Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M4X66_RS00910 Genome accession   NZ_CP097286
Coordinates   201962..203662 (-) Length   566 a.a.
NCBI ID   WP_249583478.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain JACO-C-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196962..208662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4X66_RS00880 - 197458..198087 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  M4X66_RS00885 - 198212..198673 (+) 462 WP_249583475.1 hypothetical protein -
  M4X66_RS00890 yacG 199042..199251 (-) 210 WP_122439230.1 DNA gyrase inhibitor YacG -
  M4X66_RS00895 coaE 199248..199871 (-) 624 WP_249583476.1 dephospho-CoA kinase -
  M4X66_RS00900 pilD 199868..200740 (-) 873 WP_192020739.1 A24 family peptidase Machinery gene
  M4X66_RS00905 pilC 200742..201959 (-) 1218 WP_249583477.1 type II secretion system F family protein Machinery gene
  M4X66_RS00910 pilB 201962..203662 (-) 1701 WP_249583478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M4X66_RS00915 pilA2 203888..204325 (+) 438 WP_249583479.1 pilin Machinery gene
  M4X66_RS00920 - 205071..205637 (-) 567 WP_249583480.1 recombinase family protein -
  M4X66_RS00925 - 206068..207477 (+) 1410 WP_249583481.1 AAA family ATPase -
  M4X66_RS00930 - 207477..208331 (+) 855 WP_249583482.1 DUF4435 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62626.86 Da        Isoelectric Point: 6.3863

>NTDB_id=688255 M4X66_RS00910 WP_249583478.1 201962..203662(-) (pilB) [Pseudomonas viridiflava strain JACO-C-5]
MSDVVVLTGLAKQLVVAELLSEKAAQQAFQQARRDKISLISYLVQNKLVKSLTLAEMASEQFGVPFMDISSLDKESQPKG
LVSEKLARQHHALPLWRRGNRLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTAAIDKFFDSDSGLGSMADIDLEL
DIEPGEGIKETSITSRDDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKVFRVRFRTDGILHEAAKPPINLASRIAARL
KVMASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEVDVPHETLIKEGFPE
SKVGTFKLYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=688255 M4X66_RS00910 WP_249583478.1 201962..203662(-) (pilB) [Pseudomonas viridiflava strain JACO-C-5]
ATGAGTGATGTCGTTGTTCTTACCGGCCTGGCCAAGCAATTGGTCGTCGCCGAGCTTCTTTCCGAAAAAGCCGCTCAACA
GGCTTTCCAGCAGGCGCGACGCGACAAGATTTCGCTGATCAGCTATTTGGTTCAGAACAAACTGGTCAAGAGTCTCACGT
TGGCCGAAATGGCGTCCGAGCAGTTCGGCGTGCCATTCATGGACATATCAAGCCTCGATAAAGAAAGCCAGCCCAAGGGG
CTGGTAAGCGAAAAGCTCGCACGCCAGCACCACGCCCTTCCCCTATGGCGTCGGGGCAACAGACTGTTTATTGGCATCTC
GGACCCGACCAATCATCAGGCGGTGACCGACATCCAGTTCAGTACTGGTTTGAATACTGAAGCGATTCTGGTCGAAGACG
ACAAACTCACTGCAGCCATTGACAAGTTTTTTGACAGCGATAGCGGGCTCGGGTCCATGGCCGATATCGACCTGGAACTC
GATATCGAGCCGGGCGAAGGCATCAAGGAAACATCCATCACCAGCCGCGACGATGCCGATGACGCTCCGGTCGTGCGTTT
CGTCAATAAAATGCTGCTGGATGCCATTCGGCTGGGCTCGTCCGATCTGCACTTCGAACCTTACGAAAAGGTGTTTCGCG
TGCGCTTTCGTACAGACGGGATTTTGCACGAGGCGGCAAAACCTCCTATCAATCTGGCCAGTCGTATCGCGGCGCGCCTG
AAGGTCATGGCCAGCCTGGACATCTCGGAACGGCGCAAGCCTCAGGACGGCCGGGTCAAGTTGCGTGTTTCCAAAACCAA
ATCCATCGACTTCCGGATGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGATCCGACCAGCG
CCCAGATGGGCATTGATGCGCTGGGCTATGAGCCGGATCAGAAAGAGCTGTATCTGGAAGCGCTCAAGCAGCCGCAGGGC
ATGATTCTGGTGACCGGCCCGACCGGCTCGGGCAAAACCGTTTCGTTGTACACAGGCCTCAATATTCTCAACACTGTGAA
TATCAATATTTCAACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCGTCAATCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTGCGTCAGGATCCGGATGTGATCATGGTCGGTGAGATTCGCGACCTG
GAAACAGCCGAGATTGCGATCAAGGCTTCTCAGACCGGCCACATGGTGTTGTCGACGCTGCACACCAACAGCGCGGCAGA
AACGCTGACCCGCTTGCATCACATGGGCGTTGCGGCATTCAACATCGCCACGGCGATCAATCTGATCATTGCTCAGCGAC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAAGAAGTCGACGTCCCACACGAGACGCTGATCAAGGAAGGCTTCCCGGAG
TCGAAAGTCGGCACCTTCAAACTGTACGGCCCGGTCGGGTGCGAACACTGCAACGGCGGCTATCGAGGCCGAGTCGGGAT
TTACGAGGTCGTGAAAAAAACGCCGGAACTGGAACGCATTATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGA
TGCGCAAGGATGGTTTCAATGACCTGCGAACATCCGGCTTGTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.406

99.293

0.56
  pilB Acinetobacter baylyi ADP1

56.228

99.293

0.558
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.551
  pilB Vibrio cholerae strain A1552

50.267

99.117

0.498
  pilB Vibrio campbellii strain DS40M4

48.49

99.47

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.097

97.88

0.481
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479
  pilF Thermus thermophilus HB27

38.732

100

0.389