Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M0D47_RS18160 Genome accession   NZ_CP096140
Coordinates   4274096..4275226 (+) Length   376 a.a.
NCBI ID   WP_101361619.1    Uniprot ID   A0A2N3RMW3
Organism   Xanthomonas prunicola strain MAI5037     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4269096..4280226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D47_RS18130 (M0D47_18115) - 4269254..4269895 (-) 642 WP_260807434.1 M23 family metallopeptidase -
  M0D47_RS18135 (M0D47_18120) - 4270001..4270426 (-) 426 WP_101361623.1 HU family DNA-binding protein -
  M0D47_RS18140 (M0D47_18125) - 4270567..4270827 (-) 261 WP_260807435.1 hypothetical protein -
  M0D47_RS18145 (M0D47_18130) proC 4270973..4271833 (-) 861 WP_252162486.1 pyrroline-5-carboxylate reductase -
  M0D47_RS18150 (M0D47_18135) - 4271879..4272571 (-) 693 WP_252162487.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0D47_RS18155 (M0D47_18140) pilT 4272936..4273973 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  M0D47_RS18160 (M0D47_18145) pilU 4274096..4275226 (+) 1131 WP_101361619.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0D47_RS18165 (M0D47_18150) - 4275538..4276212 (+) 675 WP_252165121.1 YitT family protein -
  M0D47_RS18170 (M0D47_18155) - 4276501..4276782 (-) 282 Protein_3536 DUF72 domain-containing protein -
  M0D47_RS18175 (M0D47_18160) - 4276819..4277391 (-) 573 WP_260807437.1 DNA-3-methyladenine glycosylase I -
  M0D47_RS18180 (M0D47_18165) - 4277388..4279232 (-) 1845 WP_252162489.1 DUF4153 domain-containing protein -
  M0D47_RS18185 (M0D47_18170) - 4279308..4279874 (+) 567 WP_252162490.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41975.20 Da        Isoelectric Point: 6.7683

>NTDB_id=679645 M0D47_RS18160 WP_101361619.1 4274096..4275226(+) (pilU) [Xanthomonas prunicola strain MAI5037]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQSPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=679645 M0D47_RS18160 WP_101361619.1 4274096..4275226(+) (pilU) [Xanthomonas prunicola strain MAI5037]
ATGAGCACCATCGATTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTCAATGGCAAGATCAGCCCGATCACCCAGAGCCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGA
GTCGGACGTTTTCGTGTGAGCTGTTTTTACCAGCGCAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTTGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAATTCCACTGGCCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATTGCCTTCGCTGAAACCGGCCACTTGGTGTTGTGCACGCTGCACGCCAACAACGCCAACCAAGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGTCGTAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGTAGCCGCCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAAAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGTTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAACTGTCGCAAGGCGGCGATGCCAAAACCTTGGCGCAGGGCCTGGATGGTGTGGAAATTTCCG
AGGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N3RMW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362