Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KF715C_RS28710 Genome accession   NZ_AP015029
Coordinates   6226005..6227267 (-) Length   420 a.a.
NCBI ID   WP_016489623.1    Uniprot ID   -
Organism   Pseudomonas putida strain KF715     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6221005..6232267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF715C_RS35710 - 6222314..6222538 (-) 225 WP_132844974.1 hypothetical protein -
  KF715C_RS28695 (KF715C_ch55960) - 6222668..6224272 (-) 1605 WP_060516914.1 SPOR domain-containing protein -
  KF715C_RS28700 (KF715C_ch55970) aroB 6224288..6225385 (-) 1098 WP_024717572.1 3-dehydroquinate synthase -
  KF715C_RS28705 (KF715C_ch55980) aroK 6225482..6226000 (-) 519 WP_024717571.1 shikimate kinase AroK -
  KF715C_RS28710 (KF715C_ch55990) pilQ 6226005..6227267 (-) 1263 WP_016489623.1 type IV pilus secretin PilQ Machinery gene
  KF715C_RS28715 (KF715C_ch56000) - 6227191..6228207 (-) 1017 WP_095116155.1 pilus assembly protein PilP -
  KF715C_RS28720 (KF715C_ch56010) - 6228204..6228740 (-) 537 WP_096426873.1 PilN domain-containing protein -
  KF715C_RS28725 (KF715C_ch56020) pilM 6228737..6229618 (-) 882 WP_023048611.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45918.61 Da        Isoelectric Point: 4.9055

>NTDB_id=67576 KF715C_RS28710 WP_016489623.1 6226005..6227267(-) (pilQ) [Pseudomonas putida strain KF715]
MKNVMKWVLAALLPIPLVWATPYQGEPLSLNFQDVEVRSVLQVLADYAGINLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLAQRHEGNVLLVAPAAAFAPQPFDAHPVPDAQMQPLRRELIPIHHAKAAELVELLLTSLADDSTFTVRGSLGVDERT
NTLVAHQPADRLAELRQLVTQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGESLRPGKELFVDLGVERAGSSVGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVAFREASLSLEVTPQITPDNKV
IMAVRVTKDEPDYVNAVNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDVLQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=67576 KF715C_RS28710 WP_016489623.1 6226005..6227267(-) (pilQ) [Pseudomonas putida strain KF715]
ATGAAGAACGTGATGAAATGGGTGTTGGCGGCCCTGCTGCCGATCCCGCTGGTGTGGGCGACGCCGTATCAGGGTGAGCC
ACTGTCTTTGAATTTCCAGGATGTAGAGGTTCGCTCGGTGCTGCAGGTGCTGGCCGATTACGCGGGCATCAATCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGTTTGCAGGACGTGCCCTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGGCACAGCGGCACGAAGGCAATGTGTTGCTGGTGGCCCCTGCGGCAGCGTTTGCCCCGCAGCCGTTCGA
TGCCCATCCGGTGCCGGATGCGCAGATGCAGCCGTTACGCCGTGAACTGATACCGATCCATCATGCCAAGGCGGCGGAGC
TGGTTGAGTTGTTGCTGACCAGCCTGGCGGATGACAGCACCTTCACCGTTCGTGGCAGCCTGGGTGTCGATGAGCGCACC
AATACCCTGGTGGCGCATCAGCCGGCCGATCGCCTGGCCGAATTGCGGCAACTGGTTACGCAGCTGGACGTGCCGGTGCG
CCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGACTACGAGAAAAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TGTACGGGGAAAGCCTGCGCCCGGGCAAGGAACTGTTCGTCGACCTCGGCGTGGAGCGGGCGGGCAGCAGCGTCGGCCTG
GGCCTGCTGCGCGGTGACGTGTTGCTCGACCTGGAACTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCATCTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCAGCCAGA
GTGGCGCCACCTCGGTCGCCTTCCGCGAAGCCTCGCTTTCGCTGGAGGTGACCCCGCAGATTACACCTGACAACAAGGTA
ATCATGGCTGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCCGTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Bacterial type II/III secretion system short domain (Secretin_N)

(123-188)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.998

100

0.617
  pilQ Acinetobacter baumannii strain A118

42.241

100

0.467
  pilQ Acinetobacter baumannii D1279779

42.241

100

0.467
  comQ Acinetobacter baylyi ADP1

43.111

100

0.462
  pilQ Legionella pneumophila strain Lp02

42.143

100

0.421
  pilQ Legionella pneumophila strain ERS1305867

42.143

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.941

100

0.414
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.487

99.286

0.412
  pilQ Vibrio cholerae strain A1552

41.487

99.286

0.412

Multiple sequence alignment