Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MT343_RS04465 Genome accession   NZ_CP094879
Coordinates   990475..992172 (-) Length   565 a.a.
NCBI ID   WP_145469679.1    Uniprot ID   -
Organism   Thalassolituus sp. C2-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 985475..997172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MT343_RS04440 - 985852..987375 (+) 1524 WP_145469684.1 hypothetical protein -
  MT343_RS04445 yacG 987451..987651 (-) 201 WP_145469683.1 DNA gyrase inhibitor YacG -
  MT343_RS04450 coaE 987706..988305 (-) 600 WP_145469682.1 dephospho-CoA kinase -
  MT343_RS04455 pilD 988322..989200 (-) 879 WP_145469681.1 A24 family peptidase Machinery gene
  MT343_RS04460 pilC 989200..990426 (-) 1227 WP_145469680.1 type II secretion system F family protein Machinery gene
  MT343_RS04465 pilB 990475..992172 (-) 1698 WP_145469679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MT343_RS19580 - 992307..992951 (-) 645 WP_145469678.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MT343_RS04475 - 993205..993870 (-) 666 WP_145469677.1 hypothetical protein -
  MT343_RS04480 nadC 993905..994768 (-) 864 WP_145469676.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MT343_RS04485 - 994743..996125 (-) 1383 WP_145469675.1 sodium:alanine symporter family protein -
  MT343_RS04490 ampD 996256..996813 (+) 558 WP_145469674.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61628.55 Da        Isoelectric Point: 4.8991

>NTDB_id=673849 MT343_RS04465 WP_145469679.1 990475..992172(-) (pilB) [Thalassolituus sp. C2-1]
MAVLTGLPRRIVEQNLVNSQQAQEIINKAKQDKISFVAAAVASGYIKSRAIALLASEEFGLPLLDLSAFDFDLMPKELVS
RELIEKHRVLPLFQRGNRLFVAQSDPSAMLAIDEIKFNTGMTVEAVLAEEDKLGSAVDKFLAANDNSFGDLDGELDDVDL
EDPDARDKAQETEKDSSGDDAPIVRFVNKMLLDAIKGGASDIHFEPYEKRYRVRYRTDGMLHEVASPPVNLAGKIAARLK
VMSQMDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDMLGYEADQKTLYLDALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDLD
TASIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCPSCKVPAEIPKELLLEAGFTDE
QLQTAEIYKPVGCEQCKGGYKGRVGIYEVVRITDAISRIIMEGGNSIEISDAARKEGFNDLRTSALQKVAQGVTSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=673849 MT343_RS04465 WP_145469679.1 990475..992172(-) (pilB) [Thalassolituus sp. C2-1]
ATGGCCGTCTTAACCGGCTTACCACGCCGTATCGTTGAGCAGAATCTGGTGAACAGTCAGCAGGCTCAGGAAATCATCAA
TAAGGCAAAGCAGGACAAAATCAGCTTTGTTGCCGCCGCCGTTGCATCGGGTTACATAAAATCACGCGCCATTGCACTGC
TGGCTTCTGAAGAGTTTGGTCTCCCCCTGTTGGACCTCAGTGCCTTTGATTTTGATCTGATGCCCAAAGAACTGGTCAGC
CGGGAGCTGATCGAGAAACACCGGGTGCTGCCACTGTTTCAGCGCGGAAACCGTCTTTTTGTTGCGCAAAGCGACCCATC
CGCCATGCTGGCAATCGACGAAATCAAGTTTAATACCGGCATGACCGTTGAAGCGGTACTGGCTGAAGAAGATAAACTCG
GCAGTGCCGTCGATAAGTTTCTGGCCGCTAACGACAACAGCTTTGGTGACCTGGACGGCGAGCTCGACGATGTGGATCTG
GAAGACCCGGACGCCCGGGATAAAGCCCAGGAAACCGAGAAAGATTCCTCCGGCGACGACGCTCCGATCGTACGTTTTGT
CAATAAAATGCTGCTCGATGCTATTAAAGGCGGCGCTTCCGATATTCATTTTGAGCCTTATGAAAAGCGCTACCGGGTAC
GTTACCGTACCGATGGTATGTTGCATGAAGTCGCCAGCCCGCCAGTCAACCTTGCCGGCAAGATTGCTGCGCGCTTAAAG
GTGATGTCACAGATGGATATCTCTGAGCGGCGTGTTCCCCAGGATGGCCGTATCAAGATGAAGCTGTCCAAAACCAAGGC
CATCGACTTCCGCGTCAATACCCTGCCAACCCTGTGGGGCGAAAAGATCGTACTGCGTATTCTCGATCCTTCTTCAGCCA
AACTGGGGATTGATATGCTCGGCTATGAAGCCGATCAGAAAACCCTGTATCTGGATGCCCTGGCCAAGCCTCAGGGGATG
ATTCTGGTTACCGGCCCGACCGGCTCCGGTAAAACGGTATCCCTGTATACCGGTCTGAATATCCTTAACGTGGAAGGCAC
CAATATATCCACTGCCGAAGACCCGGTGGAAATTAACCTCGAAGGTATTAACCAGGTAAACGTGAACGCCCGCGTAGGCC
TCGACTTTGCAGCGGCTCTGCGTTCCTTCCTGCGTCAGGACCCGGACGTGGTGATGGTAGGGGAAATCCGGGATCTGGAT
ACTGCCTCCATCGCGATTAAAGCAGCCCAGACTGGTCACTTAGTGCTTTCCACACTACACACTAACAGTGCTGCCGAAAC
CCTGACCCGTCTGGCCAACATGGGCGTGCCCTCGTTCAATATTGCCACCTCCGTCAGTCTGATTATCGCTCAACGATTAG
CACGCCGCCTTTGCCCAAGTTGCAAGGTTCCGGCAGAAATACCTAAAGAGCTGCTTTTGGAAGCTGGCTTTACGGATGAG
CAGCTGCAAACCGCTGAGATTTATAAGCCGGTTGGTTGCGAACAATGTAAAGGCGGATACAAAGGCCGTGTCGGTATTTA
TGAAGTAGTTAGAATAACCGATGCCATCTCCCGTATTATCATGGAGGGCGGTAACTCGATTGAGATTTCTGATGCGGCGC
GCAAAGAAGGCTTTAACGATTTGCGTACATCGGCGCTGCAAAAGGTTGCCCAAGGGGTTACCAGCCTGGAAGAAGCTAAC
CGGGTAACCACCGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.832

100

0.628
  pilB Acinetobacter baylyi ADP1

61.348

99.823

0.612
  pilB Legionella pneumophila strain ERS1305867

54.816

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.971

98.761

0.513
  pilB Vibrio campbellii strain DS40M4

50.177

100

0.503
  pilB Vibrio cholerae strain A1552

50.446

99.292

0.501
  pilF Neisseria gonorrhoeae MS11

49.468

99.823

0.494
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.513

94.513

0.373
  pilF Thermus thermophilus HB27

36.602

100

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

38.534

94.159

0.363