Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   M5E02_RS10215 Genome accession   NZ_CP097373
Coordinates   2024280..2024915 (+) Length   211 a.a.
NCBI ID   WP_058837936.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2019280..2029915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS10175 (M5E02_10175) - 2019429..2020547 (+) 1119 WP_252058292.1 NADH-dependent flavin oxidoreductase -
  M5E02_RS10180 (M5E02_10180) - 2020606..2021112 (-) 507 WP_095408850.1 PTS glucose transporter subunit IIA -
  M5E02_RS10185 (M5E02_10185) - 2021281..2021700 (+) 420 WP_048239363.1 Hsp20/alpha crystallin family protein -
  M5E02_RS10190 (M5E02_10190) - 2021721..2022179 (-) 459 WP_252056180.1 YppG family protein -
  M5E02_RS10195 (M5E02_10195) - 2022287..2022667 (-) 381 WP_224426683.1 YppE family protein -
  M5E02_RS10200 (M5E02_10200) - 2022717..2022950 (-) 234 WP_223251001.1 DUF5446 family protein -
  M5E02_RS10205 (M5E02_10205) sspM 2023150..2023254 (+) 105 WP_012010346.1 acid-soluble spore protein SspM -
  M5E02_RS10210 (M5E02_10210) - 2023277..2024233 (-) 957 WP_252056181.1 DUF2515 domain-containing protein -
  M5E02_RS10215 (M5E02_10215) recU 2024280..2024915 (+) 636 WP_058837936.1 Holliday junction resolvase RecU Machinery gene
  M5E02_RS10220 (M5E02_10220) - 2024935..2027646 (+) 2712 WP_252056182.1 PBP1A family penicillin-binding protein -
  M5E02_RS10225 (M5E02_10225) - 2027683..2028192 (-) 510 WP_252056183.1 YpoC family protein -
  M5E02_RS10230 (M5E02_10230) nth 2028189..2028851 (-) 663 WP_252056184.1 endonuclease III -
  M5E02_RS10235 (M5E02_10235) - 2028869..2029573 (-) 705 WP_048239354.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 211 a.a.        Molecular weight: 24315.67 Da        Isoelectric Point: 9.7504

>NTDB_id=689202 M5E02_RS10215 WP_058837936.1 2024280..2024915(+) (recU) [Bacillus safensis strain AHB2]
MIRYPNGKSYQPIQQIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKQGGICFVIISAFGSVYYLPASDLFFFWERQK
QNGRKSISKDELMEAGHIMTLGYSPRIDYIKVVETLHFSDESKQQLRSKKV

Nucleotide


Download         Length: 636 bp        

>NTDB_id=689202 M5E02_RS10215 WP_058837936.1 2024280..2024915(+) (recU) [Bacillus safensis strain AHB2]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACAAATTGGGACAAAAAAAAGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGATTTAAATGAAACGAACCAATACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCAGTTCAAATTGTGAATGTGGATTATCCAAAAAGAAGTGCTGCTGTCATTAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAACGGGGTGTACAAAGGCCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACTACCTCGTTTCCGCTCAAAAATTTCCACGACCATCAAATTGAGCATATGAAACAGGTTGAAAAACAAGGCGGTATTT
GTTTCGTTATTATATCCGCTTTTGGCTCCGTTTATTATTTGCCCGCTTCAGATCTCTTTTTCTTCTGGGAGCGGCAGAAG
CAAAACGGCCGTAAATCCATTAGCAAAGACGAATTAATGGAAGCAGGCCACATAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGCAAGCAGCAATTACGTTCGAAAAAGGTTTAA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

72.772

95.735

0.697