Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NAF16_RS00685 Genome accession   NZ_CP098021
Coordinates   149962..151698 (+) Length   578 a.a.
NCBI ID   WP_233921186.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144962..156698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS00665 (NAF16_00665) pilM 147268..148272 (+) 1005 WP_323694917.1 type IV pilus assembly protein PilM Machinery gene
  NAF16_RS00670 (NAF16_00670) pilN 148262..148852 (+) 591 WP_061057155.1 PilN domain-containing protein Machinery gene
  NAF16_RS00675 (NAF16_00675) pilO 148845..149435 (+) 591 WP_202642994.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAF16_RS00680 (NAF16_00680) pilP 149425..149937 (+) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  NAF16_RS00685 (NAF16_00685) pilQ 149962..151698 (+) 1737 WP_233921186.1 type IV pilus secretin PilQ Machinery gene
  NAF16_RS00690 (NAF16_00690) aroK 151886..152404 (+) 519 WP_004728569.1 shikimate kinase AroK -
  NAF16_RS00695 (NAF16_00695) aroB 152432..153517 (+) 1086 WP_020329448.1 3-dehydroquinate synthase -
  NAF16_RS00700 (NAF16_00700) - 153563..155071 (+) 1509 WP_323694918.1 AAA family ATPase -
  NAF16_RS00705 (NAF16_00705) - 155166..155996 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62410.35 Da        Isoelectric Point: 4.5373

>NTDB_id=693538 NAF16_RS00685 WP_233921186.1 149962..151698(+) (pilQ) [Vibrio fluvialis strain V13]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=693538 NAF16_RS00685 WP_233921186.1 149962..151698(+) (pilQ) [Vibrio fluvialis strain V13]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTGGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCCGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCCTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTACGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGTGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAACGTTAACCTCGCAGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCAGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACGACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACCTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTGCTGGATTTGAGCGTTACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTTCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCTGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.693

100

0.818
  pilQ Vibrio cholerae strain A1552

81.693

100

0.818
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382