Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NDN12_RS16325 Genome accession   NZ_CP098479
Coordinates   3570539..3572251 (+) Length   570 a.a.
NCBI ID   WP_251110237.1    Uniprot ID   -
Organism   Acinetobacter sp. C26G     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3565539..3577251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN12_RS16290 (NDN12_16285) nusA 3566357..3567841 (-) 1485 WP_004802322.1 transcription termination factor NusA -
  NDN12_RS16295 (NDN12_16290) rimP 3567879..3568403 (-) 525 WP_005154900.1 ribosome maturation factor RimP -
  NDN12_RS16315 (NDN12_16310) secG 3569033..3569362 (-) 330 WP_167250335.1 preprotein translocase subunit SecG -
  NDN12_RS16320 (NDN12_16315) tpiA 3569375..3570166 (-) 792 WP_251110236.1 triose-phosphate isomerase -
  NDN12_RS16325 (NDN12_16320) pilB 3570539..3572251 (+) 1713 WP_251110237.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NDN12_RS16330 (NDN12_16325) pilC 3572276..3573505 (+) 1230 WP_251110238.1 type II secretion system F family protein Machinery gene
  NDN12_RS16335 (NDN12_16330) pilD 3573518..3574378 (+) 861 WP_251110239.1 A24 family peptidase Machinery gene
  NDN12_RS16340 (NDN12_16335) coaE 3574380..3574985 (+) 606 WP_251110240.1 dephospho-CoA kinase -
  NDN12_RS16345 (NDN12_16340) - 3574982..3575893 (-) 912 WP_251110241.1 DMT family transporter -
  NDN12_RS16350 (NDN12_16345) rlmB 3575923..3576669 (-) 747 WP_004656224.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NDN12_RS16355 (NDN12_16350) - 3576870..3577196 (-) 327 WP_004802302.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63256.54 Da        Isoelectric Point: 5.0134

>NTDB_id=696126 NDN12_RS16325 WP_251110237.1 3570539..3572251(+) (pilB) [Acinetobacter sp. C26G]
MSGLHTSPKFSGFIRRLVEDGHMSTTTMQTAIDAAKKAQQDIVAHLIEQHRLSPLTIAETIAAEFGEPLFDLAVYDTALL
PRDLADNKLIQKHKVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGDSEE
FDLDIDVSQDPTKEEDDTPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKEEISIPKQSLLELGF
TEQDLNDPEFKIFQPIGCAECREGYKGRVGIYEVMKVTPEISRLIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=696126 NDN12_RS16325 WP_251110237.1 3570539..3572251(+) (pilB) [Acinetobacter sp. C26G]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGATGGACACATGTCCACCACAAC
CATGCAAACCGCAATTGATGCGGCTAAAAAAGCACAGCAAGATATTGTTGCACACCTGATTGAACAACATCGTTTATCAC
CACTGACCATTGCTGAAACGATTGCTGCGGAGTTTGGCGAACCACTCTTCGATCTGGCTGTATATGACACGGCTTTGCTG
CCGCGTGATCTGGCAGACAACAAATTAATTCAAAAACATAAAGTTGCCCCATTACTACAGCGCGGGCAGATTTTATATGT
CGCGACTAGTAATCCAACCAATATTGAAGCGTTGGATGCGATTCGCTTCAACACCAAACTCAATATTGAACCAATTATTG
TTGAGCACACTAAACTCGAAAAACTGATTGAACAACAATTCGGTGATTCTGGCAGTTTCGATTTTGGCGACAGCGAAGAA
TTTGATCTAGACATTGATGTTAGCCAAGACCCTACGAAAGAAGAGGATGATACGCCACAAGGCGATGAGTCTCCGATTGT
AAAATATATTAATAAGTTATTGATTGATGCCATTCGTATGGGTGCATCTGACTTACATTTTGAGCCTTATGAAAAAATGT
ACCGTGTCCGTTATCGTGTCGATGGTGTACTCCGTCAGATTGCTAACCCACCATTACAAATGGCAACACGTCTCGCCTCG
CGTTTAAAAGTCATGTCGCAAATGGATATTTCCGAGAAACGTATGCCACAAGATGGGCGTATTAAACTCAAACTATCCAA
GACCAAAGCAATTGATTTCCGTGTTAACTCACTTCCTACACTATTTGGTGAGAAACTGGTACTTCGTATTCTAGATCCGT
CTAGTGCCATGCTTGGCATTGAAGCGCTCGGTTATGAAGATGAGCAAAAAGCCTTATTTATGGATGCGCTGGATAAGCCT
CAAGGCATGCTACTGATTACAGGTCCAACAGGTTCTGGTAAAACAGTTTCGTTGTATACGGGTTTAAATATACTGAATAC
CGAAGACACCAATATTTCTACCGCCGAAGATCCTGTTGAAATTAACTTGGAAGGGATTAATCAGGTCAATGTGAATCCCA
AAACTGGACTCACCTTCTCCGCTGCACTCAAAGCTTTCTTACGCCAAGATCCGGATGTAATCATGGTCGGGGAGATTCGT
GATCTGGAAACCGCTGAAATTGCCATTAAAGCCGCACAAACGGGTCATATGGTGATGTCAACCCTACACACCAACAGTGC
ACCAGAAACCTTGACCCGATTACGCAATATGGGGGTCCCTTCGTTCAACATCGCAACCTCAGTCAATCTGGTCATTGCGC
AACGCTTAGCGCGTCGCCTCTGTGGTCAATGCAAGGAAGAAATTAGTATTCCTAAACAAAGCCTGTTAGAACTCGGCTTT
ACTGAACAAGATTTAAATGACCCTGAATTCAAAATTTTTCAACCTATTGGTTGTGCTGAGTGCCGTGAAGGCTATAAAGG
TCGTGTGGGGATTTACGAGGTTATGAAGGTCACTCCAGAAATTTCCAGACTAATTATGGAAGATGGTAATGCCTTACAAA
TTGCGGCAGCATCAGAACAAGCAGGTTTTAATAATCTACGTCGTTCAGGTTTGAAAAAGGTCATGCAGGGAATTACCTCT
TTGCAAGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.867

100

0.832
  pilB Acinetobacter baylyi ADP1

76.182

100

0.763
  pilB Legionella pneumophila strain ERS1305867

53.659

93.509

0.502
  pilF Neisseria gonorrhoeae MS11

48.668

98.772

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.741

97.544

0.475
  pilB Vibrio cholerae strain A1552

49.094

96.842

0.475
  pilB Vibrio campbellii strain DS40M4

51.158

90.877

0.465
  pilF Thermus thermophilus HB27

37.857

98.246

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

34.202

100

0.368