Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MYA84_RS01215 Genome accession   NZ_CP095872
Coordinates   260021..261730 (-) Length   569 a.a.
NCBI ID   WP_048881746.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain H4-3-C1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 255021..266730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYA84_RS01185 (MYA84_01180) - 255128..255451 (+) 324 WP_004810087.1 pyrimidine/purine nucleoside phosphorylase -
  MYA84_RS01190 (MYA84_01185) rlmB 255604..256353 (+) 750 WP_004810085.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MYA84_RS01195 (MYA84_01190) - 256360..257283 (+) 924 WP_081405839.1 DMT family transporter -
  MYA84_RS01200 (MYA84_01195) coaE 257299..257898 (-) 600 WP_048881745.1 dephospho-CoA kinase -
  MYA84_RS01205 pilD 257900..258760 (-) 861 WP_004810080.1 prepilin peptidase Machinery gene
  MYA84_RS01210 (MYA84_01210) pilC 258760..259989 (-) 1230 WP_004810078.1 type II secretion system F family protein Machinery gene
  MYA84_RS01215 (MYA84_01215) pilB 260021..261730 (-) 1710 WP_048881746.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MYA84_RS01220 (MYA84_01220) tpiA 262013..262804 (+) 792 WP_184103376.1 triose-phosphate isomerase -
  MYA84_RS01225 (MYA84_01225) secG 262818..263147 (+) 330 WP_004810073.1 preprotein translocase subunit SecG -
  MYA84_RS01255 (MYA84_01255) rimP 264209..264733 (+) 525 WP_004896325.1 ribosome maturation factor RimP -
  MYA84_RS01260 (MYA84_01260) nusA 264769..266253 (+) 1485 WP_004810069.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63550.73 Da        Isoelectric Point: 4.8758

>NTDB_id=678936 MYA84_RS01215 WP_048881746.1 260021..261730(-) (pilB) [Acinetobacter schindleri strain H4-3-C1]
MTAIQGSPRFTGFIRRLVDEGVISAEDMQGALSSAKKDKIDIVPYLIEHHRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNSNILFIATSNPTNIEAIDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDIDNSDPNKDDEDDNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKPADIPRQSLLEMGFS
DDDLQNPEFNIFEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=678936 MYA84_RS01215 WP_048881746.1 260021..261730(-) (pilB) [Acinetobacter schindleri strain H4-3-C1]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGAGTTATATCTGCAGAAGA
TATGCAGGGTGCTTTGAGCAGTGCCAAAAAAGATAAAATTGATATTGTTCCCTATCTGATTGAACATCACCGCCTCTCGC
CAACCAAAATCGCAGAAACGATTTCTCTTGAATTCGGTGAACCGCTATTTGATATCAGTGCCTATGATCCTGCCCTGATC
GTACGCGAAGATTTTGATGAAAAGATCATTACCAAACACCGCGTGCTGCCAATCTTTAAAAATAGCAATATTCTGTTTAT
CGCGACCAGCAATCCGACCAATATCGAAGCAATTGATGCTGTCCGTTTTGCGACCAAACTCAATATTGAAACCATCATTG
TTGAACATAATAAACTCGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGACTTTGATGATGATTTTGAT
CTAGATGTTGATATCGATAACTCTGACCCGAATAAAGATGATGAGGATGACAATAAAGGTGATGAAGCACCCATTGTTAA
ATATATTAATAAGTTACTGATTGATGCAATCCGTATGGGTGCATCTGATTTACACTTTGAACCTTATGAAAAAATGTACC
GTGTGCGTTACCGTGTCGACGGGGTCTTGCGTCAAATCGCCACGCCACCTTTACAGCTTGCAACACGTTTGGCTTCACGT
CTTAAAGTTATGTCACAAATGGATATTTCCGAGAAGCGTGTGCCTCAAGATGGCCGGATCAAGTTAAAACTCTCTAAAAA
TAAGGCTATCGATTTCCGTGTCAACTCACTCCCCACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATCCATCGA
GTGCCATGCTTGGAATTGATGCTTTGGGGTATGAGCCTGATCAAAAAGCCTTATTTATGGAGGCACTAGATAAGCCTCAA
GGTATGCTGTTAATTACCGGTCCGACAGGCTCTGGTAAAACTGTATCTCTTTATACCGGTTTAAATATCCTGAATCGTGA
AGACACCAATATTTCAACTGCTGAAGATCCAGTTGAAATTAACTTGCAAGGGATCAATCAGGTTAACGTCAATAATAAAG
TTGGCCTAACCTTCTCTGCTGCATTGAAATCATTCCTGCGCCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAC
CTGGAAACTGCAGAGATTGCAATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACACTACATACCAACAGCGCACC
AGAAACCCTGACCCGTTTACGTAATATGGGTGTGCCATCATTTAACATTGCCACCTCGGTGAATTTGGTTATTGCTCAGC
GTTTGGCGCGTCGCCTTTGTCCACATTGCAAAAAACCGGCAGATATTCCACGTCAGAGCCTGCTTGAAATGGGCTTTAGC
GATGATGATTTACAGAATCCGGAATTTAATATTTTTGAACCTGTCGGATGTAACGAATGTCGTGAAGGGTATAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCAGAGATTTCACGTATTATTATGGAAGATGGCAATGCAATTGAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.037

94.903

0.494
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

97.891

0.483
  pilB Vibrio cholerae strain A1552

48.501

99.649

0.483
  pilB Vibrio campbellii strain DS40M4

48.115

97.891

0.471
  pilF Thermus thermophilus HB27

37.173

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.374