Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SVA_RS03475 Genome accession   NZ_AP014936
Coordinates   702708..703565 (-) Length   285 a.a.
NCBI ID   WP_096458867.1    Uniprot ID   A0A1B4VD33
Organism   Sulfurifustis variabilis strain skN76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697708..708565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVA_RS03460 (SVA_0705) argJ 699866..701083 (+) 1218 WP_096458858.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  SVA_RS03465 (SVA_0706) - 701093..702085 (+) 993 WP_096458861.1 Nudix family hydrolase -
  SVA_RS03470 (SVA_0707) coaE 702082..702684 (-) 603 WP_096458864.1 dephospho-CoA kinase -
  SVA_RS03475 (SVA_0708) pilD 702708..703565 (-) 858 WP_096458867.1 prepilin peptidase Machinery gene
  SVA_RS03480 (SVA_0709) pilC 703621..704847 (-) 1227 WP_096458870.1 type II secretion system F family protein Machinery gene
  SVA_RS03485 (SVA_0710) pilB 704847..706565 (-) 1719 WP_096458873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SVA_RS03490 (SVA_0711) - 706962..707384 (+) 423 WP_096458877.1 rubrerythrin family protein -

Sequence

Protein


Download         Length: 285 a.a.        Molecular weight: 31333.11 Da        Isoelectric Point: 8.4751

>NTDB_id=67420 SVA_RS03475 WP_096458867.1 702708..703565(-) (pilD) [Sulfurifustis variabilis strain skN76]
MLAAYPAALSLLAGVLGLLVGSFLNVVIHRLPIMLERRWRAQCQELAGQPVEPTERYDLVVPRSRCPRCGHRISAIENIP
LLSFLWLRGKCAECAQPISWRYPSVELVSGLLSAAVAWHFGFSLAALGALLLTWSLIALSFIDYDHQLLPDDITLPLLWL
GLGLNLAGTYVSLSSAVIGAFAGYLSLWLVYQGFKLLTGREGMGYGDFKLFAAFGAWLGWQQLPLVILLSSFLGALIGLG
FILLRGRDRSLPIPFGPFLCLAGWVALLWGDTITRAYLQFARFPA

Nucleotide


Download         Length: 858 bp        

>NTDB_id=67420 SVA_RS03475 WP_096458867.1 702708..703565(-) (pilD) [Sulfurifustis variabilis strain skN76]
ATGCTCGCCGCCTACCCGGCTGCACTGTCCCTGCTCGCCGGTGTTCTGGGTCTTCTCGTCGGGAGCTTCCTGAACGTCGT
CATCCACCGGCTTCCGATCATGCTGGAGCGCCGCTGGCGGGCCCAATGCCAGGAGCTCGCCGGACAGCCGGTCGAGCCGA
CGGAACGCTACGACCTCGTCGTGCCGCGTTCACGCTGTCCGCGCTGCGGGCATCGCATCTCCGCGATCGAAAACATCCCG
CTCCTGTCGTTTTTGTGGTTGCGGGGCAAGTGCGCGGAATGCGCGCAACCGATCTCCTGGCGCTATCCCTCCGTGGAGCT
CGTCTCGGGCCTCCTGTCCGCTGCCGTCGCTTGGCACTTCGGCTTCTCGCTCGCCGCCCTCGGCGCCCTGCTGCTGACCT
GGTCCCTGATCGCTCTCAGCTTCATCGACTACGACCATCAGCTCCTGCCCGATGACATCACGCTTCCGCTCCTGTGGCTC
GGGCTCGGGCTGAATCTCGCGGGCACCTATGTGTCGCTTTCTTCGGCGGTCATCGGCGCATTCGCCGGATACCTCAGCCT
CTGGCTGGTTTACCAGGGATTCAAGCTGCTCACGGGCCGCGAGGGCATGGGTTACGGCGACTTCAAGCTGTTCGCCGCGT
TCGGCGCATGGCTGGGGTGGCAGCAGCTGCCGCTGGTCATACTGCTGTCGTCCTTCCTCGGTGCCTTGATCGGCCTCGGT
TTCATCCTGCTGCGCGGCCGCGATCGCAGCCTTCCCATTCCGTTCGGCCCCTTCCTGTGTCTCGCCGGCTGGGTCGCGCT
CCTCTGGGGCGACACCATCACCCGGGCGTATCTGCAGTTTGCACGGTTTCCGGCCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(15-120)

Type IV leader peptidase family (Peptidase_A24)

(131-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4VD33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.317

97.544

0.53
  pilD Vibrio campbellii strain DS40M4

52.347

97.193

0.509
  pilD Acinetobacter baumannii D1279779

52.555

96.14

0.505
  pilD Acinetobacter nosocomialis M2

52.555

96.14

0.505
  pilD Neisseria gonorrhoeae MS11

51.311

93.684

0.481

Multiple sequence alignment