Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NAF16_RS00675 Genome accession   NZ_CP098021
Coordinates   148845..149435 (+) Length   196 a.a.
NCBI ID   WP_202642994.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143845..154435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS00660 (NAF16_00660) - 144645..147125 (-) 2481 WP_222753359.1 penicillin-binding protein 1A -
  NAF16_RS00665 (NAF16_00665) pilM 147268..148272 (+) 1005 WP_323694917.1 type IV pilus assembly protein PilM Machinery gene
  NAF16_RS00670 (NAF16_00670) pilN 148262..148852 (+) 591 WP_061057155.1 PilN domain-containing protein Machinery gene
  NAF16_RS00675 (NAF16_00675) pilO 148845..149435 (+) 591 WP_202642994.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAF16_RS00680 (NAF16_00680) pilP 149425..149937 (+) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  NAF16_RS00685 (NAF16_00685) pilQ 149962..151698 (+) 1737 WP_233921186.1 type IV pilus secretin PilQ Machinery gene
  NAF16_RS00690 (NAF16_00690) aroK 151886..152404 (+) 519 WP_004728569.1 shikimate kinase AroK -
  NAF16_RS00695 (NAF16_00695) aroB 152432..153517 (+) 1086 WP_020329448.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22778.17 Da        Isoelectric Point: 4.7023

>NTDB_id=693536 NAF16_RS00675 WP_202642994.1 148845..149435(+) (pilO) [Vibrio fluvialis strain V13]
MANLQELELDEIVEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGERQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=693536 NAF16_RS00675 WP_202642994.1 148845..149435(+) (pilO) [Vibrio fluvialis strain V13]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGTTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCGCTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAGGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GTTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAGGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

80.102

100

0.801
  pilO Vibrio campbellii strain DS40M4

67.895

96.939

0.658