Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRK42_RS19235 Genome accession   NZ_CP095328
Coordinates   4190535..4192241 (+) Length   568 a.a.
NCBI ID   WP_354688550.1    Uniprot ID   A0AAU6T7K1
Organism   Aeromonas sp. 19NY04SH05-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4185535..4197241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK42_RS19210 (MRK42_19155) pdhR 4186779..4187543 (-) 765 WP_026456603.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MRK42_RS19215 (MRK42_19160) ampD 4187810..4188391 (-) 582 WP_354688546.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MRK42_RS19220 (MRK42_19165) - 4188415..4188885 (+) 471 WP_354688547.1 retropepsin-like aspartic protease -
  MRK42_RS19225 (MRK42_19170) nadC 4188889..4189746 (+) 858 WP_354688548.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MRK42_RS19230 (MRK42_19175) - 4190121..4190531 (+) 411 WP_354688549.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MRK42_RS19235 (MRK42_19180) pilB 4190535..4192241 (+) 1707 WP_354688550.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MRK42_RS19240 (MRK42_19185) pilC 4192465..4193706 (+) 1242 WP_026456598.1 type II secretion system F family protein Machinery gene
  MRK42_RS19245 (MRK42_19190) pilD 4193773..4194648 (+) 876 WP_342013424.1 A24 family peptidase Machinery gene
  MRK42_RS19250 (MRK42_19195) coaE 4194652..4195266 (+) 615 WP_354688551.1 dephospho-CoA kinase -
  MRK42_RS19255 (MRK42_19200) zapD 4195304..4196026 (+) 723 WP_354688552.1 cell division protein ZapD -
  MRK42_RS19260 (MRK42_19205) yacG 4196036..4196230 (+) 195 WP_026457308.1 DNA gyrase inhibitor YacG -
  MRK42_RS19265 (MRK42_19210) mutT 4196293..4196688 (-) 396 WP_026457309.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62562.96 Da        Isoelectric Point: 5.5619

>NTDB_id=676369 MRK42_RS19235 WP_354688550.1 4190535..4192241(+) (pilB) [Aeromonas sp. 19NY04SH05-1]
MTSSPHSGLAISLAASSLISEEDSQRYLNQAKAQRKPFVAFLVENQILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLENETDVLGMEDIDESEI
SDLEVSDEGSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=676369 MRK42_RS19235 WP_354688550.1 4190535..4192241(+) (pilB) [Aeromonas sp. 19NY04SH05-1]
ATGACCTCTAGCCCCCATAGCGGCCTGGCGATAAGCCTGGCCGCCTCTTCGTTGATCAGCGAAGAGGATTCCCAGCGCTA
CCTGAATCAGGCCAAGGCCCAGCGCAAACCCTTCGTGGCATTTCTGGTGGAAAACCAGATCCTCGACAGCAAGGCGTTGG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAAAAGCTGATTGAGAAACATCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAAGATTTCGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAAGAAAACA
AGCTCACCACCGCCATCGGCAAGCTGCTGGAGAACGAGACCGACGTCCTCGGTATGGAGGATATCGACGAGTCGGAGATC
TCGGATCTTGAGGTCTCCGACGAGGGCAGCCGCCTCGACGAGAGCGTCAACACCACGGATGATGACGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGATCTGCACTTCGAGCCCTATGAGCAGAAGTACC
GGATCCGCTTTCGCATTGACGGCATCCTGCACGAGATTGCCACACCACCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGCCGCATCAAACTCAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTGATTCGTCTGCTGGACTCCTCGG
CGGCGCGGCTCAATATCGAACAGCTCGGCTTTGACGATCGGCAGAAAGCGCAATACCTGCGCGCCCTCTCCAAGCCCCAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTTTACACCGGCCTCAACATTCTCAACACCAC
AGAGGTGAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CGGGACTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGTCAGGATCCTGATGTGGTGATGGTCGGGGAAATACGGGAC
TTGGAAACCGCAGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGCAAGCTGTGCGATCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTTACC
CCGCAGCAACTGGCTGCGGGGCTAAGGTTGTTCAAACCGGTGGGTTGCAAGGAGTGCTCTGGCGGTTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAAAATATCGCAAAATTGATCATGCAGGGAGCCAACTCGCTGCAGATCGCCG
CCATCGCCCAGAAAGAGGGGATGCGGACCCTGCGCACCTCCGGGTTGGAAAAGGCCCGCCTGGGTGTCACCAGTCTGGCG
GAAGTGAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.81

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

53.996

99.12

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

50.348

100

0.509
  pilF Neisseria gonorrhoeae MS11

47.163

99.296

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

38.062

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375