Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SCL_RS02770 Genome accession   NZ_AP014879
Coordinates   555919..557640 (-) Length   573 a.a.
NCBI ID   WP_172425894.1    Uniprot ID   -
Organism   Sulfuricaulis limicola strain HA5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550919..562640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCL_RS02750 (SCL_0561) argJ 551783..553003 (+) 1221 WP_096359781.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  SCL_RS02755 (SCL_0562) coaE 553134..553742 (-) 609 WP_096359783.1 dephospho-CoA kinase -
  SCL_RS02760 (SCL_0563) pilD 553753..554637 (-) 885 WP_096359785.1 prepilin peptidase Machinery gene
  SCL_RS02765 (SCL_0564) pilC 554689..555915 (-) 1227 WP_096359787.1 type II secretion system F family protein Machinery gene
  SCL_RS02770 (SCL_0565) pilB 555919..557640 (-) 1722 WP_172425894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SCL_RS02775 (SCL_0566) - 558098..558520 (+) 423 WP_096359789.1 rubrerythrin family protein -
  SCL_RS02780 (SCL_0567) - 558658..560004 (+) 1347 WP_096359791.1 heterodisulfide reductase-related iron-sulfur binding cluster -
  SCL_RS02785 (SCL_0568) - 560039..560644 (+) 606 WP_420823634.1 DUF3501 family protein -
  SCL_RS02790 (SCL_0569) - 560738..561307 (+) 570 WP_148664957.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62211.61 Da        Isoelectric Point: 5.5935

>NTDB_id=67213 SCL_RS02770 WP_172425894.1 555919..557640(-) (pilB) [Sulfuricaulis limicola strain HA5]
MATASTASNLSGLALRLVRDNLLSPADAERIQTEALSKKTSFVTQLVESKKLDSATIAKVASEDFGIPLYEINSLDLEMA
PVKLVDEKIIRRHHVLPLFKRGTRLFVAVADPTNLQALDEIKFHTGLGTEPILVEEAKLAVIIEKSLDAQDTSLADLSGD
DSLENLEIVAGEEDEAKGAAGGEEGVNDAPVVKFVNKVLMDAINRGASDIHIEPYEKIYRVRYRQDGILHEVANPPIALS
ARIAARIKILSRLDIAERRVPQDGRIKMRISKNRAIDFRVSTLPTLWGEKIVMRILDPTSATLGVEKLGFEDFQKQLFLE
AVHRPYGMVLVTGPTGSGKTVTLYTAVNILNTPDTNISTAEDPVEINLSGVNQVNINDKAGLNFASALRAFLRQDPDVIL
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPSTLTRLANMGVPPFNIASAVNLIVAQRLARRLCAHCKKPLDIPKPAL
LKAGFREEDLPGLVVYGPVGCDACNGGYKGRVGIYQVMPVTEEIGAIIMRGGNQLDIEKQARKEGVPDLRQAGLMKIKAG
VTSLEEVEAATNE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=67213 SCL_RS02770 WP_172425894.1 555919..557640(-) (pilB) [Sulfuricaulis limicola strain HA5]
ATGGCAACCGCAAGCACGGCAAGCAACCTGAGCGGCCTCGCACTGAGGCTGGTGCGCGACAATCTGCTCAGTCCGGCGGA
CGCGGAACGCATCCAGACAGAAGCGCTCTCCAAAAAAACTTCCTTTGTCACGCAACTGGTCGAAAGCAAGAAACTCGACA
GCGCCACCATCGCCAAGGTGGCATCCGAGGATTTCGGCATACCGCTGTATGAAATCAACTCGCTCGATCTGGAAATGGCC
CCGGTCAAGCTGGTCGATGAAAAAATCATCCGGCGCCATCATGTCCTGCCCCTGTTCAAACGCGGGACGAGACTTTTTGT
CGCCGTCGCCGACCCCACCAATCTGCAGGCTCTGGATGAAATCAAGTTTCACACGGGGCTTGGCACGGAGCCGATCCTCG
TCGAAGAGGCCAAGCTCGCTGTCATCATCGAAAAGTCACTGGACGCGCAAGATACGAGCTTGGCCGATCTGTCCGGCGAT
GACAGCCTGGAAAACCTCGAAATCGTCGCTGGCGAGGAAGATGAGGCAAAGGGTGCCGCTGGCGGCGAGGAGGGCGTCAA
CGATGCTCCGGTCGTCAAATTCGTCAACAAGGTTCTGATGGACGCCATCAACCGCGGCGCCTCGGATATCCATATCGAGC
CGTACGAAAAAATCTACCGCGTGCGCTACCGGCAGGACGGCATATTGCACGAAGTGGCCAATCCGCCGATAGCCTTGTCC
GCCCGCATCGCCGCGCGCATCAAGATTCTTTCCAGGCTCGATATTGCGGAACGACGTGTTCCGCAGGACGGCCGCATCAA
GATGCGCATATCGAAGAATCGCGCGATCGATTTCCGTGTCAGTACGCTGCCAACGCTTTGGGGCGAGAAAATCGTGATGC
GTATTCTCGACCCGACCTCCGCCACGCTCGGCGTGGAAAAACTTGGGTTCGAGGATTTCCAGAAACAACTGTTCCTTGAG
GCCGTCCATCGCCCTTACGGCATGGTGCTGGTCACCGGGCCGACCGGTAGCGGCAAAACCGTCACCCTCTACACGGCGGT
TAACATACTCAACACACCGGATACCAATATTTCCACGGCGGAAGACCCGGTGGAAATCAACCTGTCCGGCGTCAACCAGG
TAAACATCAACGACAAGGCCGGACTCAATTTTGCCTCGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTCATCCTC
GTCGGCGAAATCCGCGATCTTGAAACGGCCGAGATTGCCATCAAGGCGGCACAAACGGGCCACATGGTGCTTTCCACCCT
GCACACGAACGATGCGCCATCCACGCTGACCCGTCTCGCCAACATGGGGGTACCGCCGTTCAACATCGCTTCGGCGGTCA
ATCTGATTGTTGCCCAACGCCTGGCGCGGCGACTGTGCGCGCATTGCAAGAAACCCCTCGACATCCCCAAACCGGCATTG
CTAAAGGCCGGTTTCAGGGAAGAAGACCTTCCCGGTCTCGTTGTTTATGGCCCGGTTGGTTGCGATGCCTGCAATGGCGG
CTATAAAGGACGTGTGGGTATCTATCAGGTCATGCCGGTGACGGAAGAAATCGGCGCCATCATCATGCGCGGTGGCAACC
AGCTGGATATCGAAAAGCAGGCCAGAAAGGAAGGCGTTCCCGATCTGCGGCAGGCAGGTCTGATGAAGATCAAGGCTGGT
GTCACCAGCCTGGAAGAAGTCGAAGCGGCAACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.944

100

0.572
  pilB Acinetobacter baumannii D1279779

54.389

100

0.551
  pilB Acinetobacter baylyi ADP1

52

100

0.522
  pilF Neisseria gonorrhoeae MS11

50.435

100

0.506
  pilB Vibrio cholerae strain A1552

49.912

98.604

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

48.319

98.604

0.476
  pilB Vibrio campbellii strain DS40M4

46.597

100

0.466
  pilF Thermus thermophilus HB27

40.493

99.127

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.199

93.194

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

39.099

96.859

0.379

Multiple sequence alignment