Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M8993_RS05735 Genome accession   NZ_CP097605
Coordinates   1254344..1254778 (-) Length   144 a.a.
NCBI ID   WP_250191216.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10185     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1249344..1259778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8993_RS05705 (M8993_05705) - 1249907..1250176 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M8993_RS05710 (M8993_05710) yacG 1250176..1250379 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M8993_RS05715 (M8993_05715) coaE 1250390..1251010 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  M8993_RS05720 (M8993_05720) - 1251025..1251747 (-) 723 WP_064968698.1 A24 family peptidase -
  M8993_RS05725 (M8993_05725) - 1251747..1252959 (-) 1213 Protein_1096 type II secretion system F family protein -
  M8993_RS05730 (M8993_05730) pilB 1252959..1254344 (-) 1386 WP_169029463.1 GspE/PulE family protein Machinery gene
  M8993_RS05735 (M8993_05735) pilA 1254344..1254778 (-) 435 WP_250191216.1 prepilin peptidase-dependent pilin Machinery gene
  M8993_RS05740 (M8993_05740) ampD 1254938..1255504 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8993_RS05745 (M8993_05745) rppH 1256090..1256686 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M8993_RS05750 (M8993_05750) - 1256689..1257483 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M8993_RS05755 (M8993_05755) lgt 1257493..1258296 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M8993_RS05760 (M8993_05760) - 1258302..1259153 (+) 852 WP_005751072.1 thymidylate synthase -
  M8993_RS05765 (M8993_05765) tadA 1259168..1259668 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15070.53 Da        Isoelectric Point: 9.1281

>NTDB_id=691082 M8993_RS05735 WP_250191216.1 1254344..1254778(-) (pilA) [Pasteurella multocida strain 10185]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVIIVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=691082 M8993_RS05735 WP_250191216.1 1254344..1254778(-) (pilA) [Pasteurella multocida strain 10185]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTATTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

51.678

100

0.535
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.054

100

0.368
  pilA Vibrio cholerae strain A1552

36.054

100

0.368
  pilA Vibrio cholerae C6706

36.054

100

0.368