTGCTGAAATTGCGATTAAAGCCGCGCAAACGGGGCATATGGTGATGTCTACCCTACATACCAATAGCGCACCAGA
AACCCTGACTCGTTTAAGAAATATGGGGGTCCCTTCCTTTAATATTGCAACCTCTGTAAACTTAGTGATTGCACAACGAC
TTGCTCGACGACTCTGTCCACAGTGTAAAGTCCCTACTGAAGTACCCAAAAATAGTTTGTTAGAAATGGGCTTTAGCGAA
CAAGAGATACAACAGCCAGATTTCAAAATTTTTCAACCTGTCGGTTGTTCAGAATGTCGAGATGGCTATAAAGGGCGTGT
GGGGATCTATGAAGTAATGAAAATTACATCTGAAATATCCAAGATTATTATGGAAGATGGTAATGCAATTCAAATTGCAG
AAGCATCGGCAAAATTGGGGTTTAATAATCTACGTCTTTCAGGTTTAAAGAAGGTCATGCAAGGGGTAACTTCACTACAA
GAAGTCAATCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.123

100

0.794
  pilB Acinetobacter baylyi ADP1

76.316

100

0.766
  pilB Legionella pneumophila strain ERS1305867

51.97

93.838

0.488
  pilF Neisseria gonorrhoeae MS11

49.464

98.592

0.488
  pilB Vibrio cholerae strain A1552

48.404

99.296

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

52.224

91.021

0.475
  pilB Vibrio campbellii strain DS40M4

47.935

98.063

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

33.819

100

0.368
  pilF Thermus thermophilus HB27

36.991

99.472

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.365

97.535

0.364