Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SVA_RS03485 Genome accession   NZ_AP014936
Coordinates   704847..706565 (-) Length   572 a.a.
NCBI ID   WP_096458873.1    Uniprot ID   A0A1B4V1C5
Organism   Sulfurifustis variabilis strain skN76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699847..711565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVA_RS03460 (SVA_0705) argJ 699866..701083 (+) 1218 WP_096458858.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  SVA_RS03465 (SVA_0706) - 701093..702085 (+) 993 WP_096458861.1 Nudix family hydrolase -
  SVA_RS03470 (SVA_0707) coaE 702082..702684 (-) 603 WP_096458864.1 dephospho-CoA kinase -
  SVA_RS03475 (SVA_0708) pilD 702708..703565 (-) 858 WP_096458867.1 prepilin peptidase Machinery gene
  SVA_RS03480 (SVA_0709) pilC 703621..704847 (-) 1227 WP_096458870.1 type II secretion system F family protein Machinery gene
  SVA_RS03485 (SVA_0710) pilB 704847..706565 (-) 1719 WP_096458873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SVA_RS03490 (SVA_0711) - 706962..707384 (+) 423 WP_096458877.1 rubrerythrin family protein -
  SVA_RS03495 (SVA_0712) - 707454..708818 (+) 1365 WP_096458880.1 heterodisulfide reductase-related iron-sulfur binding cluster -
  SVA_RS03500 (SVA_0713) - 708815..709429 (+) 615 WP_197703352.1 DUF3501 family protein -
  SVA_RS03505 (SVA_0714) - 709518..710045 (+) 528 WP_096458883.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62392.72 Da        Isoelectric Point: 5.2457

