Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NAF16_RS00665 Genome accession   NZ_CP098021
Coordinates   147268..148272 (+) Length   334 a.a.
NCBI ID   WP_323694917.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142268..153272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS00645 (NAF16_00645) - 142418..142681 (-) 264 WP_020429299.1 DUF3624 domain-containing protein -
  NAF16_RS00650 (NAF16_00650) - 142810..143544 (-) 735 WP_061057153.1 glutathione peroxidase -
  NAF16_RS00655 (NAF16_00655) oxyR 143688..144584 (+) 897 WP_202641045.1 DNA-binding transcriptional regulator OxyR -
  NAF16_RS00660 (NAF16_00660) - 144645..147125 (-) 2481 WP_222753359.1 penicillin-binding protein 1A -
  NAF16_RS00665 (NAF16_00665) pilM 147268..148272 (+) 1005 WP_323694917.1 type IV pilus assembly protein PilM Machinery gene
  NAF16_RS00670 (NAF16_00670) pilN 148262..148852 (+) 591 WP_061057155.1 PilN domain-containing protein Machinery gene
  NAF16_RS00675 (NAF16_00675) pilO 148845..149435 (+) 591 WP_202642994.1 type 4a pilus biogenesis protein PilO Machinery gene
  NAF16_RS00680 (NAF16_00680) pilP 149425..149937 (+) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  NAF16_RS00685 (NAF16_00685) pilQ 149962..151698 (+) 1737 WP_233921186.1 type IV pilus secretin PilQ Machinery gene
  NAF16_RS00690 (NAF16_00690) aroK 151886..152404 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36874.19 Da        Isoelectric Point: 6.6959

>NTDB_id=693534 NAF16_RS00665 WP_323694917.1 147268..148272(+) (pilM) [Vibrio fluvialis strain V13]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRRSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADNAPFYKDIPLGSRQLVAATSGGNADLTSPQAHFIQD
LVERLARQIQLFLSLQGHQPLAGIWLSGGGAATEGLAQAISERLALPCELFNPLAEFTCRASLRRQPQVDLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=693534 NAF16_RS00665 WP_323694917.1 147268..148272(+) (pilM) [Vibrio fluvialis strain V13]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAATTGGATGCCAGCGAACAAGAGTTCGCGGTCTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTCACCGGAGAAAAAACTCTAGGGCGCAGAAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTCGATGTCGGGTTCAGCCAAACCTCATTGTGCATCGACTTTGCCGACAACGCACCGTTTTACAAAGACATTCCGT
TGGGATCCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAT
TTGGTTGAGCGTCTGGCGCGCCAGATCCAACTATTTCTGTCTCTGCAAGGCCATCAACCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGCGCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCGCCAGCCTCAGGTTGATTTGCAGCGTTTCAGTACTGCGGCAGGCTTG
GCGCTGCGCGGGCTCAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.497

100

0.653
  pilM Vibrio campbellii strain DS40M4

48.406

100

0.5