Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MLL77_RS17885 Genome accession   NZ_CP097209
Coordinates   3892126..3893832 (+) Length   568 a.a.
NCBI ID   WP_274799055.1    Uniprot ID   -
Organism   Aeromonas caviae strain W3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887126..3898832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLL77_RS17860 (MLL77_17825) pdhR 3888292..3889056 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MLL77_RS17865 (MLL77_17830) ampD 3889333..3889905 (-) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MLL77_RS17870 (MLL77_17835) - 3890034..3890504 (+) 471 WP_042015101.1 retropepsin-like aspartic protease -
  MLL77_RS17875 (MLL77_17840) nadC 3890508..3891371 (+) 864 WP_041213995.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MLL77_RS17880 (MLL77_17845) - 3891682..3892122 (+) 441 WP_274799056.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MLL77_RS17885 (MLL77_17850) pilB 3892126..3893832 (+) 1707 WP_274799055.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MLL77_RS17890 (MLL77_17855) pilC 3893960..3895201 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  MLL77_RS17895 (MLL77_17860) pilD 3895279..3896154 (+) 876 WP_182976107.1 A24 family peptidase Machinery gene
  MLL77_RS17900 (MLL77_17865) coaE 3896173..3896787 (+) 615 WP_048208563.1 dephospho-CoA kinase -
  MLL77_RS17905 (MLL77_17870) zapD 3896825..3897547 (+) 723 WP_010675825.1 cell division protein ZapD -
  MLL77_RS17910 (MLL77_17875) yacG 3897558..3897752 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  MLL77_RS17915 (MLL77_17880) mutT 3897810..3898214 (-) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=687835 MLL77_RS17885 WP_274799055.1 3892126..3893832(+) (pilB) [Aeromonas caviae strain W3]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDIIDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=687835 MLL77_RS17885 WP_274799055.1 3892126..3893832(+) (pilB) [Aeromonas caviae strain W3]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAATGACATCATCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTAGGCATGGATCATATCGACGAAGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGATGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTGAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGTGTCAACCAGGTGCAGATCAATCCCAAAG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTACTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTTACCCGGATGATGAACATGGGGGTGCCAGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCTGGTTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.24

99.12

0.548
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.12

0.496
  pilF Neisseria gonorrhoeae MS11

46.737

99.824

0.467
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37