Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTP21_RS11830 Genome accession   NZ_CP094880
Coordinates   2620878..2622563 (+) Length   561 a.a.
NCBI ID   WP_243974788.1    Uniprot ID   -
Organism   Vibrio natriegens strain WPAGA4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2615878..2627563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP21_RS11810 pdhR 2617453..2618220 (-) 768 WP_014232947.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MTP21_RS11815 ampD 2618630..2619175 (-) 546 WP_243974775.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MTP21_RS11820 nadC 2619268..2620155 (+) 888 WP_243974777.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MTP21_RS11825 - 2620414..2620878 (+) 465 WP_243974779.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MTP21_RS11830 pilB 2620878..2622563 (+) 1686 WP_243974788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTP21_RS11835 pilC 2622594..2623817 (+) 1224 WP_243974790.1 type II secretion system F family protein Machinery gene
  MTP21_RS11840 pilD 2623885..2624754 (+) 870 WP_243974794.1 A24 family peptidase Machinery gene
  MTP21_RS11845 coaE 2624755..2625369 (+) 615 WP_014232954.1 dephospho-CoA kinase -
  MTP21_RS11850 zapD 2625396..2626136 (+) 741 WP_014232955.1 cell division protein ZapD -
  MTP21_RS11855 yacG 2626210..2626404 (+) 195 WP_014232956.1 DNA gyrase inhibitor YacG -
  MTP21_RS11860 rplS 2626771..2627124 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62098.87 Da        Isoelectric Point: 5.7436

>NTDB_id=673890 MTP21_RS11830 WP_243974788.1 2620878..2622563(+) (pilB) [Vibrio natriegens strain WPAGA4]
MHTNLSTILRQMGLINLHQEESLIEQVNASGISMPEALVNSGIFTTSELATHLSSIFGLPCESLSHYDYTALCQKLGLRE
LITNHHALPLERTPSTLTLAIADPTNQQAEDDFRFATGLQVDLVIADFSELDAAIRRLYGRALGQERAGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRMPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLTNMGVESYNLASSLSLIIAQRLARKLCPHCKQPQEVTAALQHIGINATDQ
IYQANPNGCNHCTHGYSGRTGIYEVMRFDETLSEALIKGASVHQLEKLAIRNGMSTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=673890 MTP21_RS11830 WP_243974788.1 2620878..2622563(+) (pilB) [Vibrio natriegens strain WPAGA4]
ATGCACACAAACCTCTCAACGATTCTTCGTCAAATGGGATTAATCAATTTACACCAAGAAGAATCGTTAATAGAGCAAGT
CAACGCTTCCGGTATTTCGATGCCGGAAGCGTTAGTTAACTCTGGTATATTCACTACTAGCGAACTCGCGACTCACCTTA
GTTCAATCTTTGGTTTACCCTGCGAATCCTTATCTCACTATGATTACACCGCCCTGTGCCAAAAATTGGGGCTGCGTGAG
CTGATCACGAATCACCATGCTCTACCACTGGAGCGAACGCCTTCGACGTTAACTCTCGCCATTGCCGACCCAACGAACCA
ACAAGCTGAAGATGATTTTCGCTTTGCTACCGGATTACAAGTCGACTTAGTTATCGCTGATTTTTCGGAGCTTGATGCTG
CGATTCGCCGTTTGTACGGTCGTGCGTTAGGTCAGGAGAGAGCCGGCTTAAAAGAGATCAATCAAGAAGAATTGGCTAAT
TTGGTTGACGTCGGTGCTGATGAAATCGATAACATCGAAGACTTAAGTCAGGACGAGTCACCGGTTAGTCGCTATATCAA
CCAAATTTTACTTGATGCGGTACGTAAAGGCGCATCAGATATCCATTTTGAGCCCTATGAAAAGATGTACCGAGTCCGGC
TGCGCTGCGACGGTATCTTGATTGAAACCCAACAACCACCGAGCCATTTAAGTCGACGCTTATCGGCTCGAATCAAAATT
CTGTCCAAGCTTGATATTGCTGAAAGGCGTATGCCGCAAGACGGTCGAATAAAGTTAAAGCTTAATCAAGACACTGCCAT
TGATATGCGAGTGTCCACCTTACCGACTCTATTCGGAGAAAAAATCGTATTACGACTGCTCGACAGCAGTTCAGCTTCTC
TGGATATAGATAAACTAGGCTACAGTGACCAGCAAAAGCAGCTGTACTTAGATGCTCTGCGTCGTCCACAAGGCATGATT
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTCTCGTTGTACACAGGGCTGAGCATACTCAATAAACCAGAGATCAA
TATTTCTACGGCTGAAGACCCGGTCGAAATTAACCTCTCCGGAATTAATCAGGTTCAGGTTCAACCCAAGATAGGCTTTG
GTTTCGCCGAGGCACTGCGCTCTTTCCTGCGTCAAGACCCTGATGTGGTCATGGTGGGAGAAATCCGCGATTTGGACACA
GCAGAAATCGCGATTAAGGCCTCACAAACCGGACACTTAGTCTTGTCGACACTCCACACAAATTCAGCCGCTGAAACCGT
TATCCGCCTAACGAATATGGGCGTTGAAAGCTACAACCTCGCCTCGTCGCTCAGCCTTATTATTGCCCAGCGCTTAGCCC
GTAAACTTTGCCCGCACTGTAAACAACCTCAAGAGGTTACCGCCGCATTACAACATATTGGCATTAACGCAACTGACCAG
ATTTATCAAGCCAACCCAAATGGCTGCAACCACTGTACACACGGCTATTCGGGACGAACTGGCATCTATGAGGTGATGCG
TTTCGATGAAACCCTATCCGAAGCACTGATTAAAGGGGCCTCGGTACATCAACTGGAAAAACTCGCCATCCGTAACGGGA
TGAGCACACTACAAATGTCAGGTATTGAAAAGCTTAAACAAGGCGTCACAAGCTTTAGCGAACTGCAGCGCGTGCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

89.127

100

0.891
  pilB Vibrio campbellii strain DS40M4

86.453

100

0.865
  pilB Vibrio cholerae strain A1552

72.954

100

0.731
  pilB Legionella pneumophila strain ERS1305867

47.611

100

0.48
  pilB Acinetobacter baumannii D1279779

50.857

93.583

0.476
  pilB Acinetobacter baylyi ADP1

47.576

99.287

0.472
  pilF Neisseria gonorrhoeae MS11

44.149

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

37.522

99.287

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.011

100

0.371
  pilF Thermus thermophilus HB27

36.477

100

0.365