Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M8C01_RS14535 Genome accession   NZ_CP097858
Coordinates   3177579..3177992 (+) Length   137 a.a.
NCBI ID   WP_039987783.1    Uniprot ID   -
Organism   Vibrio owensii strain GL-605     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172579..3182992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8C01_RS14520 pdhR 3174611..3175378 (-) 768 WP_005438026.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  M8C01_RS14525 ampD 3175783..3176334 (-) 552 WP_264403756.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8C01_RS14530 nadC 3176427..3177314 (+) 888 WP_264403758.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M8C01_RS14535 pilA 3177579..3177992 (+) 414 WP_039987783.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  M8C01_RS14540 pilB 3177993..3179678 (+) 1686 WP_264403759.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M8C01_RS14545 pilC 3179712..3180935 (+) 1224 WP_264403760.1 type II secretion system F family protein Machinery gene
  M8C01_RS14550 pilD 3181008..3181877 (+) 870 WP_041058619.1 A24 family peptidase Machinery gene
  M8C01_RS14555 coaE 3181878..3182492 (+) 615 WP_264403761.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13859.75 Da        Isoelectric Point: 8.4778

>NTDB_id=693111 M8C01_RS14535 WP_039987783.1 3177579..3177992(+) (pilA) [Vibrio owensii strain GL-605]
MKTNKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVSKSELASGFATIKSVITPAELYIQENGAISGASPSDLGVSA
GANSLGTLSISGNAVVFTHNNGAVSGAAFTYSRGTSSGWTCALTGQPTGVDAPKGCS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=693111 M8C01_RS14535 WP_039987783.1 3177579..3177992(+) (pilA) [Vibrio owensii strain GL-605]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTAATGATTGTAGTGGCGATTATTGGTGTACT
GTCCGCAATTGCAGTCCCAGCATATAAAGATTATGTATCAAAGAGTGAGTTAGCATCTGGTTTTGCTACCATAAAATCAG
TAATCACTCCTGCCGAATTATATATACAAGAAAACGGCGCTATATCTGGAGCAAGCCCTAGTGACCTAGGAGTATCTGCT
GGTGCCAACAGCCTAGGAACACTATCTATCAGCGGCAATGCCGTTGTATTTACTCACAACAATGGGGCGGTATCTGGTGC
GGCTTTCACATATAGCAGAGGCACTAGTTCCGGCTGGACATGTGCTTTGACAGGACAACCAACTGGGGTTGATGCACCAA
AAGGTTGCTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50.704

100

0.526
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.474

100

0.438
  pilA Vibrio cholerae strain A1552

39.474

100

0.438
  pilA Vibrio cholerae C6706

39.474

100

0.438
  pilA Acinetobacter baumannii strain A118

40.69

100

0.431
  pilA Pseudomonas aeruginosa PAK

38

100

0.416