Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OK117_RS11145 Genome accession   NZ_CP109886
Coordinates   2405516..2405962 (+) Length   148 a.a.
NCBI ID   WP_057682388.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. fastidiosa strain CFBP8073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2400516..2410962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK117_RS11125 (OK117_11135) - 2400764..2402101 (+) 1338 WP_004090387.1 HAMP domain-containing sensor histidine kinase -
  OK117_RS11130 (OK117_11140) coaE 2402368..2402991 (-) 624 WP_020851236.1 dephospho-CoA kinase -
  OK117_RS11135 (OK117_11145) pilD 2403003..2403866 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  OK117_RS11140 (OK117_11150) pilC 2403873..2405075 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  OK117_RS11145 (OK117_11155) pilA 2405516..2405962 (+) 447 WP_057682388.1 pilin Machinery gene
  OK117_RS11150 (OK117_11160) - 2406210..2406437 (-) 228 WP_058564297.1 hypothetical protein -
  OK117_RS11155 (OK117_11165) - 2406837..2407136 (-) 300 WP_155561232.1 hypothetical protein -
  OK117_RS11160 (OK117_11170) pilA/pilAI 2407280..2407708 (+) 429 WP_306308946.1 pilin Machinery gene
  OK117_RS11165 (OK117_11175) pilB 2408564..2410297 (+) 1734 WP_058564300.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15372.58 Da        Isoelectric Point: 8.4988

>NTDB_id=681114 OK117_RS11145 WP_057682388.1 2405516..2405962(+) (pilA) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKATSNASDIGLRTDTTRCGI
TVKVEAAGTANITCKIKGNSQVSDKTIAWDRTPDNSAGTNGVNNGGVWTCSTTVTSDALRPSGCIASK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=681114 OK117_RS11145 WP_057682388.1 2405516..2405962(+) (pilA) [Xylella fastidiosa subsp. fastidiosa strain CFBP8073]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGCTACATCCAACGCGAGTGATATCGGTCTGCGTACTGACACCACTCGTTGTGGAATT
ACCGTTAAAGTCGAGGCCGCTGGCACTGCCAACATCACATGCAAAATTAAGGGCAACTCTCAAGTCAGTGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCACCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCTCTAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40

100

0.419
  pilA Vibrio cholerae strain A1552

40

100

0.419
  pilA Vibrio cholerae C6706

40

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.497

99.324

0.412
  pilA Pseudomonas aeruginosa PAK

37.736

100

0.405
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.816

99.324

0.405
  pilA Acinetobacter baumannii strain A118

38

100

0.385
  pilA Vibrio parahaemolyticus RIMD 2210633

36.486

100

0.365