>NTDB_id=67422 SVA_RS03485 WP_096458873.1 704847..706565(-) (pilB) [Sulfurifustis variabilis strain skN76]
MATVNPTTNLSGLALRLVRDNLLTPTDAERIQSEALASKMPFVSRLVESKKLDAGTVARIASEEFGVPLFDINALDLDSA
PVKLVDEKILRRHRVLPLFKRGTRLYVAVGDPTNLQALDEIKFHTGLGTDPILVEEDKLVTALEKCLEANDTTLMDMASD
DSLENLEIVAGQEEKDAAPAGEEGVNEAPIVKFVNKVLMDAINKGASDIHIEPYEKTYRVRYRMDGILHEVANPPLALSS
RIAARIKILSKLDIAERRVPQDGRIKMRISKNRAIDFRVSTLPCLWGEKLVLRILDPTSATLGVDKLGFEAEQKEAFLEA
VNRPYGMVLVTGPTGSGKTVTLYTALNILNTADSNISTAEDPVEMNVAGINQVNINEKAGLTFAGALRAFLRQDPDIIMV
GEIRDLETAEISIKAAQTGHLVLSTLHTNDAPATLTRLVNMGVPPFNIASSVHLIIAQRLGRRLCNNCKAPIQVPPEALI
KAGFRQNELDGLTVFGPVGCDACNNGYKGRVGIYQVMPVSEAMGDIIMRGGNQRDIELQAEKEGVSDLRRSGLRKVRDGI
TSLEEVEAVTNQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=67422 SVA_RS03485 WP_096458873.1 704847..706565(-) (pilB) [Sulfurifustis variabilis strain skN76]
ATGGCGACGGTCAATCCCACCACGAATCTCAGCGGACTGGCGCTCCGGCTGGTCCGCGATAATCTCCTCACGCCGACCGA
CGCCGAACGCATTCAGTCGGAGGCGCTCGCCAGCAAGATGCCCTTCGTCAGCCGCCTGGTCGAAAGCAAGAAGCTCGATG
CGGGCACGGTGGCCCGGATCGCGTCCGAGGAATTCGGCGTTCCGCTGTTCGACATCAACGCGCTGGACCTCGACAGCGCC
CCCGTCAAGCTGGTCGACGAGAAGATCCTGCGTCGGCATCGCGTCCTGCCCTTGTTCAAACGCGGCACACGCCTTTACGT
GGCCGTGGGCGATCCGACCAACCTGCAGGCCCTCGACGAGATCAAGTTCCATACCGGCCTTGGCACCGATCCGATCCTGG
TAGAGGAAGACAAGCTCGTCACCGCCCTCGAGAAGTGCCTGGAGGCGAACGACACGACGCTGATGGACATGGCGTCGGAC
GACAGTCTCGAAAACCTCGAGATCGTCGCGGGACAGGAGGAAAAGGATGCCGCTCCTGCCGGTGAAGAGGGCGTCAACGA
AGCGCCGATCGTCAAGTTCGTGAACAAGGTCCTGATGGACGCCATCAACAAGGGCGCATCGGACATACATATCGAACCGT
ATGAAAAGACCTACCGCGTGCGCTACCGGATGGACGGCATTCTGCACGAGGTGGCGAACCCCCCGCTTGCCCTCTCCAGC
CGCATCGCGGCACGCATCAAGATCCTCTCCAAGCTCGACATCGCCGAGCGCCGGGTACCGCAGGACGGCCGCATCAAGAT
GCGCATCTCCAAGAACCGCGCGATCGACTTTCGTGTGAGCACGCTGCCCTGCCTCTGGGGCGAAAAGCTGGTGCTCCGTA
TCCTCGACCCGACATCGGCGACGCTTGGCGTCGACAAACTGGGTTTCGAGGCCGAGCAGAAAGAGGCGTTCCTGGAGGCG
GTCAACCGGCCGTACGGCATGGTGCTCGTGACCGGACCGACCGGCAGCGGCAAGACGGTAACCCTCTACACCGCCCTCAA
CATCCTCAATACCGCCGACTCCAACATCTCCACCGCGGAAGACCCGGTGGAAATGAACGTGGCGGGCATCAACCAGGTGA
ACATCAACGAGAAGGCAGGCCTGACTTTTGCCGGGGCGCTTCGCGCCTTTCTCCGCCAGGACCCCGACATCATCATGGTC
GGTGAAATACGCGACCTCGAGACTGCCGAGATCTCGATCAAAGCGGCGCAGACCGGCCACCTGGTGCTGTCGACGCTTCA
TACGAACGATGCCCCCGCGACCCTGACCCGCCTGGTCAACATGGGGGTGCCTCCGTTCAACATCGCGTCCTCGGTGCACC
TCATCATCGCGCAGCGACTCGGCCGCCGGCTGTGCAACAACTGCAAGGCGCCGATCCAGGTTCCCCCCGAGGCCCTCATC
AAGGCCGGCTTTCGGCAAAACGAGCTCGACGGTCTGACCGTCTTCGGACCCGTCGGTTGCGACGCCTGCAACAACGGCTA
TAAAGGGCGGGTGGGCATCTATCAGGTGATGCCGGTTTCGGAGGCGATGGGCGACATCATCATGCGCGGCGGCAACCAGC
GGGACATCGAGCTGCAGGCGGAAAAAGAAGGCGTCTCCGACCTGCGGCGCTCCGGACTGCGCAAGGTGCGCGACGGCATC
ACGAGCCTCGAGGAAGTCGAGGCGGTGACCAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4V1C5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.766

100

0.579
  pilB Acinetobacter baumannii D1279779

54.355

100

0.545
  pilB Acinetobacter baylyi ADP1

53.136

100

0.533
  pilF Neisseria gonorrhoeae MS11

49.303

100

0.495
  pilB Vibrio cholerae strain A1552

49.558

98.776

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

47.865

98.252

0.47
  pilB Vibrio campbellii strain DS40M4

46.181

100

0.465
  pilF Thermus thermophilus HB27

39.107

97.902

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

39.162

95.979

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.265

92.483

0.372

Multiple sequence alignment