Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M1L61_RS17710 Genome accession   NZ_CP096975
Coordinates   3793581..3795308 (-) Length   575 a.a.
NCBI ID   WP_248853872.1    Uniprot ID   -
Organism   Stenotrophomonas sp. NY11291     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3788581..3800308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1L61_RS17695 (M1L61_17695) sucC 3788840..3790009 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  M1L61_RS17700 (M1L61_17700) - 3790415..3792028 (+) 1614 WP_217014085.1 ATP-binding protein -
  M1L61_RS17705 (M1L61_17705) pilR 3792089..3793462 (+) 1374 WP_228354731.1 sigma-54 dependent transcriptional regulator Regulator
  M1L61_RS17710 (M1L61_17710) pilB 3793581..3795308 (-) 1728 WP_248853872.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M1L61_RS17715 (M1L61_17715) - 3795617..3796060 (-) 444 WP_241881160.1 pilin -
  M1L61_RS17720 (M1L61_17720) pilC 3796421..3797680 (+) 1260 WP_248853873.1 type II secretion system F family protein Machinery gene
  M1L61_RS17725 (M1L61_17725) - 3797689..3798552 (+) 864 WP_010481503.1 A24 family peptidase -
  M1L61_RS17730 (M1L61_17730) coaE 3798564..3799175 (+) 612 WP_043402827.1 dephospho-CoA kinase -
  M1L61_RS17735 (M1L61_17735) - 3799336..3799722 (-) 387 WP_049481722.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62209.20 Da        Isoelectric Point: 6.0426

>NTDB_id=685837 M1L61_RS17710 WP_248853872.1 3793581..3795308(-) (pilB) [Stenotrophomonas sp. NY11291]
MNAVTTPNLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPEHA
LLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=685837 M1L61_RS17710 WP_248853872.1 3793581..3795308(-) (pilB) [Stenotrophomonas sp. NY11291]
ATGAATGCGGTAACCACCCCCAATCTCGTCGGCATCACCGGACTGGCCCGCCGCCTGGTCCAGGACGGTGCCCTGGATGA
GGCCTCTGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCTCGCCAGCCGCTGCCAACCTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTCGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCCCTGTTCGACGTTTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAATCCGACCCATGCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTAGAACCGATCCTGG
TCGACGAAGACCAGATCCGCCGGACGCTGGAGCAGTGGCACGCCAGCCACGACACCATCGGCGACGCGCTCGGTGGCGAT
GACGAGGGCATGGCCAACCTCGATGTCGGGTTGGGTGACGAGGACAATGCCGGCGGCGACTCCGGCATCGACGCCAAGGG
TGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCAAGGGCGCATCGGACATCCACTTCG
AACCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGTCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAGGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTGTACACGGC
GCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGTGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCCCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCGCAGCGCCTGGCACGCCGGTTGTGCGGCAACTGCAAGCGTCCGGCCAACCTGCCGGAGCATGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGTACGAGGCGGTGGGCTGCGATGAGTGCAC
CGAGGGCTACAAGGGCCGAACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACCATCGTGCTGGCCGGTG
GCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGAGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCT
GCGGGCGTGACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.401

100

0.567
  pilB Acinetobacter baumannii D1279779

56.62

99.826

0.565
  pilB Legionella pneumophila strain ERS1305867

51.664

99.304

0.513
  pilB Vibrio cholerae strain A1552

48.776

99.478

0.485
  pilF Neisseria gonorrhoeae MS11

49.206

98.609

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.235

99.304

0.459
  pilB Vibrio campbellii strain DS40M4

44.991

98.957

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.17

100

0.384
  pilF Thermus thermophilus HB27

37.766

98.087

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

93.043

0.363