Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MZO21_RS11240 Genome accession   NZ_CP096120
Coordinates   2256890..2258602 (+) Length   570 a.a.
NCBI ID   WP_154772008.1    Uniprot ID   -
Organism   Acinetobacter portensis strain JNE5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251890..2263602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZO21_RS11195 (MZO21_11190) nusA 2252505..2253989 (-) 1485 WP_154772012.1 transcription termination factor NusA -
  MZO21_RS11200 (MZO21_11195) rimP 2254025..2254549 (-) 525 WP_154772011.1 ribosome maturation factor RimP -
  MZO21_RS11230 (MZO21_11225) secG 2255477..2255806 (-) 330 WP_154772010.1 preprotein translocase subunit SecG -
  MZO21_RS11235 (MZO21_11230) tpiA 2255820..2256611 (-) 792 WP_154772009.1 triose-phosphate isomerase -
  MZO21_RS11240 (MZO21_11235) pilB 2256890..2258602 (+) 1713 WP_154772008.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MZO21_RS11245 (MZO21_11240) pilC 2258632..2259858 (+) 1227 WP_248103368.1 type II secretion system F family protein Machinery gene
  MZO21_RS15125 pilD 2259858..2260724 (+) 867 WP_282441558.1 A24 family peptidase Machinery gene
  MZO21_RS11260 (MZO21_11255) coaE 2260727..2261332 (+) 606 WP_248103370.1 dephospho-CoA kinase -
  MZO21_RS11265 (MZO21_11260) - 2261329..2262249 (-) 921 WP_248103375.1 DMT family transporter -
  MZO21_RS11270 (MZO21_11265) rlmB 2262269..2263018 (-) 750 WP_248103377.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MZO21_RS11275 (MZO21_11270) - 2263151..2263474 (-) 324 WP_154772002.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63390.58 Da        Isoelectric Point: 4.7725

>NTDB_id=679577 MZO21_RS11240 WP_154772008.1 2256890..2258602(+) (pilB) [Acinetobacter portensis strain JNE5]
MSVIQSTPKFTGFIRRLVENGVVDATSMQNAIINAKKANQDIIPYLTQNLKVSSLTIAETIALEFGEPIFDLSVYNTDML
AKDAVDEKLIIKHRVLPIFNRGNLLYVATSNPTNIEAIDAIRFNSKMNVESVIVEFDKLDKLIENLFADTGNFEFSDDEF
DLDVEEVDPNQENQKDEDPTGDESPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKIGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSHCKAPADIPEQSLLELGF
TTEDLADPETKIYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASEKAGFRNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=679577 MZO21_RS11240 WP_154772008.1 2256890..2258602(+) (pilB) [Acinetobacter portensis strain JNE5]
ATGTCAGTCATACAATCTACGCCGAAATTCACCGGTTTTATTCGACGCCTTGTTGAAAATGGGGTTGTTGATGCTACATC
AATGCAAAATGCCATCATCAATGCAAAAAAAGCCAATCAAGATATCATTCCTTATTTGACCCAAAATTTAAAAGTTTCTT
CACTCACCATTGCTGAGACTATTGCTTTAGAATTTGGTGAACCTATTTTCGACTTATCTGTATACAACACAGATATGCTC
GCAAAAGATGCTGTTGATGAAAAACTTATAATAAAACATCGTGTTTTACCTATTTTTAACCGAGGTAATCTTCTATATGT
GGCAACCAGTAATCCCACAAATATAGAAGCGATAGATGCCATTCGCTTTAACAGTAAAATGAATGTTGAATCTGTTATTG
TCGAGTTTGATAAACTAGATAAACTTATTGAAAATTTATTTGCAGATACAGGGAACTTTGAGTTTAGTGATGATGAGTTC
GATTTAGATGTTGAAGAAGTTGATCCTAATCAAGAAAATCAAAAAGATGAAGACCCAACAGGTGATGAATCTCCAATTGT
AAAATATATCAATAAACTCTTAATTGATGCTATTCGTATGGGCGCTTCAGATTTACACTTTGAACCCTATGAAAAATCTT
ATCGTGTTCGATACCGTGTAGATGGTGTATTACGTCAAATTGCAACTCCCCCACTACAGTTAGCAAACCGTTTAGCATCA
CGTTTAAAAGTAATGTCACAAATGGACATTTCAGAAAAACGTGTTCCGCAAGATGGTCGTATTAAATTAAAAATCTCAAA
AACAAAAGCTATCGACTTCCGTGTTAACTCACTCCCAACCTTATTTGGTGAGAAACTCGTACTTCGTATTTTAGATCCAT
CTAGTGCGATGTTGGGTATTGATGCTTTGGGTTATGAGCCTGATCAAAAAGAACTTTTCATGGATGCACTTGATAAACCT
CAAGGCATGCTATTAATTACAGGTCCTACTGGTTCGGGTAAAACGGTATCTTTATATACAGGTTTGAATATTCTAAACCG
TGAAGATACCAATATTTCAACAGCCGAAGATCCTGTCGAAATTAACTTAGAAGGTATTAACCAAGTTAACGTTAATAATA
AAATTGGTTTAACCTTCTCTGCGGCACTTAAATCATTTCTTCGTCAAGATCCAGATATTGTTATGGTCGGGGAAATTCGT
GACCTTGAAACAGCTGAAATTGCAATTAAAGCAGCACAAACAGGTCATATGGTAATGTCCACACTACATACAAACAGCGC
ACCTGAAACATTAACGCGCTTAAGAAATATGGGCGTTCAATCTTTCAATATTGCAACATCTGTTAACCTTGTTATTGCAC
AACGTTTAGCACGTCGTTTATGCTCCCACTGCAAAGCTCCTGCCGACATTCCAGAACAAAGCTTATTAGAACTAGGATTC
ACCACAGAAGACTTAGCTGATCCAGAAACTAAAATTTATCAACCTGTAGGCTGTTCTGAGTGTCGTGAAGGTTATAAAGG
ACGTGTAGGTATTTATGAGGTAATGAAAGTTACACCAGAAATTTCAAAAATTATTATGGAAGATGGTAATGCACTTCAAA
TTGCAGAAGCCTCTGAAAAAGCAGGTTTTAGAAACTTACGCCGTTCTGGTTTAATAAAAGTCATGCAAGGGGTAACTTCT
TTACAAGAAGTAAATCGTGTGACGAGTGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.825

100

0.798
  pilB Acinetobacter baylyi ADP1

76.708

100

0.768
  pilB Legionella pneumophila strain ERS1305867

54.034

93.509

0.505
  pilF Neisseria gonorrhoeae MS11

50.89

98.596

0.502
  pilB Vibrio cholerae strain A1552

49.296

99.649

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.91

97.368

0.486
  pilB Vibrio campbellii strain DS40M4

50.672

91.404

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

35.113

100

0.381
  pilF Thermus thermophilus HB27

36.87

100

0.372