Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NALG_RS05160 Genome accession   NZ_AP017373
Coordinates   1057766..1058662 (-) Length   298 a.a.
NCBI ID   WP_231951199.1    Uniprot ID   -
Organism   Lactococcus formosensis strain 122061     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1059201..1088954 1057766..1058662 flank 539


Gene organization within MGE regions


Location: 1057766..1088954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NALG_RS05160 (NALG_1065) comGA 1057766..1058662 (-) 897 WP_231951199.1 competence type IV pilus ATPase ComGA Machinery gene
  NALG_RS05165 (NALG_1066) rpsU 1058896..1059072 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  NALG_RS05170 (NALG_1067) - 1059201..1060862 (-) 1662 WP_096369379.1 S-layer homology domain-containing protein -
  NALG_RS05175 (NALG_1068) guaB 1061218..1062699 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  NALG_RS05180 (NALG_1069) - 1062873..1064477 (+) 1605 WP_096368801.1 GH25 family lysozyme -
  NALG_RS05185 (NALG_1070) - 1064507..1065874 (-) 1368 WP_096368802.1 DUF2142 domain-containing protein -
  NALG_RS05190 (NALG_1071) - 1065884..1067551 (-) 1668 WP_231951076.1 surface protein -
  NALG_RS05195 (NALG_1072) tagD 1067636..1068049 (-) 414 WP_096368804.1 glycerol-3-phosphate cytidylyltransferase -
  NALG_RS05200 (NALG_1073) - 1068074..1068961 (-) 888 WP_096368805.1 glycosyltransferase family A protein -
  NALG_RS05205 (NALG_1074) - 1068963..1069955 (-) 993 WP_096368806.1 glycosyltransferase -
  NALG_RS05210 (NALG_1075) - 1069976..1070800 (-) 825 WP_096368807.1 phosphorylcholine transferase LicD -
  NALG_RS05215 (NALG_1076) - 1070797..1071936 (-) 1140 WP_185767487.1 glycosyltransferase family 4 protein -
  NALG_RS05220 (NALG_1077) - 1071929..1073362 (-) 1434 WP_096368809.1 lipopolysaccharide biosynthesis protein -
  NALG_RS05225 (NALG_1078) - 1073359..1074303 (-) 945 WP_096368810.1 NAD-dependent epimerase/dehydratase family protein -
  NALG_RS05230 (NALG_1079) - 1074290..1074640 (-) 351 WP_017368733.1 DUF2304 domain-containing protein -
  NALG_RS05235 (NALG_1080) - 1074648..1075349 (-) 702 WP_096368811.1 glycosyltransferase family 2 protein -
  NALG_RS05240 (NALG_1081) - 1075448..1076278 (-) 831 WP_096368812.1 glycosyltransferase -
  NALG_RS05245 (NALG_1082) - 1076281..1077525 (-) 1245 WP_096368813.1 glycosyltransferase family 4 protein -
  NALG_RS05250 (NALG_1083) - 1077609..1080545 (-) 2937 WP_096368814.1 rhamnan synthesis F family protein -
  NALG_RS05255 (NALG_1084) - 1080565..1081899 (-) 1335 WP_096368815.1 DUF2142 domain-containing protein -
  NALG_RS05260 (NALG_1085) - 1081909..1082799 (-) 891 WP_096368816.1 polysaccharide pyruvyl transferase family protein -
  NALG_RS05265 (NALG_1086) - 1082796..1083956 (-) 1161 WP_096368817.1 ABC transporter ATP-binding protein -
  NALG_RS05270 (NALG_1087) - 1083967..1084776 (-) 810 WP_096368818.1 ABC transporter permease -
  NALG_RS05275 (NALG_1088) - 1084773..1085714 (-) 942 WP_096368819.1 glycosyltransferase family 2 protein -
  NALG_RS05280 (NALG_1089) - 1085736..1086887 (-) 1152 WP_096368820.1 glycosyltransferase family 1 protein -
  NALG_RS05285 (NALG_1090) rfbD 1086999..1087895 (-) 897 WP_096368821.1 dTDP-4-dehydrorhamnose reductase -
  NALG_RS05290 (NALG_1091) rfbB 1087911..1088954 (-) 1044 WP_017368721.1 dTDP-glucose 4,6-dehydratase -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34010.79 Da        Isoelectric Point: 6.1694

>NTDB_id=67818 NALG_RS05160 WP_231951199.1 1057766..1058662(-) (comGA) [Lactococcus formosensis strain 122061]
MVHDLYLVALEGRYSLYFRTATERRFEEEVSLEQGQALIAHMKFLSGMNLGESRRVQLGACTYSLDKGKQRLRLSTVGDF
HGQESMVIRFLHHQQNQLYFWNSELFNTFMGGRGLYLFSGPVGSGKTSLMYKFAREYFKDQQVICIEDPVELVEAEFLQL
QVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVVARLKELGLTDWELQSSLQ
RVIYQRLIAGKGLFVCEKEKFEEWQPDEWNGQIDQLVADGFISAATAAREKIEFSEAD

Nucleotide


Download         Length: 897 bp        

>NTDB_id=67818 NALG_RS05160 WP_231951199.1 1057766..1058662(-) (comGA) [Lactococcus formosensis strain 122061]
ATGGTGCATGATCTTTACTTAGTAGCATTAGAAGGGAGGTATTCTCTATATTTTCGTACAGCAACAGAAAGGAGATTTGA
AGAAGAAGTATCCCTTGAACAAGGGCAAGCTCTGATCGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTA
GACGGGTACAGTTGGGGGCTTGCACTTATAGCTTAGATAAAGGTAAGCAACGCTTACGTTTATCTACTGTCGGGGATTTT
CATGGTCAAGAAAGTATGGTTATTCGATTCTTGCATCATCAACAAAACCAACTTTATTTTTGGAACTCAGAGCTCTTTAA
CACTTTTATGGGTGGAAGAGGACTTTATCTGTTTTCAGGACCTGTGGGGTCAGGTAAGACCAGCCTTATGTACAAGTTTG
CAAGAGAGTATTTTAAGGATCAACAAGTTATTTGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTT
CAAGTCAATAAAGTTATAGGGAATGATTACGATGCCTTGATTAAACTTTCTCTTCGCCATAGACCTGATTTACTCATTGT
TGGTGAAATTAGGGACAGTCAGACGGCTAAGGCTGTGCTTCGTGCAAGCTTGACTGGGTATACGGTGTTCTCCACTGTTC
ATGCACGCTCAATATCGGGTGTAGTTGCACGCCTAAAAGAACTTGGATTAACTGATTGGGAACTACAATCCAGTCTTCAG
CGAGTGATTTATCAGCGATTAATTGCAGGAAAGGGATTATTTGTTTGTGAAAAAGAAAAGTTTGAAGAATGGCAACCAGA
CGAATGGAATGGCCAGATTGATCAGTTGGTTGCAGATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGT
TTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(3-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

61.017

98.993

0.604
  comYA Streptococcus gordonii str. Challis substr. CH1

54.882

99.664

0.547
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.741

98.658

0.53
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.401

98.658

0.527
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.881

98.993

0.523
  comYA Streptococcus mutans UA159

52.721

98.658

0.52
  comYA Streptococcus mutans UA140

52.721

98.658

0.52

Multiple sequence alignment