Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8993_RS05730 Genome accession   NZ_CP097605
Coordinates   1252959..1254344 (-) Length   461 a.a.
NCBI ID   WP_169029463.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10185     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247959..1259344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8993_RS05700 (M8993_05700) - 1248085..1249458 (-) 1374 WP_169029464.1 sodium:alanine symporter family protein -
  M8993_RS05705 (M8993_05705) - 1249907..1250176 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M8993_RS05710 (M8993_05710) yacG 1250176..1250379 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M8993_RS05715 (M8993_05715) coaE 1250390..1251010 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  M8993_RS05720 (M8993_05720) - 1251025..1251747 (-) 723 WP_064968698.1 A24 family peptidase -
  M8993_RS05725 (M8993_05725) - 1251747..1252959 (-) 1213 Protein_1096 type II secretion system F family protein -
  M8993_RS05730 (M8993_05730) pilB 1252959..1254344 (-) 1386 WP_169029463.1 GspE/PulE family protein Machinery gene
  M8993_RS05735 (M8993_05735) pilA 1254344..1254778 (-) 435 WP_250191216.1 prepilin peptidase-dependent pilin Machinery gene
  M8993_RS05740 (M8993_05740) ampD 1254938..1255504 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8993_RS05745 (M8993_05745) rppH 1256090..1256686 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M8993_RS05750 (M8993_05750) - 1256689..1257483 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M8993_RS05755 (M8993_05755) lgt 1257493..1258296 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M8993_RS05760 (M8993_05760) - 1258302..1259153 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52266.01 Da        Isoelectric Point: 6.6312

>NTDB_id=691081 M8993_RS05730 WP_169029463.1 1252959..1254344(-) (pilB) [Pasteurella multocida strain 10185]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLYPPKLQVEENIPMVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSVSLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVRRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=691081 M8993_RS05730 WP_169029463.1 1252959..1254344(-) (pilB) [Pasteurella multocida strain 10185]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTACCCACCAAAATTACAAGTGGAAGAAAACATACCCATGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCGTTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACGACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-94)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.37

98.482

0.575
  pilB Acinetobacter baumannii D1279779

40.286

100

0.427
  pilB Acinetobacter baylyi ADP1

38.477

100

0.416
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.412
  pilB Vibrio cholerae strain A1552

38.477

100

0.406
  pilB Legionella pneumophila strain ERS1305867

37.423

100

0.397
  pilF Thermus thermophilus HB27

38.426

93.709

0.36