Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MMY79_RS17670 Genome accession   NZ_CP094657
Coordinates   3812437..3814149 (+) Length   570 a.a.
NCBI ID   WP_252610626.1    Uniprot ID   -
Organism   Acinetobacter sp. XS-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3807437..3819149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMY79_RS17635 (MMY79_17630) nusA 3808324..3809808 (-) 1485 WP_252610618.1 transcription termination factor NusA -
  MMY79_RS17640 (MMY79_17635) rimP 3809845..3810369 (-) 525 WP_252610620.1 ribosome maturation factor RimP -
  MMY79_RS17660 (MMY79_17655) secG 3811009..3811338 (-) 330 WP_252610622.1 preprotein translocase subunit SecG -
  MMY79_RS17665 (MMY79_17660) tpiA 3811351..3812145 (-) 795 WP_252610624.1 triose-phosphate isomerase -
  MMY79_RS17670 (MMY79_17665) pilB 3812437..3814149 (+) 1713 WP_252610626.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMY79_RS17675 (MMY79_17670) pilC 3814177..3815403 (+) 1227 WP_252610628.1 type II secretion system F family protein Machinery gene
  MMY79_RS19430 pilD 3815403..3816263 (+) 861 WP_289781518.1 A24 family peptidase Machinery gene
  MMY79_RS17690 (MMY79_17685) coaE 3816265..3816861 (+) 597 WP_252610630.1 dephospho-CoA kinase -
  MMY79_RS17695 (MMY79_17690) - 3816858..3817772 (-) 915 WP_252610632.1 DMT family transporter -
  MMY79_RS17700 (MMY79_17695) rlmB 3817808..3818557 (-) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MMY79_RS17705 (MMY79_17700) - 3818660..3818986 (-) 327 WP_252610634.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63407.75 Da        Isoelectric Point: 4.9673

>NTDB_id=670441 MMY79_RS17670 WP_252610626.1 3812437..3814149(+) (pilB) [Acinetobacter sp. XS-4]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQAALDAAKRAKQDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLVQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPTDVPKQSLLEMGF
TEQDLANPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=670441 MMY79_RS17670 WP_252610626.1 3812437..3814149(+) (pilB) [Acinetobacter sp. XS-4]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAGCAGCATTAGATGCAGCAAAAAGAGCTAAACAGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAACCCCAGCAATATAGAAGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAACCCGTTATTG
TCGAGCACCATAAACTTGAGAAAATTCTGACGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGCGATGAAGAATTT
GATCTTGATGTAGATCTTGATACCTCAACCGCTCCAGAAGAGGATGAGGATACTCCACAAGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGAGCTTCTGATTTACATTTCGAACCCTATGAAAAATCCT
ATCGGGTCCGCTATCGGGTGGATGGCGTTTTGCGTCAAATTGCAAATCCACCTCTGCAACTGGCGAATCGTTTGGCTTCA
CGCTTGAAAGTAATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAACTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAGAAGCTAGTTCTACGTATTCTCGATCCAT
CGAGTGCCATGCTGGGAATTGATGCTTTAGGATATGAAGAAGAGCAAAAAGCGCTTTTCATGGAAGCATTAGATAAGCCT
CAAGGTATGCTTTTAATTACTGGCCCAACAGGTTCTGGTAAAACAGTATCTTTATATACGGGCCTCAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTGACTTTTTCAGCTGCACTTAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCTGCTCAGACAGGTCATATGGTGATGTCTACACTACATACCAACAGTGC
CCCTGAAACACTGACTCGTCTACGCAATATGGGGGTTCCGTCTTTCAATATTGCGACATCGGTCAATCTGGTTATTGCTC
AGCGTTTGGCACGTCGGTTATGTCCACAATGTAAAGTGCCTACTGATGTCCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAGCTAATCCCGATTTTCAAGTTTTTCAACCAGTCGGTTGTCCAGAATGTCGTGAGGGTTACAAAGG
TCGAGTTGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.211

100

0.942
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

52.465

99.649

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

93.509

0.486
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio campbellii strain DS40M4

51.252

91.053

0.467
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.852

98.07

0.361