Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NALG_RS05160 Genome accession   NZ_AP017373
Coordinates   1057766..1058662 (-) Length   298 a.a.
NCBI ID   WP_231951199.1    Uniprot ID   -
Organism   Lactococcus formosensis strain 122061     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1052766..1063662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NALG_RS05125 (NALG_1059) - 1053371..1054240 (+) 870 WP_096368795.1 RluA family pseudouridine synthase -
  NALG_RS05130 (NALG_1060) - 1054327..1055172 (-) 846 WP_096368796.1 zinc ABC transporter substrate-binding protein -
  NALG_RS05135 (NALG_1061) comGF 1055407..1055847 (-) 441 WP_096368797.1 competence type IV pilus minor pilin ComGF -
  NALG_RS10385 comGE 1055828..1056061 (-) 234 WP_269457349.1 competence type IV pilus minor pilin ComGE -
  NALG_RS10390 comGD 1056093..1056509 (-) 417 WP_096368799.1 competence type IV pilus minor pilin ComGD Machinery gene
  NALG_RS05150 (NALG_1063) comYC 1056484..1056789 (-) 306 WP_096368800.1 competence type IV pilus major pilin ComGC Machinery gene
  NALG_RS05155 (NALG_1064) comGB 1056808..1057827 (-) 1020 WP_269457350.1 competence type IV pilus assembly protein ComGB Machinery gene
  NALG_RS05160 (NALG_1065) comGA 1057766..1058662 (-) 897 WP_231951199.1 competence type IV pilus ATPase ComGA Machinery gene
  NALG_RS05165 (NALG_1066) rpsU 1058896..1059072 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  NALG_RS05170 (NALG_1067) - 1059201..1060862 (-) 1662 WP_096369379.1 S-layer homology domain-containing protein -
  NALG_RS05175 (NALG_1068) guaB 1061218..1062699 (-) 1482 WP_003133714.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34010.79 Da        Isoelectric Point: 6.1694

>NTDB_id=67818 NALG_RS05160 WP_231951199.1 1057766..1058662(-) (comGA) [Lactococcus formosensis strain 122061]
MVHDLYLVALEGRYSLYFRTATERRFEEEVSLEQGQALIAHMKFLSGMNLGESRRVQLGACTYSLDKGKQRLRLSTVGDF
HGQESMVIRFLHHQQNQLYFWNSELFNTFMGGRGLYLFSGPVGSGKTSLMYKFAREYFKDQQVICIEDPVELVEAEFLQL
QVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVVARLKELGLTDWELQSSLQ
RVIYQRLIAGKGLFVCEKEKFEEWQPDEWNGQIDQLVADGFISAATAAREKIEFSEAD

Nucleotide


Download         Length: 897 bp        

>NTDB_id=67818 NALG_RS05160 WP_231951199.1 1057766..1058662(-) (comGA) [Lactococcus formosensis strain 122061]
ATGGTGCATGATCTTTACTTAGTAGCATTAGAAGGGAGGTATTCTCTATATTTTCGTACAGCAACAGAAAGGAGATTTGA
AGAAGAAGTATCCCTTGAACAAGGGCAAGCTCTGATCGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTA
GACGGGTACAGTTGGGGGCTTGCACTTATAGCTTAGATAAAGGTAAGCAACGCTTACGTTTATCTACTGTCGGGGATTTT
CATGGTCAAGAAAGTATGGTTATTCGATTCTTGCATCATCAACAAAACCAACTTTATTTTTGGAACTCAGAGCTCTTTAA
CACTTTTATGGGTGGAAGAGGACTTTATCTGTTTTCAGGACCTGTGGGGTCAGGTAAGACCAGCCTTATGTACAAGTTTG
CAAGAGAGTATTTTAAGGATCAACAAGTTATTTGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTT
CAAGTCAATAAAGTTATAGGGAATGATTACGATGCCTTGATTAAACTTTCTCTTCGCCATAGACCTGATTTACTCATTGT
TGGTGAAATTAGGGACAGTCAGACGGCTAAGGCTGTGCTTCGTGCAAGCTTGACTGGGTATACGGTGTTCTCCACTGTTC
ATGCACGCTCAATATCGGGTGTAGTTGCACGCCTAAAAGAACTTGGATTAACTGATTGGGAACTACAATCCAGTCTTCAG
CGAGTGATTTATCAGCGATTAATTGCAGGAAAGGGATTATTTGTTTGTGAAAAAGAAAAGTTTGAAGAATGGCAACCAGA
CGAATGGAATGGCCAGATTGATCAGTTGGTTGCAGATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGT
TTAGCGAAGCAGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(3-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

61.017

98.993

0.604
  comYA Streptococcus gordonii str. Challis substr. CH1

54.882

99.664

0.547
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.741

98.658

0.53
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.401

98.658

0.527
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.401

98.658

0.527
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.881

98.993

0.523
  comYA Streptococcus mutans UA159

52.721

98.658

0.52
  comYA Streptococcus mutans UA140

52.721

98.658

0.52

Multiple sequence alignment