Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MYF86_RS02345 Genome accession   NZ_CP095841
Coordinates   492004..493710 (+) Length   568 a.a.
NCBI ID   WP_139463613.1    Uniprot ID   -
Organism   Aeromonas veronii strain AV040     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487004..498710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF86_RS02320 (MYF86_02320) pdhR 488144..488908 (-) 765 WP_159144446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MYF86_RS02325 (MYF86_02325) ampD 489187..489762 (-) 576 WP_247823576.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MYF86_RS02330 (MYF86_02330) - 489893..490363 (+) 471 WP_040068932.1 retropepsin-like aspartic protease -
  MYF86_RS02335 (MYF86_02335) nadC 490367..491242 (+) 876 WP_019444781.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MYF86_RS02340 (MYF86_02340) - 491558..492001 (+) 444 WP_247823577.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MYF86_RS02345 (MYF86_02345) pilB 492004..493710 (+) 1707 WP_139463613.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MYF86_RS02350 (MYF86_02350) pilC 493950..495191 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  MYF86_RS02355 (MYF86_02355) pilD 495253..496125 (+) 873 WP_247823578.1 A24 family peptidase Machinery gene
  MYF86_RS02360 (MYF86_02360) coaE 496139..496753 (+) 615 WP_247823579.1 dephospho-CoA kinase -
  MYF86_RS02365 (MYF86_02365) zapD 496791..497513 (+) 723 WP_005355104.1 cell division protein ZapD -
  MYF86_RS02370 (MYF86_02370) yacG 497523..497717 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  MYF86_RS02375 (MYF86_02375) mutT 497795..498199 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62519.85 Da        Isoelectric Point: 5.3170

>NTDB_id=678683 MYF86_RS02345 WP_139463613.1 492004..493710(+) (pilB) [Aeromonas veronii strain AV040]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDDNSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=678683 MYF86_RS02345 WP_139463613.1 492004..493710(+) (pilB) [Aeromonas veronii strain AV040]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTAGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAGGTCTCCGACGATAACAGCCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTACGCTTTCGCATCGATGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAGCGGCGGTTGCCGCAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTTAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAATCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACTGCCGAAATCGCCATCAAGGCAGCACAAACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCGGCTGC
CGAAACCCTGACCCGAATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCAGCCGGGCTTAGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGCGGCTACAAAGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CGATCGCCCAGAAGGAAGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.722

95.599

0.542
  pilB Vibrio cholerae strain A1552

54.937

98.063

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.241

97.359

0.382
  pilF Thermus thermophilus HB27

40.53

92.958

0.377