Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NDN13_RS16240 Genome accession   NZ_CP098480
Coordinates   3400983..3402695 (-) Length   570 a.a.
NCBI ID   WP_251116199.1    Uniprot ID   -
Organism   Acinetobacter sp. C32I     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3395983..3407695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN13_RS16210 (NDN13_16205) - 3396056..3396382 (+) 327 WP_004876475.1 pyrimidine/purine nucleoside phosphorylase -
  NDN13_RS16215 (NDN13_16210) rlmB 3396575..3397321 (+) 747 WP_065343633.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NDN13_RS16220 (NDN13_16215) - 3397351..3398262 (+) 912 WP_251116196.1 DMT family transporter -
  NDN13_RS16225 (NDN13_16220) coaE 3398259..3398864 (-) 606 WP_251116197.1 dephospho-CoA kinase -
  NDN13_RS16230 (NDN13_16225) pilD 3398866..3399726 (-) 861 WP_251116198.1 A24 family peptidase Machinery gene
  NDN13_RS16235 (NDN13_16230) pilC 3399732..3400958 (-) 1227 WP_004656216.1 type II secretion system F family protein Machinery gene
  NDN13_RS16240 (NDN13_16235) pilB 3400983..3402695 (-) 1713 WP_251116199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NDN13_RS16245 (NDN13_16240) tpiA 3403068..3403859 (+) 792 WP_251116200.1 triose-phosphate isomerase -
  NDN13_RS16250 (NDN13_16245) secG 3403872..3404201 (+) 330 WP_251116201.1 preprotein translocase subunit SecG -
  NDN13_RS16270 (NDN13_16265) rimP 3404829..3405353 (+) 525 WP_251116202.1 ribosome maturation factor RimP -
  NDN13_RS16275 (NDN13_16270) nusA 3405391..3406875 (+) 1485 WP_004656210.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63355.58 Da        Isoelectric Point: 4.9084

>NTDB_id=696174 NDN13_RS16240 WP_251116199.1 3400983..3402695(-) (pilB) [Acinetobacter sp. C32I]
MSGLHTSPKFSGFIRRLVEDGHMSTATMQTAIDTAKKSQQDIVAHLIEQHRLSPLTIAETIAVEFGEPLFDLAVYDTALL
PRELADNKLIQKHKVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGESED
FDLDIDVSQTPNQEEEDTPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKEEINIPEQSLLELGF
TEQDLNDPEFKIFQPVGCAECREGYKGRVGIYEVMKVTPEISRLIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=696174 NDN13_RS16240 WP_251116199.1 3400983..3402695(-) (pilB) [Acinetobacter sp. C32I]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGATGGACACATGTCTACGGCAAC
GATGCAGACCGCAATTGATACGGCTAAAAAATCACAACAAGATATCGTTGCACATTTGATTGAACAGCATCGCTTATCTC
CTCTGACCATTGCGGAAACCATTGCCGTCGAATTCGGTGAGCCGCTTTTCGATCTGGCAGTATACGATACAGCTTTACTA
CCTCGCGAACTGGCGGATAACAAGCTCATTCAAAAGCATAAAGTTGCTCCGCTACTACAACGTGGGCAAATTTTATATGT
GGCGACCAGTAACCCCACCAATATCGAAGCTTTGGATGCAATTCGCTTCAATACCAAACTGAATATTGAACCGATTATAG
TTGAACACACTAAACTCGAAAAACTGATTGAACAGCAGTTTGGCGATTCTGGTAGTTTTGATTTTGGTGAAAGTGAAGAC
TTTGATCTCGACATTGATGTCAGTCAAACCCCAAACCAAGAGGAAGAAGACACTCCACAAGGCGACGAATCTCCAATTGT
TAAATATATTAACAAACTGCTCATTGATGCGATTCGTATGGGTGCCTCTGACTTACATTTTGAACCTTATGAAAAAATGT
ACCGTGTCCGCTATCGTGTTGATGGTGTACTCCGTCAGATTGCCAATCCGCCCTTACAAATGGCCACTCGTCTCGCCTCG
CGTTTAAAAGTCATGTCACAAATGGACATTTCTGAAAAACGGATGCCGCAAGATGGACGTATTAAACTTAAACTTTCCAA
GACCAAAGCCATCGACTTTCGTGTCAACTCACTCCCCACCCTATTTGGCGAAAAGCTGGTGCTACGTATTCTCGATCCAT
CGAGTGCTATGTTGGGGATCGAAGCACTCGGTTATGAAGATGAGCAAAAAGCCTTATTTATGGATGCACTTGAAAAACCT
CAAGGCATGCTACTGATTACGGGTCCAACAGGTTCAGGTAAAACCGTCTCTTTGTATACAGGTTTAAATATCCTCAATAC
TGAAGACACCAATATTTCAACTGCCGAAGATCCAGTCGAAATTAACTTAGAAGGGATTAATCAGGTTAACGTCAATCCAA
AAACAGGACTCACCTTCTCTGCAGCCCTGAAAGCGTTTTTACGCCAAGATCCTGATGTGATCATGGTCGGGGAGATCCGT
GATCTGGAAACCGCTGAAATTGCCATTAAAGCCGCACAAACGGGCCATATGGTGATGTCAACCCTACACACCAACAGTGC
ACCAGAAACCTTGACCCGTTTACGTAACATGGGGGTTCCTTCGTTCAACATCGCAACCTCAGTCAATCTGGTGATTGCAC
AACGCTTGGCACGTCGTCTCTGTGGCCAATGTAAAGAAGAAATTAATATCCCTGAACAAAGTCTGTTGGAACTCGGCTTT
ACTGAACAAGATTTAAATGATCCTGAATTCAAGATTTTTCAACCTGTTGGTTGTGCTGAATGTCGTGAAGGCTATAAAGG
TCGTGTGGGGATTTACGAAGTCATGAAGGTTACACCAGAAATTTCCAGACTGATTATGGAAGACGGCAATGCCTTACAGA
TTGCAGCAGCATCAGAACAAGCAGGATTTAATAATCTACGTCGTTCAGGTTTGAAAAAAGTCATGCAGGGAATTACCTCT
TTGCAGGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.786

100

0.819
  pilB Acinetobacter baylyi ADP1

76.532

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.506

95.088

0.509
  pilF Neisseria gonorrhoeae MS11

49.215

100

0.495
  pilB Vibrio cholerae strain A1552

48.837

98.07

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

48.741

97.544

0.475
  pilB Vibrio campbellii strain DS40M4

51.252

91.053

0.467
  pilF Thermus thermophilus HB27

37.411

98.947

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.824

100

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.91

93.333

0.363