Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTZ49_RS08460 Genome accession   NZ_CP094972
Coordinates   1767982..1769646 (-) Length   554 a.a.
NCBI ID   WP_264745117.1    Uniprot ID   -
Organism   Entomomonas sp. E2T0     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1762982..1774646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTZ49_RS08440 (MTZ49_08435) yacG 1764809..1765027 (-) 219 WP_264745113.1 DNA gyrase inhibitor YacG -
  MTZ49_RS08445 (MTZ49_08440) coaE 1765032..1765634 (-) 603 WP_264745114.1 dephospho-CoA kinase -
  MTZ49_RS08450 (MTZ49_08445) pilD 1765721..1766587 (-) 867 WP_264745115.1 prepilin peptidase Machinery gene
  MTZ49_RS08455 (MTZ49_08450) pilC 1766593..1767801 (-) 1209 WP_264745116.1 type II secretion system F family protein Machinery gene
  MTZ49_RS08460 (MTZ49_08455) pilB 1767982..1769646 (-) 1665 WP_264745117.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTZ49_RS08465 (MTZ49_08460) - 1769812..1770792 (-) 981 WP_264745118.1 two-component system response regulator -
  MTZ49_RS08470 (MTZ49_08465) - 1770797..1773403 (-) 2607 WP_264745119.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 61241.23 Da        Isoelectric Point: 5.3176

>NTDB_id=674631 MTZ49_RS08460 WP_264745117.1 1767982..1769646(-) (pilB) [Entomomonas sp. E2T0]
MSAQNPPLTGLPKLFVDNNILSENDMRQAMQQSKVEQLPLVTYLVQNKMASAKSIAILISEQLGLPLFDLSSLQIDDALK
SVVTEKLARQHRAIPLWKRGNSLFLGISDPSNQQGLRDIQFNTGLPVDAILVEEDKLTSLLDRFFDTEAEQVSTSPLQKP
GSGDDEKEDEAVVNFVNQMLLNAIRSGASDLHFEPYEKSYRVRFRSDGILQIKSRPPIELAPRIAARLKVMSDMDISERR
KPQDGRIKLKVSERKSIDFRVNTCPTLWGEKIVLRILDSSSAQMGIDSLGYEPEQKALYMEALSHPQGMILVTGPTGSGK
TVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFAAALRAFLRQDPDIIMVGEIRDLETAEIAIKAAQT
GHMVMSTLHTNSAPETLTRLMNMGVASFNIATSVNLIIAQRLARRLCPHCKQEMEIPHEALVKAGFPEDKIGNFTVYGPV
GCDRCNNGYKGRVGIYEVVKITPALQRIIMSEGNAIEIADEARKEGFADLRTSGLKKVMDGLTSLEEVNRVTSD

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=674631 MTZ49_RS08460 WP_264745117.1 1767982..1769646(-) (pilB) [Entomomonas sp. E2T0]
ATGAGCGCACAAAATCCTCCTTTAACAGGACTTCCAAAACTGTTTGTAGATAACAATATCTTATCAGAAAATGATATGCG
CCAAGCCATGCAACAATCAAAGGTAGAACAATTACCGCTTGTTACTTATTTAGTACAAAATAAAATGGCTAGTGCAAAAA
GCATTGCTATACTCATTTCAGAACAATTAGGATTACCTCTTTTTGATTTAAGCAGTTTACAAATAGATGATGCCTTAAAG
TCTGTAGTTACTGAAAAGCTAGCTCGTCAACACCGAGCAATCCCTCTATGGAAGCGTGGCAATAGTTTATTTTTAGGTAT
TTCTGACCCATCAAACCAACAGGGCTTAAGGGATATTCAATTTAATACAGGCTTACCTGTTGATGCAATTCTTGTTGAAG
AAGATAAATTAACTTCTTTACTGGACCGTTTTTTTGATACAGAAGCAGAACAAGTCAGTACTAGTCCCTTACAAAAACCT
GGTTCAGGTGATGATGAAAAAGAGGACGAAGCAGTTGTTAACTTTGTTAACCAAATGCTATTAAATGCTATACGTAGTGG
CGCATCTGACTTACATTTCGAACCTTATGAAAAGAGTTACCGAGTACGTTTCCGTAGTGACGGGATTTTACAGATAAAAT
CACGTCCGCCCATTGAACTCGCACCGCGTATTGCAGCTCGTTTAAAAGTAATGTCTGATATGGATATTTCTGAACGTCGG
AAACCTCAAGATGGTCGTATTAAATTAAAAGTTTCAGAAAGAAAATCCATCGATTTCCGGGTTAATACTTGCCCTACTTT
ATGGGGTGAAAAAATTGTATTACGGATATTAGACTCCTCTAGTGCACAAATGGGTATTGACTCTCTAGGTTATGAGCCTG
AACAAAAAGCACTCTATATGGAAGCTCTTAGTCATCCCCAAGGTATGATTTTAGTAACAGGACCAACGGGTTCTGGTAAA
ACGGTATCCCTCTATACAGGTTTAAATATACTTAATACACCAGATATTAATATCTCCACAGCAGAAGACCCTGTTGAAAT
TAACTTAGAAGGGATTAACCAAGTTAACGTTAACCCTAAACAAGGAATGGATTTTGCGGCAGCGTTACGGGCATTCTTAC
GTCAAGATCCAGATATTATTATGGTTGGTGAAATTCGTGACTTGGAAACAGCAGAAATTGCTATTAAAGCTGCTCAAACA
GGCCATATGGTAATGTCAACTTTACATACTAATAGTGCCCCTGAAACATTAACACGTCTAATGAATATGGGAGTTGCTTC
TTTTAATATTGCGACCTCTGTCAATCTAATTATTGCCCAACGATTAGCAAGACGTTTATGTCCACATTGTAAGCAAGAAA
TGGAAATCCCCCATGAAGCCTTAGTAAAAGCAGGATTTCCAGAAGATAAAATTGGTAATTTCACTGTTTACGGCCCTGTA
GGTTGTGACCGCTGTAATAATGGTTATAAAGGCCGTGTGGGTATCTATGAAGTAGTAAAAATAACTCCTGCTTTACAACG
TATTATTATGTCAGAAGGCAATGCTATTGAAATTGCTGATGAAGCAAGAAAAGAAGGTTTTGCGGACTTAAGAACCTCTG
GTCTCAAAAAAGTAATGGATGGTTTAACCAGTCTAGAAGAAGTTAATCGTGTTACATCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

100

0.614
  pilB Acinetobacter baylyi ADP1

59.68

100

0.606
  pilB Legionella pneumophila strain ERS1305867

51.224

100

0.529
  pilF Neisseria gonorrhoeae MS11

50.447

100

0.509
  pilB Vibrio cholerae strain A1552

48.571

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

48.393

100

0.489
  pilB Vibrio campbellii strain DS40M4

47.95

100

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.493

99.639

0.394
  pilF Thermus thermophilus HB27

38.121

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

37.297

100

0.374