Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M0D45_RS04115 Genome accession   NZ_CP096139
Coordinates   999547..1000677 (-) Length   376 a.a.
NCBI ID   WP_101361619.1    Uniprot ID   A0A2N3RMW3
Organism   Xanthomonas prunicola strain MAI5069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 994547..1005677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D45_RS04090 (M0D45_04080) - 994900..995466 (-) 567 WP_252162490.1 YqgE/AlgH family protein -
  M0D45_RS04095 (M0D45_04085) - 995542..997386 (+) 1845 WP_260817635.1 DUF4153 domain-containing protein -
  M0D45_RS04100 (M0D45_04090) - 997383..997955 (+) 573 WP_260817636.1 DNA-3-methyladenine glycosylase I -
  M0D45_RS04105 (M0D45_04095) - 997992..998272 (+) 281 Protein_794 DUF72 domain-containing protein -
  M0D45_RS04110 (M0D45_04100) - 998561..999235 (-) 675 WP_252165121.1 YitT family protein -
  M0D45_RS04115 (M0D45_04105) pilU 999547..1000677 (-) 1131 WP_101361619.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0D45_RS04120 (M0D45_04110) pilT 1000800..1001837 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  M0D45_RS04125 (M0D45_04115) - 1002202..1002894 (+) 693 WP_252162487.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0D45_RS04130 (M0D45_04120) proC 1002940..1003800 (+) 861 WP_252162486.1 pyrroline-5-carboxylate reductase -
  M0D45_RS04135 (M0D45_04125) - 1004345..1004770 (+) 426 WP_101361623.1 HU family DNA-binding protein -
  M0D45_RS04140 (M0D45_04130) - 1004878..1005519 (+) 642 WP_260817637.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41975.20 Da        Isoelectric Point: 6.7683

>NTDB_id=679612 M0D45_RS04115 WP_101361619.1 999547..1000677(-) (pilU) [Xanthomonas prunicola strain MAI5069]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQSPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=679612 M0D45_RS04115 WP_101361619.1 999547..1000677(-) (pilU) [Xanthomonas prunicola strain MAI5069]
ATGAGCACCATCGATTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTCAATGGCAAGATCAGCCCGATCACCCAGAGCCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGA
GTCGGGCGTTTTCGTGTGAGCTGTTTTTACCAGCGCAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTTGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAATTCCACTGGCCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCA
TGGACCACGCGATTGCCTTCGCTGAAACCGGCCACTTGGTGTTGTGCACGCTGCACGCCAACAACGCCAACCAAGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGTCGTAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGTAGCCGCCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAAAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGTTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAACTGTCGCAAGGCGGCGATGCCAAAACCTTGGCGCAGGGCCTGGATGGTGTGGAAATTTCCG
AGGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N3RMW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362